Antitumor effect of gene transfection and DNA vacccine of beta-defensins

β-防御素基因转染及DNA疫苗的抗肿瘤作用

• 批准号: 16591894
• 负责人: ABIKO Yoshihiro
• 金额: $ 2.08万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16591894/
• 关键词: Beta-defensin; Antitumor; Gene therapy; DNA vaccine; Squamous cell carcinoma; Lymphoma; 樹状細胞; 遺伝子導入; CCR6

The present study examined whether increased expression of beta-defensins by the gene transfection affected antitumor of squamous cell carcinoma (SCC), and whether DNA vaccine with beta-defensins affected antitumor of lymphoma. In order to observe the antitumor effect of increased expression of beta-defensins on SCC, adenovirus vectors with beta-defensin were transfected into SCC tissues in nude mice. The diameter of the tumor mass into which the gene was trandected was significantly smaller than that of the controls. The results indicate that beta-defensins themselves may posses a direct antitumor effect against SCC. In order to observe the antitumor effect of DNA vaccine with beta-defensins on lymphoma, the vaccine was injected into A20 lymphoma inBALB/c mice. The survival rate of the vaccination group was significantly longer than that of the control group. The results indicate that DNA vaccine with beta-defensins may be useful in the treatment of lymphoma.

本研究探讨了通过基因转染提高β -防御素的表达是否影响鳞状细胞癌（SCC）的抗肿瘤作用，以及β -防御素DNA疫苗是否影响淋巴瘤的抗肿瘤作用。为了观察β -防御素表达增加对鳞状细胞癌的抗肿瘤作用，我们将β -防御素腺病毒载体转染裸鼠鳞状细胞癌组织。基因转染后的肿瘤肿块直径明显小于对照组。结果表明β -防御素本身可能对SCC具有直接的抗肿瘤作用。为了观察β -防御素DNA疫苗对淋巴瘤的抗肿瘤作用，将该疫苗注射到balb /c小鼠A20淋巴瘤中。接种组的存活率明显长于对照组。结果表明，含有β -防御素的DNA疫苗可用于淋巴瘤的治疗。

项目成果

Single-nucleotide Polymorphism(SNP) in 6-Defensin 2 in a Japanese Population and an Effect of - 1029 SNP on Promoter Activity

日本人群6-防御素2单核苷酸多态性(SNP)及-1029 SNP对启动子活性的影响

• 发表时间: 2005
• 期刊: Oral Science International 2
• 作者: Kusano;k.;Abiko;Y.;Nishimura;M.;Arakawa;T.;Takeshima;M.;Fujimoto;A.;Takuma;T.;Kaku;T
• 通讯作者: T

Beta defensin-s engineered epidermis shows highly protective effect fro bacterial infection

Beta 防御素工程化表皮对细菌感染表现出高度保护作用

• 发表时间: 2005
• 期刊: Gene Therapy 12
• 作者: Sawamura D;Goto M;Shibaki A;Akiyama M;McMillan JR;Abiko Y;Shimizu H.
• 通讯作者: Shimizu H.

Connexin 43 transfection in basaloid squamous cell carcinoma cells.

• DOI: 10.3892/or.16.2.285
• 发表时间: 2006-08
• 期刊: Oncology reports
• 影响因子: 4.2
• 作者: K. Shima;T. Muramatsu;Y. Abiko;Y. Yamaoka;Hodaka Sasaki;M. Shimono

Increased expression of beta-defensin-2 and -3 during the development of autoimmune sialoadenitis in MRL/lpr mice. Med Mol Morph

MRL/lpr 小鼠自身免疫性唾液腺炎发展过程中 β-防御素-2 和 -3 的表达增加。

• 发表时间: 2007
• 期刊: Med Mol Morph 40
• 作者: Saitoh;M.;Kurashige;Y.;Yamazaki;M.;Nishimura;M.;Nakamura;S.;Noro;D.;Takeshima;M.;Arakawa;T.;Takuma;T.;Igarashi;S.;Kaku;T.;Inoue;T.;Abiko;Y
• 通讯作者: Y

口腔扁平苔癬におけるβディフェンシン2の発現と臨床病理学的所見との関連についての検討

口腔扁平苔藓β-防御素2表达与临床病理关系的探讨

• 发表时间: 2004
• 期刊: 目口粘膜誌 10(1)
• 作者: 野口忠秀;神部芳則;草間幹夫;安彦善裕;賀来 亨
• 通讯作者: 賀来 亨