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Research of Development of Restorative Material Using Enamel regenerated and CAD/CAM

再生牙釉质及CAD/CAM修复材料的开发研究

基本信息

批准号：
16390560
负责人：
TERADA Yoshihiro
金额：
$ 8.7万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

In the field of dental medicine, improvement of Quality of Life (QOL) is required because tooth loss due to periodontal disease and dental caries with progression of aging continues to affect most elderly at some time in their lives. Therefore, it is essential for occlusal maintenance, oral hygiene and esthetic restorations at the highest levels to pursue the goal of treatment at dentistry. Application of tooth regeneration using tissue engineering concepts for convemtional dentistry is a promising biological approach to solving problems of tooth loss in elderly patients in prosthodontics.We aimed at dental enamel regeneration using tissue engineering capable of clinical application. In the present study, we investigated the cultivation of odontogenic epithelial cells derived from porcine tooth buds and the reproduction of enamel bulk by stratification of these cell layers. Epithelial cells isolated from porcine tooth buds of third molar can be subcultured by 2 passages and expressed a … More melogenin which is specific for dental enamel, indicating that these cells have the characters of ameloblast progenitor.Moreover the isolated odontogenic epithelial cells were seeded onto biodegradable polyglycolic acid (PGA) fiber membranes and cultured in D-MEM containing 10% fetal bovine serum for a week. After culturing, membranes were observed by the scanning electron microscopy (SEM) and cell nuclear staining using fluorescent microscopy (EM). The result showed that epithelial cells were spread uniformly and colonilized on PGA membrane. Following that, ten membranes where epithelial cells colonilized were piled up and implanted into the omenta of the athymic rats. At 4 weeks after implantation, samples developed were dissected, prepared tissue sections and stained with hematoxylin and eosin (H-E). Regenerated tissues at inter-membrane were confirmed by observation of tissue sections by light microscopy.In conclusion, our findings imply that tissue regeneration was achieved by the stratification of cultured odontogenic epithelial cells using biodegradable PGA membranes. Further comprehensive studies will be necessary to clarify the sequence of differentiation events and the cell lineage of enamel tissue regeneration for clinical application. Less

在牙科医学领域，需要提高生活质量（QOL），因为随着年龄的增长，由于牙周病和龋齿导致的牙齿脱落会在生活中的某个时候继续影响大多数老年人。因此，它是必不可少的咬合保持，口腔卫生和美学的最高水平，追求的目标，在牙科治疗。将组织工程学的概念应用于牙齿再生是解决口腔修复中老年人牙齿缺失问题的一种很有前途的生物学方法。本研究以猪牙胚为材料，对牙源性上皮细胞的体外培养及分层培养的方法进行了研究。从猪第三磨牙牙胚中分离的上皮细胞可传代培养2代，并表达 ...更多信息将分离的牙源性上皮细胞接种于可生物降解的聚乙醇酸（polyglycolic acid，PGA）纤维膜上，在含10%胎牛血清的D-MEM中培养1周。培养后，通过扫描电子显微镜（SEM）和使用荧光显微镜（EM）的细胞核染色观察膜。结果表明，上皮细胞在PGA膜上均匀分布并定殖。随后，将10个上皮细胞定殖的膜堆积并植入无胸腺大鼠的网膜中。在植入后4周，解剖发育的样品，制备组织切片并用苏木精和伊红（H-E）染色。通过光学显微镜观察组织切片证实了膜间再生组织。总之，我们的研究结果表明，组织再生是通过使用可生物降解的PGA膜对培养的牙源性上皮细胞进行分层来实现的。进一步的全面研究将是必要的，以澄清分化事件的顺序和细胞谱系的釉组织再生的临床应用。少