Development of a Novel Evaluation Technology of Cell Attachment Ability onto Polymeric Materials Based on Quartz Crystal Microbalance (QCM) Technique

基于石英晶体微天平（QCM）技术的高分子材料细胞附着能力评价新技术的开发

• 批准号: 16360417
• 负责人: KANAMORI Toshiyuki
• 金额: $ 6.85万
• 依托单位: National Institute of Advanced Industrial Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16360417/
• 关键词: photo-responsive cell culture surface; two-dimensional cell manipulation; quartz crystal microbalance technique; elasticity change; cell-surface interaction; physicochemical interaction; 二次元培養細胞マニピュレー; 水晶振動子微小重力測定法; 光応答性材料; ポリ(N-イソプロピルアクリルアミド)

We have already developed a novel photo-responsive cell culture surface (POPS) and been successful to control the cell attachment ability of the POPS by light irradiation. In this study, we were aiming to reveal the mechanism switching the cell attachment property using quartz crystal microbalance (QCM) technique.Based on the Sauerbrey law, the change in the frequency of a quartz sensor of QCM is assumed to be proportional to the change in the weight of the sensor, so that QCM can detect extremely little adsorption onto the sensor. As the result of this study in 2004, we have found that we can evaluate the attachment and detachment of a small number of cells on the PCPS under light irradiation using QCM. In 2005, we applied the QCM technique to not only the PCPS but also several materials and have found that cell attachment on the surface of a material is dominated by physicochemical interaction between the cells and material, which occurs just after the cells approach the surface. However, it was found that the conventional QCM technique based on the Sauerbrey law is not applicable to this system, because the PCPS and cells are well-swelled elastic bodies. Then, we paid attention to QCM-D, which can detect the change in the elasticity of a material on a quartz sensor, and have had several new and valuable information about cell-surface interaction using a demonstration machine of QCM-D briefly. Now, we are preparing a manuscript about this result for publication. Incidentally, we purchased the QCM-D machine with the other budget and are proceeding with further experiments.Based on the results of this project, it has been possible for us to design PCPS much better, and we have started a new project concerning to "Development of Two-Dimensional Cell Manipulation System" with the budget of the Creation and Support Program for Start-ups from Universities in 2005 of Japan Science and Technology Agency

我们已经开发了一种新型的光响应细胞培养表面（POPS），并成功地控制光照射的POPS的细胞附着能力。本研究基于Sauerbrey定律，假设石英晶体微天平（QCM）的石英传感器的频率变化与传感器的重量变化成正比，从而QCM可以检测到传感器上极小的吸附，从而揭示了QCM技术改变细胞吸附特性的机制。作为2004年的研究结果，我们发现，我们可以使用QCM评估少量细胞在光照射下在PCPS上的附着和脱离。在2005年，我们应用QCM技术不仅PCPS，但也有几种材料，并发现，细胞附着在材料表面上的细胞和材料之间的物理化学相互作用，这发生在细胞接近表面。然而，人们发现，传统的QCM技术的基础上Sauerbrey定律是不适用于这个系统，因为PCPS和细胞是良好的溶胀弹性体。然后，我们注意到QCM-D，它可以检测材料的弹性在石英传感器上的变化，并简要地使用QCM-D演示机的细胞表面相互作用的一些新的和有价值的信息。现在，我们正在准备一份关于这一结果的手稿以供出版。顺便说一句，我们用其他预算购买了QCM-D机器，并正在进行进一步的实验。根据该项目的结果，我们有可能设计出更好的PCPS。我们已经开始了一个新的项目，关于“二维细胞操作系统的开发”与启动和支持计划的预算，日本科学技术厅2005年大学生就业情况

项目成果

Reversible and Efficient Proton Dissociation and Spirobenzopyran-Functionalized Poly(N-isopropylacrylamide) in Aqueous Solution Triggered by Light Irradiation and Temporary Temperature Rise

光照射和暂时温升引发水溶液中可逆高效质子解离和螺苯并吡喃官能化聚（N-异丙基丙烯酰胺）

• 发表时间: 2004
• 期刊: Macromolecules 37
• 作者: Sumura K.;et al.
• 通讯作者: et al.

Probing Dielectric Environment Surrounding Poly(N-isopropylacrylamide) in Aqueous Solution with Covalently Attached Spirobenzopyran

用共价键连接的螺苯并吡喃探测水溶液中聚（N-异丙基丙烯酰胺）周围的介电环境

• 发表时间: 2004
• 期刊: Langmuir 20
• 作者: Kameda M.;et al.
• 通讯作者: et al.

光応答性ゲル材料、マイクロバルブおよびマイクロチップ

光响应凝胶材料、微阀和微芯片

• 发表时间: 2005

Optimization of the sputter-deposited platinum cathode for a direct methanol fuel cell

• DOI: 10.1016/j.electacta.2005.04.062
• 发表时间: 2005-11
• 期刊: Electrochimica Acta
• 影响因子: 6.6
• 作者: Koji Makino;K. Furukawa;K. Okajima;M. Sudoh

Development of a photoresponsive cell culture surface: Regional enhancement of living-cell adhesion induced by local light irradiation

• DOI: 10.1002/app.23197
• 发表时间: 2006-04
• 期刊: Journal of Applied Polymer Science
• 影响因子: 3
• 作者: Y. Tada;K. Sumaru;Mitsuyoshi Kameda;Katsuhide Ohi;T. Takagi;T. Kanamori;Y. Yoshimi