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セイヨウナシのS遺伝子型同定システムの開発と単為結果性・自家摘果性の評価

梨S基因型鉴定系统的建立及单性结实和自倒能力评价

基本信息

批准号：
15780025
负责人：
高崎剛志
金额：
$ 2.37万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15780025/
关键词：
自家不和合性セイヨウナシ S遺伝子型 S-RNase PCR-RFLP

项目摘要

セイヨウナシ5品種に対して除雄無受粉と他家受粉処理を行い,単為結果果実と有種子果実の果実形質を調査した.単為結果果実の大きさ,糖度は有種子果実より劣っていたことから,ラ・フランスなどの強い単為結果性を持つ品種でも他家受粉が必要であることを明らかにした.1花そう2花の他家受粉では交配後著しい生理落果が観察された.一方1花そう1花の他家受粉は安定的な結実をもたらした.そこで,セイヨウナシ15品種に対して1花そう1花の除雄無受粉,自家受粉および他家受粉を行ない,各品種の単為結果性と自家不和合性を調査した.ほとんどすべての品種が単為結果性を有しており,結実率を指標にした不和合・和合の識別は困難であった.しかし,単為結果性の要因を排除した指標SI index[(評価対象の交配における充実種子数/交配花数)/(和合交雑における充実種子数/交配花数)×100]は和合・不和合の明確な判定を可能にした.S1〜S7対立遺伝子を有するセイヨウナシの遺伝子型を推定できるPCR-RFLP (S1〜S7)システムを用いて27品種の3遺伝子型を推定したところ,14種類の遺伝子型に分類され,そのうち8種類の遺伝子型には複数の品種が分類された.同じ推定S遺伝子型をもつ品種が交雑不和合であったことから,セイヨウナシの雌しべ側S遺伝子産物もS-RNaseであり,本システムは高い信頼性をもつことが立証された.一方で,S1〜S7-RNaseと異なる新規S8〜S11-RNaseをクローニングし,それらの全塩基配列を決定した.

5 varieties were tested except for male non-pollinated and female pollinated, and fruit quality was investigated only for fruit bearing and seed bearing. For fruit bearing, the sugar content of the seed fruit is high, and the sugar content of the seed fruit is low. For fruit bearing, the seed fruit is high, and the sugar content of the seed fruit is high. For fruit bearing, the seed fruit is high, and the seed fruit is high. For fruit bearing, the seed fruit is high. For fruit bearing, the seed fruit is high, and the sugar content of the seed fruit is high. For fruit bearing, the seed fruit is high. For fruit bearing, the seed fruit is high, and the sugar content of the seed fruit is high. For fruit bearing, the seed fruit is high, and the sugar content of the seed fruit is high. For fruit bearing, the seed fruit is high, and the seed fruit is high. For fruit bearing, the seed fruit is high. For fruit bearing, the seed fruit is high, and the seed fruit is high. For fruit bearing, the seed fruit is high. A party 1 flower 1 flower and other family powder. 15 varieties were investigated for their fruitfulness and incompatibility. It is difficult to distinguish between different varieties and results.しかし, SI index[](Comment on the number of seeds/mating flowers)/(Number of seeds/number of mating flowers)×100] Unspecific identification of uncooperative and cooperative strains is possible. PCR-RFLP (S1 ~ S7) is used to estimate the genetic types of 27 varieties and 3 genetic types of 14 species. 8 species and sub-types, and a plurality of species and classifications. The S-RNase of S-gene was identified by the same putative S-gene type, and the S-gene product of S-gene was identified by the same putative S-gene type. S1 ~ S7-RNase is different from S8 ~ S11-RNase, and the total base alignment is determined.