リハビリテーションによる脳機能回復にともなう神経回路再構成プロセスの解明

阐明与康复脑功能恢复相关的神经回路重组过程

• 批准号: 15700311
• 负责人: 肥後 範行
• 金额: $ 2.3万
• 依托单位: National Institute of Advanced Industrial Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15700311/
• 关键词: マカクザル; 皮質内微小刺激; 上肢; 器用さ; イボテン酸; 遺伝子発現; 神経成長関連タンパク; in situハイブリダイゼーション

前年度に確立した、マカクザルを用いた脳損傷後の運動機能回復を調べるための実験系を用いて、以下の研究を行った。まず、成熟脳において恒常的な変化が起きている神経回路を調べるために、シナプスの形態変化に関わる分子の発現を調べた。特に今年度は、シナプス後細胞の形態変化に関わる分子であるneurograninの、ベースラインの発現を調べた。(Higo et al.,2004)。前年度に調べた、シナプス前細胞の変化に関わる分子の変化とあわせて考えると、成熟脳においては海馬のアンモン角から海馬台にいたる投射や、大脳新皮質連合野に起始するフィードバック結合などの限られた神経回路では恒常的な神経回路変化が起きている可能性が示唆された。さらに脳損傷後の運動機能回復に伴う神経回路変化を明らかにするために、脳損傷後に精密把握の回復が見られた直後のマカクザルの脳においてシナプスの形態変化に関わる分子の発現を調べた(Murata et al.,2004)。今年度の実験では、細胞骨格アクチンフィラメントの重合を制御するシグナル伝達に関わり、神経終末の構造変化に伴って発現量が増加するタンパクであるGAP-43に着目した。精密把握の回復が見られた直後の脳において、運動関連領野を含む凍結切片を作成し、GAP-43 mRNAに対するin situハイブリダイゼーションを行った。染色の吸光度計測による定量的解析を行った結果、損傷半球の運動前野においては、非損傷半球または実験操作を加えていない個体の運動前野に比べてGAP-43 mRNAの発現の亢進が見られた。発現の亢進は運動前野の背側部よりも腹側部で顕著であった。また高倍の顕微鏡画像においてGAP-43 mRNA発現細胞の形態的特徴を検討したところ、比較的大型の錐体細胞において顕著な発現が見られた。さらに、第一次運動野の損傷領域周辺では、GAP-43の発現の低下が見られた。以上の結果から、運動前野に存在するニューロンの軸索終末で細胞骨格の再編成を伴う構造的変化が起き、これが第一次運動野損傷後の精密把握回復の構造的基盤となっている可能性が考えられる。

In the previous year, please make sure that the car is used in the previous year, and that after the use of the car, the mobile phone will be able to return to the customer. When you are mature, you are constantly changing your life. You are in the loop, you are in the shape, you are in the molecule, you are in the loop. Special attention has been paid to this year's election. After this year's election, the cell structure has been changed, the molecules have been changed, and the neurogranin has been detected. (Higo et al.,2004). In the previous year, in the previous year, in the previous year, After a long time, the mobile machine can return to the computer to realize that the information is available, and after the operation, you can make sure that you have a precise understanding of the situation, and that you can make sure that you have a real-time response, and that you can make sure that you have a real-time monitoring system, and that you can realize the Murata et al.,2004. This year, this year, the cells are in a state of coincidence. In the current year, they are responsible for the development of the industry, and in the end of the year, they are required to increase the quality of their products. This year, in the year of the year, they are responsible for the development of the industry. This year, in the year of the year, in the year of the year, this year, in the year of the year, this year, in the year of the year, in the year of this year, we need to make sure that we are responsible for the Make sure that you have a good understanding of the accuracy of the system, the field of production, including the production of in situ slices, and the accuracy of GAP-43 mRNA measurement. Quantitative analysis of dye absorbance measurements: the results of quantitative analysis, hemispheric pre-exercise field test, non-hemispheric pre-exercise operation and pre-exercise field test were significantly higher than those of GAP-43 mRNA test. It was found that the back and abdomen of the back and abdomen were suffering from hyperactivity before the exercise. High-power micro-portraits, micro-portraits, GAP-43 mRNA cells that show the shape of the cells, and large-scale cells that are compared to each other. This is the first time that the field has been affected, and that the GAP-43 has been reduced. As a result of the above results, there are some problems in the field before the exercise. The final cell bone structure is then transformed into a chemical system, and after the first exercise, there is a precise understanding of the possibility of the basic data acquisition system.

项目成果

肥後 範行: "Rehabilitative training after brain injury induced GAP-43 mRNA expression in the monkey brain : a molecular basis of motor recovery"COE Internatinal Symposium Abstract. (In press). (2004)

Noriyuki Higo：“脑损伤后的康复训练诱导猴脑中的 GAP-43 mRNA 表达：运动恢复的分子基础”COE 国际研讨会摘要（2004 年）。

肥後 範行: "Develomental changes in the distribution of MARCKS mRNA in the cerebral cortex of the macaque monkeys : molecular basis of high plasticity in the infant brain"Society for Neuroscience Abstract. 29. 459.6 (2003)

Noriyuki Higo：“猕猴大脑皮层中 MARCKS mRNA 分布的发育变化：婴儿大脑高可塑性的分子基础”神经科学学会摘要 29. 459.6 (2003)。

肥後 範行: "Cell type- and region-specific expression of neurogranin mRNA in the cerebral cortex of the macaque monkey"Cerebral Cortex. (In press). (2004)

Noriyuki Higo：“猕猴大脑皮层中神经颗粒蛋白 mRNA 的细胞类型和区域特异性表达”《大脑皮层》（正在出版）。

Development of neurogranin mRNA in the monkey cerebral neocortex.

猴大脑新皮质中神经粒蛋白 mRNA 的发育。

• 发表时间: 2004
• 期刊: Neuroscience Research Suppl50
• 作者: 肥後;範行 他
• 通讯作者: 範行 他

GAP-43 mRNA is increased in the motor cortex of the recovered brain-injured monkey.

脑损伤康复猴的运动皮层中 GAP-43 mRNA 增加。

• 发表时间: 2004
• 期刊: Neuroscience Research Suppl50
• 作者: 村田;弓 他
• 通讯作者: 弓 他