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Molecular and biological analyses of a homeotic mutant and the regulation of floral organ formation in asparagus

芦笋同源突变体的分子和生物学分析以及花器官形成的调控

基本信息

批准号：
17380014
负责人：
MASUDA Kiyoshi
金额：
$ 10.53万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380014/
关键词：
asparagus PCR marker quantitative PCR supermale line MADS-box genes floral organs 雄性マーカー超雄性性判別ホメオティック変異 MADS-Box 遺伝子雌雄性 ABCモデル MADS-Box遺伝子 in situ ハイブダイゼーション

项目摘要

The gender of asparagus is known to be determined by a male active factor M. Some lines of asparagus cultivars carry a homozygous genotype for M. These lines are practically called supermale, since all of the progeny express male phenotype, when pollens from these lines have been used for pollinaton. Therefore, the establishment of supermale lines of asparagus has been tried, in order to produce seed populations only with male genotype. To isolate the homozygous genotype on M male asparagus plants with andromonoecous phenotype, which are able to seed by self-fertilization, have been used. However, lines with flowers capable of self-fertilization are extremely infrequent, and the establishment of supermale lines strongly depends on cultivars. Asparagus differentiate flowers initially as hermaphroditic morphology, and then mature into female and male flowers by degeneration of the inappropriate floral organ. The identity of floral organs is regulated under the expression of MADS-box genes. Flowers set on a line of 'Gold Schatz' (GS#2) form carpel-like organs at whorl 3, and the reduced expression of class-B genes was found to relate the homeotic change. In addition, floral organs at whorl 3 of 'GS#2' escape the possible degeneration and continue to develop until maturation. The progeny obtained by mating with wild-type asparagus showed only normal phenotype, suggesting that the mutation must be recessive. In future, the establishment of methodology to produce progeny showing the homeotic change may be required. On the other hand, for readily applicable research, we examined diagnostics of female and male asparagus. As a result, the utilization of DNA markers may be efficient for identifying gender of asparagus plants at the juvenile stage. Furthermore, supermale individuals were distinguishable from male individuals by quantitative PCR using the DNA markers, suggesting that supemale carries homozygous genotype on male-activating factor M.

已知芦笋的性别是由雄性活性因子M决定的。芦笋栽培品种的一些品系携带M的纯合基因型。这些品系实际上被称为超级雄性，因为当这些品系的花粉被用于授粉时，所有的后代都表现雄性表型。因此，人们尝试建立芦笋超雄性系，以产生仅具有雄性基因型的种子群体。为了分离具有雄性雌雄同体表型的M雄性芦笋植株上的纯合基因型，已经使用了能够通过自花受精而播种的植株。然而，具有能够自花受精的花的品系是极其罕见的，并且超级雄性品系的建立强烈地依赖于品种。芦笋的花最初是雌雄同体的，然后通过不适当的花器官退化而发育成雌花和雄花。MADS盒基因的表达调控花器官的同一性。在'Gold Schatz'（GS #2）系上的花在第3轮形成心皮状器官，并且发现B类基因的表达减少与同源异型变化有关。此外，‘GS #2’第3轮花器官避开了可能的退化，继续发育直至成熟。与野生型芦笋交配获得的后代仅表现正常表型，表明该突变为隐性突变。将来，可能需要建立产生显示同源异型变化的后代的方法。另一方面，对于易于应用的研究，我们检查了雌性和雄性芦笋的诊断。因此，利用DNA标记可以有效地鉴定芦笋幼苗期的性别。此外，利用DNA标记的定量PCR可以将超雄个体与雄性个体区分开来，表明超雄个体携带雄性激活因子M的纯合基因型。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

アスパラガスホメオティック突然変異'GS2'の交雑後代における変異回復について

关于芦笋同源异型突变“GS2”杂交后代的突变恢复

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Y.Asada;et al.;T. Ito;T. Fukuda;T. Fukuda;T. Fukuda;N.Murata et al.;安達由美子;Y. Adachi;安達由美子
通讯作者：
安達由美子

DNAマーカーを用いたアスパラガス雌雄判別法の簡便化と近縁種への応用

利用DNA标记简化芦笋性别判别方法及其在相关物种中的应用

DOI：
发表时间：
2006
期刊：
影响因子：
0
作者：
Y.Asada;et al.;T. Ito;T. Fukuda;T. Fukuda;T. Fukuda;N.Murata et al.;安達由美子;Y. Adachi;安達由美子;中山北斗;林有子;安達由美子;菅野明
通讯作者：
菅野明

Construction of a micro-library enriched with genomic replication origins of carrot somatic embryos by laser microdissection

通过激光显微切割构建富含胡萝卜体细胞胚胎基因组复制起点的微型文库

DOI：
发表时间：
2005
期刊：
Plant Physiology and Biochemistry 43
影响因子：
0
作者：
Y.Asada;et al.;T. Ito;T. Fukuda;T. Fukuda;T. Fukuda;N.Murata et al.
通讯作者：
N.Murata et al.

Identification of female, male and super-male asparagus plants by quantitative real-time PCR method

实时定量PCR法鉴定芦笋雌性、雄性和超雄性植株

DOI：
发表时间：
2006
期刊：
影响因子：
0
作者：
Y.Asada;et al.;T. Ito;T. Fukuda;T. Fukuda;T. Fukuda;N.Murata et al.;安達由美子;Y. Adachi;安達由美子;中山北斗;林有子;安達由美子;菅野明;K. Masuda;H. Nakayama;Y. Hayashi;Y. Adachi
通讯作者：
Y. Adachi

Molecular phylogeny of the genus Asparagus (Asparagaceae) inferred from plastid petB intron and petD-rpoA intergenic spacer sequences