Analysis of COMP as a Degradation Marker of Joint Matrix.

1.马关节病关节软骨低聚基质蛋白(COMP)降解与合成的分析利用与马COMP小片段亲和力较高的14G4，发现骨关节病(OA)滑液中COMP值明显高于正常人。在OASF免疫印迹上，完整的COMP所占比例明显低于正常，但碎片较小。14G4还检测到尿液中CoMP的降解。结论：1.马骨关节炎的研究中，SF和尿液中的碎片化分析可能是有用的。运动对胸主动脉血中COMP浓度的影响运动后1小时和5小时血清COMP浓度显著高于运动前水平，运动后24小时恢复至基础水平。血清COMP水平随着运动能力的提高而升高。马匹在体力应激条件下，血清基线发生显著变化。运动可促进COMP进入循环，改变COMP的基础周转率。尿液吗？用14G4单抗在免疫印迹上检测CoMP片段的阳性条带。马骨质疏松症患者尿COMP浓度明显高于正常。骨折组尿COMP与SF之间呈负对数关系，SF中尿COMP水平与基质金属蛋白酶-2和-9活性呈正相关。用单抗14G4检测尿液中的COMP有助于鉴别马的骨性关节炎。这种马的尿液中CoMP水平较高，表明其蛋白分解活性增强。

1. Analysis of cartilage oligomeric matrix protein (COMP) degradation and synthesis in equine joint disease.By use of 14G4 which had a higher affinity for the smaller fragments of equine COMP, the COMP values in synovial fluid (SF) of the osteoarthrosis (OA) were significantly higher than in the normal. On the immunoblots of OA SF, the proportions of intact COMP significantly were lower, whilst smaller fragments, compared with the normal. 14G4 also detects COMP degradation in urine. The fragmentation analysis in SF and urine could be useful to study equine osteoarthritis.2. The effect of exercise on serum concentration of COMP in ThoroughbredsSerum COMP concentration was significantly higher than baseline 1 and 5 hours after exercise and returned to baseline concentrations 24 hours after exercise. Serum baseline COMP concentration increased as the athletic ability increased. The serum baseline change significantly in response to physical stress in horses. Exercise may enhance the movement of COMP into the circulation as well as change the basal turnover rate of COMP.3. Urine COM? measurement is useful in discriminating the osteoarthritic Thoroughbreds.Positive bands of COMP fragments were determined on the immunoblots with mAb 14G4. The urinary COMP concentrations in OA horses were significantly higher than normal. In fracture cases, there was a negative logarithmic relationship between the urinary and SF COMP measurements, while the urinary COMP level was positively correlated with MMP-2 and-9 activitiy in SF. The urinary COMP assay with mAb 14G4 is useful for discriminating horses with osteoarthritis. The higher COMP levels in urine from such horses would be indicative of enhanced proteolytic activity.