Studies on micropropagation of female spinach plants and the stability of sex expression in the regenerants.
雌性菠菜植株的微繁殖及其再生体性别表达稳定性的研究。
基本信息
- 批准号:09660016
- 负责人:
- 金额:$ 0.7万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1998
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Somatic embryos obtained from the root callus of female or male plants of a oriental variety of spinach (Spinacia oleracea L.) were grown in vitro under long day length until flowering. Depending on the varieties and donor plants, 12.5 to 80.5% of regenerated plants flowered on a growth regulator-free medium. The plants derived from female explants were all female, while those from male donors included andromonoecy. The sex expression was pursued for plants regenerated from the regenerant-derived callus. The populations originating in female regenerants included gynomonoecy up to 63.7%, while 11.8 to 54.9% of those from male regenerants were andromonoecious. The application of gibberellic acid (GA3) elevated the ratio of andromonoecy but did not induce an absolute sex conversion, i.e., to the opposite sex.The effects of Ethephon, silver nitrate and aminoethoxyvinylglycine (AVG) on the embryogenic callus formation and embryogenesis were examined using tissue cultures of spinach root segments. The application of Ethephon increased the ratio of embryogenic callus formation. AgNO3 and AVG inhibited the embryogenic callus formation. Ethephon applied at 10muM during embryogenesis, however, completely inhibited the development of embryos. In contrast, the application of 10muM AgNO3 remarkably increased the number of embryos.
从东方菠菜(Spinacia oleracea L.)在体外长日照条件下生长直至开花。根据品种和供体植物的不同,12.5%至80.5%的再生植物在不含生长调节剂的培养基上开花。来自雌性外植体的植株均为雌性,而来自雄性供体的植株包括雄核单胞菌。对再生愈伤组织再生植株进行性别表达研究。雌株再生群体中雌雄同株占63.7%,雄株再生群体中雌雄同株占11.8%~ 54.9%。赤霉酸(GA 3)的应用提高了雄激素的比例,但没有诱导绝对的性别转换,即,以菠菜根段为材料,研究了乙烯利、硝酸银和氨基乙氧乙烯甘氨酸(AVG)对菠菜胚性愈伤组织形成和胚状体发生的影响。乙烯利处理提高了胚性愈伤组织的形成率。AgNO 3和AVG抑制胚性愈伤组织的形成。在胚胎发生期,10 μ M乙烯利处理完全抑制胚胎的发育。相反,10 μ M AgNO_3的应用显著增加了胚胎的数量。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MASUDA Kiyoshi其他文献
MASUDA Kiyoshi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MASUDA Kiyoshi', 18)}}的其他基金
Investigation of potential therapeutic strategies for esophageal squamous cell carcinoma focused on the functional modification of cancer stimulating RNA editing.
食管鳞状细胞癌潜在治疗策略的研究重点是癌症刺激 RNA 编辑的功能修饰。
- 批准号:
18K07910 - 财政年份:2018
- 资助金额:
$ 0.7万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Investigation of potential therapeutic targets for esophageal squamous cell carcinoma focused on the functional modification of cancer stimulating RNA binding protein.
食管鳞状细胞癌潜在治疗靶点的研究重点是癌症刺激 RNA 结合蛋白的功能修饰。
- 批准号:
15K08949 - 财政年份:2015
- 资助金额:
$ 0.7万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Identification of a novel tumor suppressive function of HIPK2 through RNA prosessing
通过 RNA 加工鉴定 HIPK2 的新型肿瘤抑制功能
- 批准号:
24590943 - 财政年份:2012
- 资助金额:
$ 0.7万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Elucidation of the molecular mechanism involved with the formation of hermaphrodite and unisexual flowers in asparagus.
阐明芦笋雌雄同体和单性花形成的分子机制。
- 批准号:
20380014 - 财政年份:2008
- 资助金额:
$ 0.7万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular and biological analyses of a homeotic mutant and the regulation of floral organ formation in asparagus
芦笋同源突变体的分子和生物学分析以及花器官形成的调控
- 批准号:
17380014 - 财政年份:2005
- 资助金额:
$ 0.7万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular and morphological analyzes of floral organs in an asparagus homeotic mutant
芦笋同源异型突变体花器官的分子和形态学分析
- 批准号:
15380018 - 财政年份:2003
- 资助金额:
$ 0.7万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies on the occurrence of monoecious variants during vegetative propagation of spinach and asparagus plants by tissue culture techniques
利用组织培养技术研究菠菜和芦笋植物无性繁殖过程中雌雄同株变异的发生
- 批准号:
12660018 - 财政年份:2000
- 资助金额:
$ 0.7万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Isolation and characterization of the nuclear matrix in higher plant cells
高等植物细胞核基质的分离和表征
- 批准号:
05640738 - 财政年份:1993
- 资助金额:
$ 0.7万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似海外基金
Development of a three-dimensional hard tissue culture system using inorganic polyphosphate and soft-tissue cells.
使用无机多磷酸盐和软组织细胞开发三维硬组织培养系统。
- 批准号:
23K09222 - 财政年份:2023
- 资助金额:
$ 0.7万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Physiologically relevant cardiac tissue culture model for drug testing and disease modeling
用于药物测试和疾病建模的生理相关心脏组织培养模型
- 批准号:
10654152 - 财政年份:2023
- 资助金额:
$ 0.7万 - 项目类别:
Creation of 3D tissue culture system integrated with blood vessels and autonomic nerves
打造血管与植物神经融合的3D组织培养系统
- 批准号:
23H01827 - 财政年份:2023
- 资助金额:
$ 0.7万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Mechanistic analysis of Toxoplasma gondii sexual development in tissue culture and mouse models
组织培养和小鼠模型中弓形虫性发育的机制分析
- 批准号:
10533513 - 财政年份:2022
- 资助金额:
$ 0.7万 - 项目类别:
Understanding effects of formulations on ingredient interaction with tissue culture models of the oral epithelium
了解配方对成分与口腔上皮组织培养模型相互作用的影响
- 批准号:
2747248 - 财政年份:2022
- 资助金额:
$ 0.7万 - 项目类别:
Studentship
Mechanistic analysis of Toxoplasma gondii sexual development in tissue culture and mouse models
组织培养和小鼠模型中弓形虫性发育的机制分析
- 批准号:
10738256 - 财政年份:2022
- 资助金额:
$ 0.7万 - 项目类别:
Physiologic stress in advanced tissue culture models of cardiomyopathy
心肌病高级组织培养模型中的生理应激
- 批准号:
10592151 - 财政年份:2022
- 资助金额:
$ 0.7万 - 项目类别:
WORKSHOP: Training the Next Generation of Workers and Outreach in Plant Tissue Culture for Crop Improvement Using Genome Editing
研讨会:培训下一代工人并推广植物组织培养,利用基因组编辑改良作物
- 批准号:
2220889 - 财政年份:2022
- 资助金额:
$ 0.7万 - 项目类别:
Standard Grant