Steroidogenesis in the skeletal muscle

骨骼肌中的类固醇生成

• 批准号: 17300204
• 负责人: MESAKI Noboru
• 金额: $ 10.46万
• 依托单位: Teikyo Heisei University (2007)University of Tsukuba (2005-2006)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300204/
• 关键词: skeletal muscle; sex hormone; autocrine; exercise; aging; トレーニング; 自己産生

Sex steroid hormones, such as testosterone and estradiol, play important roles in developing both strength and mass of skeletal muscle. Circulating dehydroepiandrosterone (DHEA)is peripherally taken up and converted to testosterone by 3B-hydroxysteroid dehydrogenase (HSD) and 17B-HSD, and testosterone, in turn, is irreversibly converted to estrogens by aromatase cytochrome P450 (P450arom). Although sex steroid hormones have been implicated in skeletal muscle regulation and adaptation, it is unclear whether skeletal muscles have a local steroidogenic enzymatic machinery capable of metabolizing circulating DHEA. First study, we investigate whether the three key steroidogenic enzymes are present in the skeletal muscle, and are capable of generating sex steroid hormones. These findings are novel and important in that they provide the first evidence showing that skeletal muscles are capable of locally synthesizing sex steroid hormones from circulating DHEA or testosterone. Second study, we examine sex differences in the levels of testosterone, estradiol, and steroidogenesis-related enzymes, such as 3B-HSD, 17B-HSD and P450arom, in the skeletal muscle at rest and after exercise. We studied the gastrocnemius muscles of resting rats(10 weeks old)and exercised rats(10 weeks old, treadmill running, 30 m/min, 30 min). A single bout of exercise can influence the steroidogenic system in skeletal muscle and these alterations differ between sexes. The acute exercise-induced alteration of steroidogenic enzymes may enhance the local steroidogenesis in the skeletal muscle in both sexes.

性类固醇激素，如睾酮和雌二醇，在骨骼肌力量和质量的发展中起着重要作用。循环脱氢表雄酮（DHEA）被外周吸收并通过3B-羟基类固醇脱氢酶（HSD）和17 B-HSD转化为睾酮，而睾酮又通过芳香酶细胞色素P450（P450 arom）不可逆地转化为雌激素。虽然性类固醇激素与骨骼肌的调节和适应有关，但目前尚不清楚骨骼肌是否具有能够代谢循环DHEA的局部类固醇生成酶机制。首先，我们研究骨骼肌中是否存在三种关键的类固醇生成酶，并能够产生性类固醇激素。这些发现是新颖的和重要的，因为它们提供了第一个证据表明骨骼肌能够从循环DHEA或睾酮局部合成性类固醇激素。第二项研究，我们研究了在休息和运动后骨骼肌中睾酮，雌二醇和类固醇生成相关酶，如3B-HSD，17 B-HSD和P450 arom水平的性别差异。我们研究了安静大鼠（10周龄）和运动大鼠（10周龄，跑台运动，30 m/min，30 min）的腓肠肌。一次运动可以影响骨骼肌中的类固醇生成系统，这些变化在性别之间存在差异。急性运动引起的类固醇生成酶的改变可能会增强骨骼肌中局部类固醇的生成。

项目成果

Expression of steroidogenic enzymes and synthesis of sex steroid hormones from DHEA in skeletal muscle of rats

• DOI: 10.1152/ajpendo.00367.2006
• 发表时间: 2007-02-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
• 影响因子: 5.1
• 作者: Aizawa, Katsuji;Iemitsu, Motoyuki;Mesaki, Noboru
• 通讯作者: Mesaki, Noboru

運動による新たな性ホルモン合成経路

运动诱导新的性激素合成途径

• 发表时间: 2007
• 作者: T.Asai;H.Nunome;Y.Kamata R.Sakashita;相澤 勝治
• 通讯作者: 相澤 勝治

Acute exercise alters expression of steroidogenesis-related enzymes in the skeletal muscle of rats

急性运动改变大鼠骨骼肌中类固醇生成相关酶的表达

• 发表时间: 2006
• 作者: Katsuji Aizawa;Seiji Maeda;Motoyuki Iemitsu;Takeshi Otsuki;Saburina Jesmin;Takashi Miyauchi;Noboru Mesaki
• 通讯作者: Noboru Mesaki

運動は骨格筋局所の性ステロイドホルモン代謝関連酵素を活性化させる

运动激活骨骼肌局部与性类固醇激素代谢相关的酶

• 发表时间: 2005
• 作者: 相澤 勝治;前田 清司;家光 素行;大槻 毅;宮内 卓;目 崎登
• 通讯作者: 目 崎登

一過性運動による骨格筋の性ホルモン合成酵素の発現と性差

骨骼肌中性激素合酶的表达以及短暂运动引起的性别差异

• 发表时间: 2006
• 作者: 相澤 勝治;家光 素行;前田 清司;大槻 毅;宮内 卓;目崎 登
• 通讯作者: 目崎 登