New SEREX Antigens, Molecular Target to Diagnosis and Treatment for Esophageal Cancer

新SEREX抗原，食管癌诊断和治疗的分子靶点

• 批准号: 16390372
• 负责人: OCHIAI Takenori
• 金额: $ 9.02万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390372/
• 关键词: Esophageal cancer; Squamous cell carcinoma; SEREX; tumor marker; TROP2; GLUT-1; SLC2A1; Myomegalin; TRIM21; GLUT1; myomegalin; 食道扁平上皮癌; SEREX抗体

In order to identify new serum markers of esophageal squamous cell carcinoma (SCC), we performed serological identification of antigens by recombinant cDNA expression cloning (SEREX). E.coli was infected with λZAPII phage cDNA library prepared from mRNA of an esophageal cancer cell line (T.Tn), and IPTG-induced cDNA products were screened for interaction with antibodies in allogeneic sera of patients with esophageal SCC. We identified TROP-2, GLUT-1/SLC2A1, TRIM21, myomegalin, as new SEREX antigens for esophageal SCC. Western blotting analysis revealed that serum anti-antibodies for these SEREX antigens were present in 20 to 47% of patients but not in healthy controls. Although the presence of serum SEREX-Abs were not related to any of clinicopathological features of the patients concerned, multivariate analysis indicated that presence of s-myomegalin-Abs was significantly associated with favorable prognosis. Moreover, s-TROP-2-Abs was associated with tumor size. Because there serum antibodies against SEREX antigens were not associated with other conventional tumor markers, there serum markers were useful as a new tumor markers for esophageal SCC.

为了寻找新的食管鳞状细胞癌（SCC）血清学标志物，我们应用重组cDNA表达克隆（SEREX）技术对SCC抗原进行了血清学鉴定。以食管癌细胞株（T.Tn）的mRNA为模板制备λ ZAP Ⅱ噬菌体cDNA文库，感染大肠杆菌，筛选IPTG诱导的cDNA产物，并检测其与食管鳞癌患者异基因血清中抗体的相互作用。我们鉴定了TROP-2、GLUT-1/SLC 2A 1、TRIM 21、myomegalin为食管SCC的新SEREX抗原。Western印迹分析显示，这些SEREX抗原的血清抗抗体存在于20%至47%的患者中，但在健康对照中不存在。虽然血清SEREX-Abs的存在与患者的任何临床病理特征无关，但多因素分析表明，s-myomegalin-Abs的存在与良好的预后显著相关。此外，s-TR 0 P-2-Abs与肿瘤大小相关。由于血清中抗SEREX抗原的抗体与其他肿瘤标志物无相关性，因此这些血清标志物可作为食管鳞癌的一种新的肿瘤标志物。

项目成果

Proteomic analysis of primary esophageal squamous cell carcinoma reveals downregulation of a cell adhesion protein, periplakin

• DOI: 10.1002/pmic.200500262
• 发表时间: 2006-02-01
• 期刊: PROTEOMICS
• 影响因子: 3.4
• 作者: Nishimori, T;Tomonaga, T;Ochiai, T
• 通讯作者: Ochiai, T

TSerum anti-myomegalin antibodies in patients with esophageal squamous c ell carcinoma

食管鳞状细胞癌患者血清抗肌巨蛋白抗体

• 发表时间: 2007
• 期刊: International Journal of Oncology 30
• 作者: Shimada H.;Kuboshima M.;Shiratori T.;Nabeya Y.;Takeuchi A.;Takagi H.;Nomura;F.;Takiguchi;M.;Ochiai;T.Hiwasa
• 通讯作者: T.Hiwasa

Serological identification of TROP2 by recombinant cDNA expression cloning using sera of patients with esophageal squamous cell carcinoma

• DOI: 10.1002/ijc.20517
• 发表时间: 2004-12-20
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Nakashima, K;Shimada, H;Hiwasa, T
• 通讯作者: Hiwasa, T

Presence of serum tripartite motif-containing 21 antibodies in patients with esophageal squamous cell carcinoma

• DOI: 10.1111/j.1349-7006.2006.00192.x
• 发表时间: 2006-05-01
• 期刊: CANCER SCIENCE
• 影响因子: 5.7
• 作者: Kuboshima, M;Shimada, H;Ochiai, T
• 通讯作者: Ochiai, T

Identification of a novel SEREX antigen, SLC2A1/GLUT1, in esophageal squamous cell carcinoma.

• DOI: 10.3892/ijo.28.2.463
• 发表时间: 2006-02
• 期刊: International journal of oncology
• 影响因子: 5.2
• 作者: Mari Kuboshima;H. Shimada;Tianxiang Liu;K. Nakashima;F. Nomura;M. Takiguchi;T. Hiwasa;T. Ochiai