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Human kallikrein subtypes produced by gynecplogical cancer

妇科癌症产生的人激肽释放酶亚型

基本信息

批准号：
16591678
负责人：
MIYAGI Etsuko
金额：
$ 1.7万
依托单位：
Yokohama City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16591678/
关键词：
ovarian cancer adenocarinoma kallikrein serine protease clear cell adenocarcinoma serous adenocarcinoma tissue tvne gene expression

项目摘要

【Objectives】 Some kinds of serine proteases are known to play important roles in the process of malignant progression of cancer cells. In addition, prostate specific antigen (PSA) , which is identical to human kallikrein 3, has been an effective biomarker for prostate cancer. However, little is known about the role of kallikrein (KLK) subtypes, which have activities like trypsin and chymotrypsin in the progression of ovarian cancer.【Methods】Gene expression of KLK subtypes 4,5,6,9,10 and 11 were examined quantitatively using RT-PCR methods in both ovarian cancer cell lines and tissues.【Results】 (1) In 9 ovarian cancer cell lines and 28 ovarian cancer tissues, KLK 10 gene was revealed to be expressed frequently(cell lines 89%, tissues 100%) and to a high degree. (2) KLK 5 had a tendency to be expressed highly in serous and endometrioid adenocarcinoma tissues compared to clear and mucinous adenocarcinoma. (3) Clear cell adenocarcinoma showed high degree KLK 6 expression and low degree KLK5, 11. In serous adenocarcinoma tissues, KLK 11 was highly expressed compared to the other tissue types. (4) There was no correlation between the gene expression of KLK subtypes and clinical stages or serum CAl25 levels.【 Conclusions】 It was revealed that ovarian cancer cells express various KLK subtypes. KLK 10 was found to have possibility as a new biomarker of ovarian adenocarcinoma considering very high frequent and high degree gene expression. In addition, various expression patterns of KLK subtypes may relate to specific clinical characteristics in some tissue types of ovarian cancer.

[目的]已知某些丝氨酸蛋白酶在癌细胞恶性进展过程中起重要作用。此外，前列腺特异性抗原（PSA），这是相同的人激肽释放酶3，已成为前列腺癌的有效生物标志物。然而，关于激肽释放酶（KLK）亚型的作用知之甚少，KLK亚型在卵巢癌的进展中具有胰蛋白酶和糜蛋白酶等活性。[方法]采用RT-PCR方法检测卵巢癌细胞株及卵巢癌组织中KLK亚型4、5、6、9、10和11的基因表达。[结果]（1）KLK 10基因在9株卵巢癌细胞系和28例卵巢癌组织中呈高表达（细胞系89%，组织100%）。(2)KLK 5在浆液性腺癌和类浆液性腺癌组织中的表达明显高于透明性腺癌和粘液性腺癌。(3)透明细胞腺癌显示高度KLK 6表达和低度KLK 5，11。在浆液性腺癌组织中，与其他组织类型相比，KLK 11高表达。(4)KLK亚型基因表达与临床分期及血清CA 125水平无相关性。[结论]卵巢癌细胞表达多种KLK亚型。KLK 10基因表达频率高，表达程度高，有可能作为卵巢腺癌的新生物标志物。此外，KLK亚型的各种表达模式可能与某些组织类型卵巢癌的特定临床特征有关。