Development of evaluation system for environment through nanometric growth measurement of plant using statistical interferometry

通过统计干涉测量植物纳米生长来开发环境评估系统

• 批准号: 16310020
• 负责人: KADONO Hirofumi
• 金额: $ 8.19万
• 依托单位: Saitama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16310020/
• 关键词: Laser speckle; Statistical interferometry; Growth measurement of plant; Ozone; Environmental pollution; Photosynthetic rate; 電子的スペックル干渉

The aim of the current research is to develop an extremely sensitive system using statistical interferometry for the monitoring of the plant growth and for the evaluation of the state of environment as a final goal. Ozone was focused as a representative substance of the air pollution, and the plant growth under the ozone exposure was measured by using statistical interferometry. Experiments were also conducted for the measurement of root growth of Japanese red pine seedling with respect to the infection of ectomycorrhizas. Measurements of the plant growth with a very high temporal resolution of sub-second and extremely high accuracy of the order of sub-nanometer scale were achieved. In the experiment of ozone exposure of concentration 0.12ppm where photochemical oxidant warning is issued, the growth behavior of Chinese chives was precisely measured. It was revealed that the growth rate of the leaf was very sensitive to the ozone exposure and quickly reduces in a several seconds.As for … More the Japanese red pine, the growth rate of the roots infected with mycorrhizas showed clearly larger values in comparison with those non-infected. In addition, it was found that the roots infected with mycorrhizas became more resistive to the toxicity of ozone.So far there was no such sensitive method to measure the growth of the plant in the scale of second, some interesting phenomena were newly observed. One of the most significant phenomena is the fluctuation of the growth rate in nanometric scale. It was found that as the plant becomes more active this nanometric fluctuation becomes larger. This suggests that the nanometric fluctuation of the plant growth can be a figure to evaluate the environmental stress for the plant.In the experiment, the photosynthetic rates were also measured. However, the photosynthetic rate was almost unaffected for such a short ozone exposure of 3hours with the concentration of 0.12ppm, while the present method using statistical interferometry could detect the drastic change in growth rate of the plant leaf. Less

目前的研究的目的是开发一个非常敏感的系统，使用统计干涉测量的植物生长的监测和环境的状态作为最终目标的评估。臭氧是大气污染的代表物质，采用统计干涉法对臭氧暴露下的植物生长进行了测量。另外，还进行了外生菌根侵染对赤松幼苗根系生长影响的测定试验。实现了亚秒级的高时间分辨率和亚纳米级的极高精度的植物生长测量。在0.12ppm的臭氧暴露条件下，对韭菜的生长行为进行了精密测定。结果表明，叶片的生长速率对臭氧暴露非常敏感，在几秒钟内迅速降低， ...更多信息 日本赤松，菌根感染的根的生长速度显示明显较大的值相比，那些未感染。此外，还发现菌根侵染后的植物根系对臭氧毒害的抵抗能力增强，目前还没有一种敏感的方法可以在秒级尺度上测量植物的生长，一些有趣的现象是新观察到的。其中最显著的现象之一是纳米尺度下生长速率的波动。发现随着植物变得更加活跃，这种纳米波动变得更大。这表明，植物生长的纳米尺度波动可以作为衡量植物遭受环境胁迫的指标。然而，在0.12ppm的臭氧浓度下暴露3h，光合速率几乎不受影响，而本方法利用统计干涉法可以检测到植物叶片生长速率的急剧变化。少