Basic study on optical coherence tomography to analyze neuro-activity

光学相干断层扫描分析神经活动的基础研究

• 批准号: 17300140
• 负责人: SATO Manabu
• 金额: $ 9.87万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300140/
• 关键词: Low Coherence; Full Feild-OCT; Quadrature Phase; Optical Hilbert Transform; Video-Rate; Rat Brain

The objective of this research is confirmations that quadrature fringes full field optical coherence tomography (QF IF OCT) proposed by us is valuable to study on neuro-activities in cortexes. It was impossible to obtain the en-face OCT images to show the neuro-activity in rat cortex. However we could notice the difference in depth intensity profiles through the comparisons between in vivo and in situ.The measured three-dimensional (3D) area was 4 x 4 x 2.9 mm^3 (160x160x400pixels, XxYxdepth). In signal variations of OCT image on time, the mean of signal intensity in situ was 1.4 times larger than that of in vivo. The ratio of standard deviation to mean of signal intensity in vivo was 2 times larger than that of in situ. In vivo 3D imaging, the cross-section of thinned skull with thickness of 170 μm and the vein with of 150 μm were recognized without averaging. The vein with diameter of 70 μm was also recognized by averaging 300 en face images. In situ 3D imaging, cross-sections of the thinned skull and the vein with a diameter of 200 μm were measured. Compared the depth intensity profile in situ with in vivo, in case of in situ the maximum was observed below the surface at the depth of 1500 μm. This difference in intensity profiles between in vivo and in situ might be useful to measure and monitor the viability of cortexes non-invasively with time and spatial resolution.

这项研究的目的是确认我们提出的正交边缘的全场光学相干断层扫描（如果OCT）是我们提出的，对于研究皮质中的神经活性很有价值。不可能获得欧元oct图像以显示大鼠皮层中的神经活性。但是，我们可以通过体内和原位的比较注意到深度强度曲线的差异。测得的三维（3D）面积为4 x 4 x 2.9 mm^3（160x160x400pixels，xxyxdepth）。在OCT图像的信号变化时，信号强度原位的平均值比体内的平均值高1.4倍。标准偏差与体内信号强度平均值的比率比原位大2倍。在体内3D成像中，厚度为170μm的稀薄颅骨的横截面和150μm的静脉被识别而无需平均。直径为70μm的静脉也通过平均300个脸部图像识别。原位3D成像，测量直径为200μm的颅骨和静脉的横截面。将原位的深度强度曲线与体内的原位进行了比较，如果原位，则在1500μm的深度下在表面下方观察到最大值。体内和原位之间强度曲线上的这种差异对于通过时间和空间分辨率非侵入性地测量和监测皮质的生存能力可能是有用的。

项目成果

Quadrature fringes wide-field optical coherence tomography and its applications to biological tissues

• DOI: 10.1016/j.optcom.2006.10.029
• 发表时间: 2007-03
• 期刊: Optics Communications
• 影响因子: 2.4
• 作者: M. Sato;T. Nagata;T. Niizuma;L. Neagu;R. Dabu;Y. Watanabe

直交位相干渉フルフィールドOCT

正交相位干涉全场OCT

• 发表时间: 2006
• 作者: Koizumi;S.;Fujishita;K.;Inoue;K.;佐藤学・渡部裕輝
• 通讯作者: 佐藤学・渡部裕輝

In Situ Rat Brain Imaging using Full field OCT

使用全视野 OCT 进行原位大鼠脑成像

• 发表时间: 2005
• 作者: Nagata Tetsuhiro;Yasuhisa Gojou;Yuuki Watanabe;Takashi Yamaguchi;Tsutomu Ichimura and Manabu Sato
• 通讯作者: Tsutomu Ichimura and Manabu Sato

Quadrature Fringe Full-field OCT

正交条纹全视野 OCT

• 发表时间: 2006
• 作者: Fujishita;K.;Ozawa;T.;Shibata;K.;Tanabe;S.;Sato;Y.;Hisamoto;M.;Okuda;T.;Koizumi. S.;Manabu Sato and Yuuki Watanabe
• 通讯作者: Manabu Sato and Yuuki Watanabe

High-speed time domain optical coherence tomograpy

高速时域光学相干断层扫描

• 发表时间: 2007
• 作者: Shinozaki;Y.;Koizumi.S.;Ishida;S.;Sawada;J.;Ohno;Y.;Inoue;K.;Manabu Sato and Yuuki Watanabe
• 通讯作者: Manabu Sato and Yuuki Watanabe