Physiological and molecular biological analysis of some factors related to fit softening and storability

贴合软化及耐贮性相关因素的生理和分子生物学分析

• 批准号: 17380023
• 负责人: ITAMURA Hiroyuki
• 金额: $ 10.72万
• 依托单位: Shimane University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380023/
• 关键词: Fruit softening; on tree ripening; Expressed sequence tag; Magnetic resonance imagine; Plant hormone; EST解析; MRI断層撮影; 樹状成熟

To obtain the basic information for improving fruit storability, we investigated fruit development and ripening on a tree, and softening after harvest by physiological, histochemical and molecular biological approach.A total of 15680 expressed sequence tags were generated from randomly selected clones of three different cDNA libraries in 'Saijo' persimmon; fruit at an early stage of development, ripening fruit, and rapidly softened fruit on a tree. We successfully obtained a set of genes that are expressed during fruit development and ripening in persimmon. We have obtained a full length and a partial cDNA encoding arabinofuranosidase and xylosidase/arabinofuranosidase, respectively from persimmon cDNA library. The higher expression of both genes were in accordance with rapid softening. This result indicates that both genes may play an important role in softening after artificial de-astringency.As the treatment of 1-methylcyclopropene, ethylene action inhibitor; did not affect fruit coloration in 'Saijo' persimmon fruit on a tree, spontaneously and transiently produced internal ethylene of the fruit on a tree is unrelated to coloration on a tree. On the other hand, fruit softening like a jerry in late autumn on a tree is corresponding to increase of internal ethylene. In grape berries and blueberry fruit, the changes in the inner contents were investigated during the growth and ripening period. It is cleared that the growth rate of grape berries is quite different from each part of tissue and the cell wall contents of blueberry fruit are different among the picking times.Clear Magnetic Resonance Imaging (MRI) images were obtained in softening 'Saijo' Japanese persimmon fruit witch was treated with ethylene. Nondestructive method, such as MRI imaging, enabled internal observation of very soft persimmon fruit. Infiltration of fruit tissue seemed to begin around vascular bundles of fruit. Loosening of tissue was observed by photo-microscopy in softening fruit.

为了获得提高果实耐贮性的基础信息，本研究采用生理学、组织化学和分子生物学方法，对“西条”柿果实的发育、成熟和采后软化进行了研究。果实发育早期的果实、成熟的果实和树上迅速变软的果实。我们成功地获得了一组在柿果实发育和成熟过程中表达的基因。我们从柿cDNA文库中获得了阿拉伯呋喃糖苷酶和木糖苷酶/阿拉伯呋喃糖苷酶的全长和部分cDNA。这两个基因的高表达与快速软化相一致。由于乙烯作用抑制剂1-甲基环丙烯处理对“西条”柿果实的着色没有影响，因此果实内部自发产生的乙烯与果实着色无关。另一方面，晚秋果实软化如杰里树对应于内部乙烯的增加。以葡萄和蓝莓果实为材料，研究了果实生长和成熟过程中果实内可溶性糖含量的变化。结果表明，不同采收期蓝莓果实各组织的生长速度不同，细胞壁含量也不同，乙烯处理后的“西条”日本柿果实软化过程中，获得了清晰的磁共振成像（MRI）图像。非破坏性的方法，如磁共振成像，使内部观察非常软的柿子果实。果实组织的渗透似乎是从果实的维管束周围开始的。光镜下观察到果实软化过程中组织疏松。

项目成果

Enzymatic activities and gene expression of l-aminocyclopropane-l-carboxylic acid (ACC) synthase and ACC oxidase in Jananese persimmon fruit

日本柿果实中l-氨基环丙烷-l-羧酸（ACC）合酶和ACC氧化酶的酶活性和基因表达

• 发表时间: 2005
• 期刊: Postharvest Biol. Technol 37
• 作者: Zheng;Q-L.;A.;Nakatsuka;S.;Taira;H.;Itamura
• 通讯作者: Itamura

Effects of wound treatment to fruit on a tree on ethylene induction and the role of leaves

树上果实伤口处理对乙烯诱导的影响和叶子的作用

• 发表时间: 2007
• 作者: Sun;N.;Y.;Hanaoka;S.;Naritoku;A.;Nakatsuka;H.;Itamura
• 通讯作者: Itamura

日本食品保蔵科学会 学会賞総説「カキ果実の成熟および脱渋後の軟化に関する研究」 32(2)

日本食品保鲜学会学术奖审查《柿子的成熟及去涩后软化的研究》32(2)

• 发表时间: 2006
• 作者: Itamura;H;板村裕之
• 通讯作者: 板村裕之

1-MCPがナシ果実貯蔵中の糖代謝に及ぼす影響.

1-MCP对梨果实贮藏过程中糖代谢的影响

• 发表时间: 2008
• 作者: Franco-Mora;O.;Franco-Mora O.;Franco-Mora O.;板村裕之・花岡陽介・中本哲義・成徳 聡・孫 寧静.;板井章浩・棚橋拓郎・田辺賢二.
• 通讯作者: 板井章浩・棚橋拓郎・田辺賢二.

カキ'西条'果実の熟柿化過程における裂果.

柿子“西条”果实在成熟过程中破裂。

• 发表时间: 2007
• 作者: Nakatsuka;A.;T.;Nakagawa;K.;Yann;A.;Ttai;N.;Kobayashi;H.;Itamura;板井章浩・棚橋拓郎・田辺賢二.;中務 明・中川 強・矢野健太郎・板井章浩・小林伸雄・板村裕之.;花岡陽介・孫 寧静・中務 明・板村裕之.;赤浦和之.
• 通讯作者: 赤浦和之.