New PET diagnosis of adenosine receptors in the brain, heart and skeletal muscle

本研究的目的是提出一种新的PET诊断方法，分别使用MPDX和TM SX对脑、心脏和肌肉中的腺苷A_1和A_1受体和A_1受体进行成像。通过对正常受试者应用[^&lt；11&gt；C]MPDX-PET和[^&lt；11&gt；C]TMSX-PET，利用动态PET扫描和连续动脉血数据，分别通过隔室和图形分析对A_1和A_1a&gt；受体进行了定量测定。为了避免有创的动脉采血，一种无创定量方法EPICA被成功地应用于[^&lt；11&gt；C]MPDX-和[^&lt；11&gt；C]TM SX-PET。在阿尔茨海默病患者中，在双侧内侧颞叶皮质和左侧颞顶皮质观察到MPDX结合显著降低。[^&lt；11&gt；C]MPDX的结合模式与低代谢模式不同。在颞叶癫痫患者中，[^&lt；11&gt；C]MPDX在病灶侧内侧颞叶海马区外的结合量明显减少，而在病灶侧额叶皮质的结合量增加。帕金森病患者左侧多巴胺转运体较右侧减少，双侧多巴胺D_2受体结合增加，左侧腺苷A_2受体减少。在动物实验后，对正常受试者心脏和肌肉中的腺苷受体进行了定量评估。耐力训练组男性心肌和骨骼肌中腺苷A；2A型受体的结合量高于非训练组。结论：MPDX-PET和TMX-PET可从腺苷受体的角度对各种疾病的生理和病理状态进行诊断。

The purpose of the present study is to propose new PET diagnosis using [^<11>C]MPDX and [^<11>C]TMSX for imaging adenosine A_1 and A_<2A> receptors, respectively, in the brain, heart and muscle1. By applying [^<11>C]MPDX-PET and [^<11>C]TMSX-PET to normal subjects, A_1 and A_<2A> receptors, respectively, was quantitatively measured by compartment and graphical analyses using dynamic PET scan and serial arterial blood data.2. To avoiding invasive arterial blood sampling, a non-invasive quantitative method EPICA was successfully applied to both[^<11>C]MPDX-and [^<11>C]TMSX-PET.3. In the patients with Alzheimer's disease, a significantly decreased binding of [^<11>C]MPDX was observed in the bilateral medial temporal cortex and left temporo-parietal cortex. The decreased binding pattern of [^<11>C]MPDX was different from hypometabolism. In the patients with temporal lobe epilepsy, binding of [^<11>C]MPDX was significantly decreased in the mesial temporal lobe of focus side outside the hippocampus, but increased in the frontal cortex of focus side.4. In the patients with Parkinson's disease whose the left-side dopamine transporter was more decreased than the right side one, the dopamine D_2 receptors bindings was increased bilaterally, while the adenosine A_<2A> receptor was decreased on the left side.5. Following animal experiments, adenosine A_<2A> receptors in the heart and muscle were quantitatively evaluated in normal subjects. Adenosine A_<2A> receptor binding in the cardiac and skeletal muscles was greater in the endurance-trained men than in the untrained men.We concluded that [^<11>C]MPDX-PET and [^<11>C]TMSX-PET provides diagnostic tools of the physiological and pathological states of various diseases in term of adenosine receptors.