Gustatory dysfunction from apoptotic abnormality in zinc-deficient subjects' : pathological physiology and clinical appearances

缺锌受试者凋亡异常导致的味觉功能障碍：病理生理学和临床表现

• 批准号: 15390629
• 负责人: NAKASHIMA Kiyohito
• 金额: $ 6.66万
• 依托单位: Asahi University School of Dentistry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390629/
• 关键词: zinc deficiency; rats; mice; gustatory dysfunction; salt preference; behavioral response; conditioned taste aversion; hormonal imbalance; ホルモン調節; 味覚神経応答; ナトリウム代謝; アルドステロン; 飲水行動; 味覚障害; アポトーシス

In this study, we investigated the effects of Zn deficiency on taste sensitivity and various salts intakes by behavioral, electrophysiological and biochemical studies in rodents. 1.Behavioral studies using the 48-h two-bottle tests showed enhanced preference for NaCl in Zn deficient rats (ZnD-rats). The enhanced preference for NaCl in ZnD-rats was not observed when 10 min two-bottle test was used. The 48-h two-bottle test in ZnD-rats also showed that transection of the taste nerves did not affect the NaCl preference. The conditioned taste aversion tests showed similar generalization patterns between control and ZnD-rats for each of the four basic taste substances. In mice, the 48-h two-bottle test and lick rate test showed that aversiveness for CaCl_2 was larger in ZnD-mice than in the control. Changes in the behavioral responses to CaCl_2 were recovered by feeding the ZnD-mice with control diet for 1 week. The results suggest that taste may not play a role for enhanced NaCl preference in ZnD-rats, and that ZnD-rats have the ability to discriminate the basic taste qualities. The results also suggest a possibility that zinc deficiency may alter the taste-guided drinking behavior of CaCl_2 in mice. 2.Electrophysiological recordings of the chorda tympani nerve responses in rats showed no significant differences in concentration-response relationships for various taste stimuli between the two groups, suggesting that Zn-deficiency has negligible effects on taste sensitivities for the taste stimuli. 3.Serum Na levels in the ZnD-rats tended to be lower and more variable than those of control and pair-fed rats. A daily excretion of urinary Na tended to increase in the ZnD-rats. In the serum of ZnD-rats, angiotensin II and Ca levels significantly decreased, whereas aldosterone levels significantly increased. The results suggest that the disorder of sodium metabolism with upregulation of aldosterone is involved in Zn deficiency.

在这项研究中，我们研究了Zn缺乏对啮齿动物中行为，电生理和生化研究的味觉敏感性和各种盐分的影响。 1.使用48小时两瓶测试的行为研究表明，在锌不足大鼠（ZnD大鼠）中对NaCl的偏好增强。当使用10分钟两瓶测试时，未观察到ZnD鼠标中NaCl的偏好增强。 ZnD大鼠中的48小时两瓶测试还表明，味觉神经的横向不会影响NaCl的偏好。对于四种基本味道中的每一种，条件味觉厌恶测试显示了对照和ZnD鼠之间的相似概括模式。在小鼠中，48小时的两瓶测试和LICK速率测试表明，ZnD鼠标中CaCl_2的厌恶性比对照组大。通过用对照饮食喂食ZnD小鼠1周，回收了对CACL_2的行为反应的变化。结果表明，味道可能对增强ZnD鼠标的NaCl偏好不起作用，并且ZnD鼠子具有区分基本味道品质的能力。结果还表明，锌缺乏可能会改变小鼠CaCl_2的味觉引导的饮酒行为。 2.大鼠中丘巴尼神经反应的电生理学记录显示，两组之间各种味觉刺激的浓度反应关系没有显着差异，这表明Zn缺乏对味道刺激的味道对口味敏感性的影响可忽略不计。 3. ZnD鼠标中的s na水平比对照和配对大鼠的ZnD鼠标往往更低，更可变。每天的尿NA排泄量会增加ZnD鼠标。在ZnD大鼠的血清中，血管紧张素II和Ca水平显着降低，而醛固酮水平显着升高。结果表明，醛固酮上调的钠代谢障碍与锌缺乏有关。

项目成果

Zinc deficiency and salt preference.

缺锌和偏爱盐。

• 发表时间: 2006
• 期刊: Jpn J Taste Smell Res 13(1)
• 作者: Sako N;Katsukawa H;Nakashima K;Futani Y
• 通讯作者: Futani Y

Does taste affect preference for sodium in zinc deficient rats?

味觉会影响缺锌大鼠对钠的偏好吗？

• 发表时间: 2005
• 期刊: Jpn.J.Taste and Smell Res. 12(3)
• 作者: Futani;Y.;Sako;N.;Nakashima;K.;Katsukawa;H.;Tadano;M.;Yamamoto;K.;Sugimura;T.
• 通讯作者: T.

味覚受容機構における分子生物学的急展開

味觉受体机制分子生物学的快速发展

• 发表时间: 2004
• 期刊: 岐阜歯科学会雑誌 30
• 作者: 中島清人;勝川秀夫;硲 哲崇;杉村忠敬
• 通讯作者: 杉村忠敬

亜鉛欠乏はC57BLマウスの塩化カルシウムに対するリッキング応答を変化させる

缺锌改变了 C57BL 小鼠对氯化钙的舔舐反应

• 发表时间: 2005
• 期刊: 日本味と匂学会雑誌 12・3
• 作者: 中島清人;硲哲崇;麸谷嘉一;勝川秀夫
• 通讯作者: 勝川秀夫

Molecular biological progress in receptors and transduction mechanisms In taste.

味觉受体和转导机制的分子生物学进展。

• 发表时间: 2004
• 期刊: J.Gifu Dent.Soc. 30(Suppl.)
• 作者: Nakashima K;Katsukawa H;Sako N;Sugimura T
• 通讯作者: Sugimura T