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Construction of linkage maps in Radish and QTL analysis of root traits

萝卜连锁图谱构建及根性状QTL分析

基本信息

批准号：
17380006
负责人：
HIRAI Masashi
金额：
$ 10.93万
依托单位：
Kyoto Prefectural University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380006/
关键词：
Raphanus sativus Simple sequence repeat linkage map Root shape flowering genetic diversity clubroot resistance phylogeny 系統関係野生集団根こぶ病抵抗性 QTL 抽台マイクロサテライト根部形質形態形成

项目摘要

A linkage map was constructed by the group of Kyoto Prefectural University and Meijo University using a segregating population derived from a crossing of a Chinese radish having spherical root and a Japanese radish having cylindrical root. In this map, we identified QTLs for root shape and root anthocyanin pigmentation. We also mapped clubroot resistance loci by a QTL analysis. The results indicate that the resistance is controlled by a major gene or closely linked major genes. Vernalization-induced flowering of radish was studied another segregating population derived from a crossing of rat-tailed radish from South-east Asia and a Japanese radish. The former parent does not required low temperature for flowering unlike Japanese radishes. The flowering is controlled by a single major gene. Linkage markers for the flowering gene were obtained by a bulked segregant analysis. The linkage makers showed base homologies with genomic sequences in lower arm of chromosome 1 of Arabidopsis.Another linkage map was constructed by the group of Tsukuba University based on a crossing of Shogoin radish and a Taiwanese radish. QTLs for root shape are obtained. Anumber of genetic markers for radish were developed by this group.Phylogenic relationship and genetic diversity were studied using radish germplasm collected United States and Eurasian continent. They are grouped by the original area of the lines. Any bottle neck was observed as far as examined.Genetic relationship and the diversity of Shogoin radishes and other Japanese radish were studied. Shogoin radishes showed markedly low genetic diversity calculated by allele frequency of SSR.

京都府立大学和名城大学的研究小组利用具有球形根的中国萝卜和具有圆柱形根的日本萝卜杂交所得的分离群体构建了连锁图谱。在此图中，我们鉴定了根形状和根花青素色素沉着的 QTL。我们还通过 QTL 分析绘制了根肿病抗性位点。结果表明，抗性是由一个主基因或紧密连锁的主基因控制的。研究了由来自东南亚的鼠尾萝卜和日本萝卜杂交产生的另一个分离种群，研究了春化诱导的萝卜开花。与日本萝卜不同，前亲本开花不需要低温。开花由一个主基因控制。通过批量分离分析获得开花基因的连锁标记。连锁标记与拟南芥1号染色体下臂的基因组序列显示出碱基同源性。筑波大学研究小组基于圣护院萝卜和台湾萝卜的杂交构建了另一个连锁图谱。获得根形状的QTL。该课题组开发了多个萝卜遗传标记。利用美国和欧亚大陆采集的萝卜种质资源，对系统发育关系和遗传多样性进行了研究。它们按线条的原始面积分组。对圣护院萝卜和其他日本萝卜的遗传关系和多样性进行了研究。根据 SSR 等位基因频率计算，圣护院萝卜的遗传多样性明显较低。