遺伝的感受性を考慮した慢性C型肝炎の進展を予防する生活習慣に関する分子疫学的研究

考虑遗传易感性的生活方式习惯预防慢性丙型肝炎进展的分子流行病学研究

• 批准号: 16790325
• 负责人: 松永 めぐみ
• 金额: $ 2.24万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16790325/
• 关键词: 肝臓がん; C型肝炎; 食物摂取頻度; 遺伝子多型; 生活習慣; 肝臓癌

【背景】肝炎の進展には酸化ストレスの関与が示唆され、喫煙や飲酒、肥満などの生活習慣や、薬物代謝酵素やサイトカインの遺伝子多型などの遺伝的感受性要因が報告されているが、肝炎進展を抑制する食品や栄養素、それらと遺伝的感受性要因との相互作用についての知見は十分でない。【目的】最終年度にあたり、慢性C型肝炎の進展に関与する生活習慣と遺伝子多型との関連を検討する。【方法】症例(肝癌)対照(肝硬変、慢性肝炎)研究の手法で、研究参加への同意を得た40〜79歳の慢性C型肝炎患者272人、肝硬変患者60人、肝癌患者87人に自記式の半定量食物摂取頻度調査票を用いた調査を行った。(平成17年度)。平成18年度は、同集団から採取したバッフィーコートよりDNAを抽出後、薬物代謝酵素などの遺伝子多型の測定をすすめた。測定方法は、Polymerase chain reactionにより多型部位を増幅し、制限酵素断端長多型などを用いて決定した。生活習慣と遺伝子多型の肝癌との関連をlogistic regression modelで検討した。また、本年度は調査施設を新たに加え慢性肝炎患者119人についても調査を終了した。【結果】飲酒(オッズ比(OR):1.8,95%信頼区間(CI):1.0-3.0)と喫煙(OR:2.5,95%CI:1.4-4.5)は肝癌と有意な正の関連を示した。遺伝子多型と飲酒に関しては、ALDH2の*1/*1型に比べ*2/*1型では、1日3合未満の中等量の飲酒により肝癌のオッズ比上昇が見られた(OR:1.8,95%CI:0.8-3.8)。遺伝子多型と喫煙の関連ではCYPIA2の野生型でかつ喫煙者では肝癌リスクの有意な上昇(OR:4.5,95%CI:2.2-8.9)が見られた。【考察】肝癌の最大の危険因子はC型肝炎ウイルスの感染であるが、本研究では慢性肝疾患患者を対照群に設定することで影響を除外できた。肝癌の危険因子として飲酒と喫煙があるが、薬物代謝酵素の遺伝子多型と交互作用のもとで肝癌の発生に寄与する可能性が示唆された。今後は、各種遺伝子多型の判定を進め、遺伝的感受性要因と食事由来の因子と汗顔の関連についても検討していく予定である。

[Background] The progression of hepatitis is related to the acidification of hepatitis and the symptoms of hepatitis, including smoking and drinking. Fertilizer metabolism enzyme やサイトカインの缝子multitype などのThe susceptibility factor of the disease is the report, the progress of hepatitis is suppressed, the food is nutritious, the susceptibility factor of the disease is the interaction of the disease, the knowledge is very good. [Purpose] The relationship between chronic hepatitis C and chronic hepatitis C progression in the final year is related to lifestyle habits and multi-type disease. [Methods] Case study (liver cancer) (liver cirrhosis, chronic hepatitis) study method, consent for study participation, chronic hepatitis C between 40 and 79 years old 272 patients with inflammatory disease, 60 patients with liver cirrhosis, and 87 patients with liver cancer were surveyed using self-recorded semi-quantitative food frequency questionnaires. (2017). In 2018, the same collection was conducted to extract the DNA from the したバッフィーコートより, and to measure the polytype of the enzyme metabolizing enzyme などの缝子. Determination method, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction, Polymerase chain reaction. Lifestyle habits and multiple types of liver cancer are related to each other using a logistic regression model. This year, the investigation of 119 patients with chronic hepatitis in new facilities was completed this year. [Results] Drinking alcohol (OR: 1.8, 95% confidence interval (CI): 1.0-3.0) and smoking (OR: 2.5, 95% CI: 1.4-4.5) are significantly associated with liver cancer. There are multiple types of alcohol consumption, including alcohol consumption, ALDH2 type *1/*1 type, ratio of *2/*1 type, and 3 combinations per day. Moderate drinking was associated with an increased risk of liver cancer (OR: 1.8, 95% CI: 0.8-3.8). There is an association between polymorphism and smoking, and there is a significant increase in liver cancer in smokers (OR: 4.5, 95% CI: 2.2-8.9). [Investigation] The biggest risk factor for liver cancer is hepatitis C and infection. In this study, patients with chronic liver diseases were excluded from the group setting and the impact was not affected. Risk factors for liver cancer include drinking alcohol and eating cigarettes, and the polymorphism of liver metabolizing enzymes and the interaction between liver cancer and the possibility of liver cancer are indicated. From now on, the determination of various types of posthumous syndrome and the susceptibility of the postmortem will be determined based on factors such as the origin of food and the relationship between shame and shame.