权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Relationship between proteins related to exocytosis regulation in salivary glands

唾液腺胞吐调节相关蛋白之间的关系

基本信息

批准号：
16390534
负责人：
SUGIYA Hiroshi
金额：
$ 8.77万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390534/
关键词：
Parotid gland Submandibular gland Primary culture Secretory granules Aquaporin cGMP Nitric oxide synthase Membrane domains 開口放出 cAMPホスホジエステラーゼ Rac シンタキシン6 Rho AQP Raft

项目摘要

In salivary glands, stimulation of β-adrenergic and muscarinic cholinergic receptors provokes exocytotic secretion of proteins. Cellular cAMP level and intracellular Ca2+ concentration are increased by activation of β-adrenergic and muscarinic cholinergic receptors, respectively, and they play as cellular signals. We investigated relationship between proteins related to exocytosis regulation and its cell signaling in salivary glands, and demonstrated several results. 1 We established a primary culture system of rat parotid acinar cells, which kept regulatory exocytotic functions provoked by β-adrenergic and muscarinic cholinergic receptor activation. 2)We confirmed the presence of aquaporirn-5 water channels in secretory granules of the rat parotid gland, and suggested that the water channels are involved in granule osmoregulation related to Cl- transport. 3)We demonstrated that the activity of nitric oxide synthase, which is activated by muscarinic receptor activation via increase in intracellular Ca2+, was regulated by phospholipids such as phosphatidyl inositol 4,5-bisphosphate and phosphachidic acid in the rabbit submandibular gland. 4)We demonstrated that cyclic nucleotide phosphodiesterases 1 and 2 were involved in the regulation of cellular cGMP level induced by muscarinic receptor activation in rabbit submandibular gland cells. 5)We found that two kinds of Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase, which were activated by Ca2+ levels induced by muscarinic receptor activation, in rabbit parotid acinar cells. 6)We suggested the relationship between changes of membrane domain (detergent-resistant membrane domains) and syntaxin 6, which is considered to be related to maturation of secretory granules. We believe that these results are new information on salivary gland function.

在唾液腺中，刺激β-肾上腺素能和毒碱能胆碱能受体刺激蛋白质的胞吐分泌。细胞内cAMP水平和细胞内钙离子浓度分别通过激活β-肾上腺素能和M胆碱能受体而升高，它们作为细胞信号发挥作用。我们研究了唾液腺中与胞吐调节相关的蛋白质与其细胞信号之间的关系，并证明了几个结果。1建立了大鼠腮腺腺泡细胞的原代培养体系，该体系能维持β-肾上腺素能和M胆碱能受体激活所引起的调节胞吐功能。2)我们证实了大鼠腮腺分泌颗粒中存在水通道，推测水通道参与了与氯离子转运有关的颗粒渗透调节。3)在兔颌下腺中，一氧化氮合酶的活性受磷脂酰肌醇、4，5-二磷酸和磷甲酸等磷脂的调节，该酶由M受体激活，通过细胞内钙的增加而激活。4)我们证实环核苷酸磷酸二酯酶1和2参与了M受体激活诱导的兔颌下腺细胞cGMP水平的调节。5)我们在兔腮腺腺泡细胞中发现了两种钙/钙调蛋白依赖性的环核苷酸磷酸二酯酶，这两种环核苷酸磷酸二酯酶是由M受体激活引起的钙离子水平所激活的。6)我们提出了膜结构域(抗洗涤剂膜结构域)的变化与Synaxin 6之间的关系，认为这与分泌颗粒的成熟有关。我们认为这些结果是关于唾液腺功能的新信息。