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Plant signal transduction and gene expression in response to water stress condition

植物响应水分胁迫的信号转导和基因表达

基本信息

批准号：
17370014
负责人：
SHINOZAKI Kazuko
金额：
$ 9.34万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17370014/
关键词：
water stress transcriptional legulation promoter analysis 水ストレス

项目摘要

Transcription factors DREB1A/CBF3 and DREB2A interact with a cis-acting element, DRE, involved in cold- and drought-stress-responsive gene expression in Arabidopsis. Intact DREB2A expression does not activate downstream genes under normal growth conditions, suggesting that DREB2A requires post-translational modification for activation. DREB2A domain analysis using protoplasts identified a transcriptional activation domain between residues 254 and 335, and deletion of a region between residues 136 and 165 transforms DREB2A to a constitutive active form. Overexpression of constitutive active DREB2A resulted in significant drought stress tolerance but only slight freezing tolerance in transgenic plants. Microarray revealed that DREB2A regulates expression of many water-stress-inducible genes. However, some genes downstream of DREB2A are not downstream of DREB1A, which also recognizes DRE but functions in cold-stress-responsive gene expression. Green fluorescent protein gave a strong signa … More l in the nucleus under unstressed control conditions when fused to constitutive active DREB2A but only a weak signal when fused to full-length DREB2A. The region between DREB2A residues 136 and 165 plays a role in the stability of this protein in the nucleus, which is important for protein activation.Microarray analysis of transgenics overexpressing DREB2A CA also indicated that the overexpression of DREB2A CA induces heat-shock (HS) related genes. Moreover, we found that transient induction of the DREB2A occurs rapidly by HS stress and that the sGFP-DREB2A protein accumulates in nuclei of HS stressed cells. DREB2A upregulated genes were classified into 3 groups based on their expression patterns : genes induced by HS, genes induced by drought stress and genes induced by both HS and drought stress. DREB2A upregulated genes were downregulated in DREB2A knockout mutants under stress conditions. Thermotolerance was significantly increased in the plants overexpressing DREB2A CA and decreased in the DREB2A knockout plants. Collectively, these results indicate that DREB2A functions in both water and HS stress responses. Less

转录因子DREB 1A/CBF 3和DREB 2A与一个顺式作用元件DRE相互作用，参与拟南芥中冷和干旱胁迫响应基因的表达。完整的DREB 2A表达在正常生长条件下不激活下游基因，这表明DREB 2A需要翻译后修饰才能激活。使用原生质体的DREB 2A结构域分析鉴定了残基254和335之间的转录激活结构域，并且残基136和165之间的区域的缺失将DREB 2A转化为组成型活性形式。组成型活性DREB 2A的过表达导致显着的干旱胁迫耐受性，但只有轻微的抗冻性在转基因植物。基因芯片显示DREB 2A调控许多水分胁迫诱导基因的表达。然而，DREB 2A下游的一些基因不是DREB 1A的下游，DREB 1A也识别DRE，但在冷胁迫响应基因表达中起作用。绿色荧光蛋白在细胞内表达， ...更多信息 1在非应激对照条件下，当与组成型活性DREB 2A融合时，在细胞核中表达，但当与全长DREB 2A融合时，仅表达弱信号。DREB 2A的136 - 165位残基之间的区域对DREB 2A在细胞核中的稳定性起着重要的作用，这对于蛋白质的活化是重要的。此外，我们发现，瞬时诱导的DREB 2A发生迅速HS应激和sGFP-DREB 2A蛋白积累在HS应激细胞的细胞核中。DREB 2A上调基因根据其表达模式可分为3类：HS诱导基因、干旱胁迫诱导基因和HS和干旱胁迫共同诱导基因。DREB 2A基因敲除突变体在胁迫条件下表达下调。耐热性显着增加的植物过表达DREB 2A CA和下降的DREB 2A基因敲除植物。总的来说，这些结果表明DREB 2A在水和HS胁迫反应中起作用。少

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Functional analysis of an Arabidopsis transcription factor, DREB2A, involved in drought-responsive gene expression

DOI：
10.1105/tpc.105.035881
发表时间：
2006-05-01
期刊：
PLANT CELL
影响因子：
11.6
作者：
Sakuma, Y;Maruyama, K;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K

水分・温度ストレスに応答した転写制御ネットワーク

响应湿度和温度胁迫的转录调控网络

DOI：
发表时间：
2007
期刊：
蛋白質核酸酵素 52(6)
影响因子：
0
作者：
Tomoyuki Uchida;Hiroyuki Oura;Seiichi Ikeda;Fumihito Arai;Makoto Negoro;and Toshio Fukuda;佐久間洋・篠崎和子
通讯作者：
佐久間洋・篠崎和子

Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression

DOI：
10.1073/pnas.0605639103
发表时间：
2006-12-05
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Sakuma, Yoh;Maruyama, Kyonoshin;Yamaguchi-Shinozaki, Kazuko
通讯作者：
Yamaguchi-Shinozaki, Kazuko

Transcriptional regulatory networks in responses to water and temperature stresses in plants.

植物响应水和温度胁迫的转录调控网络。