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The establishment of methods for cardiomyocyte differentiation with human umbilical cord blood-derived mesenchymal stem cells aimed the application into the treatment of pediatric heart failures

建立人脐带血间充质干细胞分化心肌细胞的方法，旨在应用于小儿心力衰竭的治疗

基本信息

批准号：
16390399
负责人：
AEBA Ryo
金额：
$ 11.14万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

项目摘要

The cell transplantation is a promising strategy to improve impaired organ function with injection of ex vivo cultured cells or media containing growth factors. Major advances have been made in the treatment of heart failures. However, pediatric patients suffering from heart disease is still awaiting major treatment which substitute for heart transplantation, because of several reasons such as the shortage of donor, the requirement for retransplantation, and the economic cost. Cell transplantation would have the advantage on this point, if effectiveness of therapy could be proved. Severe heart failures in childhood with variety of causes, including postoperative myocardial insufficiency, cardiovascular failure, and idiopathic dilated cardiomyopathy, are intractable and remain clinical task. Especially in Japan, it is crucial problem that cardiac transplantation in childhood has not been accepted.Thus, we focused on cardiomyocytes transplantation for pediatric patients with severe heart failure. Especially, cardiomyocytes cultured ex vivo were studied for the actual clinical application of cell transplantation. The study aims the clinical application of cell transplantation. Collected mesenchymal stem cells were analyzed for gene expression by Gene chip analysis and Western blot analysis.This study also aimed to exploit the method of cultivation or analysis of human umbilical cord blood-derived mesenchymal stem cells for safe and effective regenerative medicine. In addition, it is. necessary for cell transplantation to generate a care system for recipients in future.

细胞移植是通过注射离体培养的细胞或含有生长因子的培养基来改善受损器官功能的有前景的策略。心力衰竭的治疗已经取得了重大进展。然而，由于供体短缺、再次移植的要求和经济成本等原因，患有心脏病的儿童患者仍在等待替代心脏移植的主要治疗。细胞移植在这一点上将具有优势，如果治疗的有效性可以得到证明。儿童严重心力衰竭的病因多种多样，包括术后心肌功能不全、心血管功能衰竭和特发性扩张型心肌病，是临床上难以解决的难题。特别是在日本，儿童心脏移植尚未被接受是一个严重的问题，因此，我们将重点放在心肌细胞移植治疗严重心力衰竭的儿童患者。特别地，研究了离体培养的心肌细胞用于细胞移植的实际临床应用。本研究旨在探讨细胞移植的临床应用.本研究通过基因芯片和Western blot技术分析人脐带血间充质干细胞的基因表达情况，探索安全有效的人脐带血间充质干细胞的培养和分析方法。此外，它是。这是细胞移植所必需的，以在未来为接受者建立一个护理系统。