REGULATION OF WATER TRANSPORT VIA PARACELLULAR PATHWAY BY PHOSPHORYLATION AND DEPHOSPHORYLATION IN SALIVARY GLASNDS

唾液腺中磷酸化和去磷酸化对细胞旁途径水运输的调节

• 批准号: 21592375
• 负责人: SUGIYA Hiroshi
• 金额: $ 2.83万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592375/
• 关键词: 顎下腺; 水分泌; 傍細胞輸送; カルシウムイオン; タイト結合; 翻訳後修飾; ニューロキニンA; IGF-I; ピロカルピン; 細胞内カルシウム; クローディン; PI3キナーゼ

The relationship between neurokinin A-or pilocarpine-provoked salivary fluid secretion and paracellular transport by using Lucifer Yellow was investigated in perfused rat submandibular gland. Salivary fluid secretion provoked by neurokinin A-or pilocarpine was dependent on the increase in intracellular Ca^<2+> concentrations, which was linked to Lucifer Yellow secretion. These results suggest that Ca^<2+>-dependent phosphorylation and/or dephosphorylation appears to contribute to the paracellular transport. On the other hand, expression and localization of tight junction proteins was induced by insulin-like growth factor I(IGF-I) in cultured rat submandibular gland cells, suggesting that IGF-I contributes to maintenance of the paracellular pathway system in salivary gland. Since the IGF-I effect was inhibited by PI3-kinase inhibitors, phosphorylation by PI3-kinase appears to contribute to the effect of IGF-I.

在大鼠颌下腺灌流模型上，用荧光黄研究了神经激动素A或匹罗卡品引起的唾液分泌与细胞旁转运的关系。神经激动素A或匹罗卡品引起的唾液分泌依赖于细胞内钙离子浓度的增加，这与荧光黄的分泌有关。这些结果表明，钙离子依赖的磷酸化和/或去磷酸化似乎有助于细胞旁运输。另一方面，胰岛素样生长因子I(IGF-I)诱导培养的大鼠颌下腺细胞表达和定位紧密连接蛋白，提示IGF-I参与了唾液腺细胞旁通路系统的维持。由于IGF-I的作用被PI3-激酶抑制剂抑制，因此PI3-激酶的磷酸化似乎有助于IGF-I的作用。

项目成果

Maintenance of paracellular barrier function by insulin-like growth factor-I in submandibular gland cells

• DOI: 10.1016/j.archoralbio.2010.07.023
• 发表时间: 2010-12-01
• 期刊: ARCHIVES OF ORAL BIOLOGY
• 影响因子: 3
• 作者: Mitsui, Retsu;Fujita-Yoshigaki, Junko;Sugiya, Hiroshi
• 通讯作者: Sugiya, Hiroshi

cAMP-dependent exocytosis in parotid acinar cells

腮腺腺泡细胞中 cAMP 依赖性胞吐作用

• 发表时间: 2011
• 作者: Ishii H;Niioka T;Izumi H;Kakuguchi Wataru;Sugiya H
• 通讯作者: Sugiya H

ヒト歯髄培養細胞におけるエンドサイトーシスを介したMMP-3によるCTGF/CCN2発現調節

MMP-3 通过胞吞作用调节人牙髓细胞中的 CTGF/CCN2 表达

• 发表时间: 2011
• 作者: 室町幸一郎;神尾直人;成田貴則;神尾素代;杉谷博士;松島潔
• 通讯作者: 松島潔

Parotid acinar cells transiently change to duct-like cells during epithelial-mesenchymal transition

• DOI: 10.2152/jmi.56.258
• 发表时间: 2009-12-01
• 期刊: JOURNAL OF MEDICAL INVESTIGATION
• 影响因子: 0.7
• 作者: Fujita-Yoshigaki, Junko;Qi, Bing;Sugiya, Hiroshi
• 通讯作者: Sugiya, Hiroshi

Separation of immature granules containing color dye from the rat parotid gland

从大鼠腮腺中分离含有彩色染料的未成熟颗粒

• 发表时间: 2009
• 作者: Osamu Katsumata-Kato;Junko Fujita-Yoshigaki;Keitaro Satoh;Takanori Narita;Hiroshi Sugiya
• 通讯作者: Hiroshi Sugiya