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Integrated study on molecular evolution and biological functions of fish lectins

鱼类凝集素分子进化与生物学功能的综合研究

基本信息

批准号：
17380122
负责人：
MURAMOTO Koji
金额：
$ 10.22万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17380122/
关键词：
lectin sugar binding protein sugar chain marine bioactive protein animal lectin family biodefence fish egg rhamnose ガレクチングルゲア胞子インターロイキン RTG-2細胞サケ科魚類卵レクチンマクロファージ RTM-5細胞

项目摘要

The evolutionary process of the conger eel galectins, congerin I and II, was investigated by preparing a probable ancestral form of congerins, Con-ans, on the basis of phylogenetic tree. The comparison among their thermostabilities and sugar specificities showed that congerins have evolved via accelerated evolution under significant selective pressure to improve their functions. The multiple C-type lectins, BRA-1, -2 and -3, from Megabalanus rosa, had characteristic and distinct sugar binding specificities. The lectins also had modulating effects on the crystallization of calcium carbonate, indicating their participation in biomineralization. Multiple L-rhamnose-binding lectins (RBLs) were isolated and characterized from the eggs of various fish species; i.e. steelhead trout, chum salmon, Spanish mackerel, and ayu. RBLs were composed of homologous subunits with 2-3 tandemly repeated domains of about 95 amino acid residues. Each domain could be characterized by highly conserved 8 half-C … More ys residues at homologous positions as well as the conserved peptide motifs. RBLs specifically recognized and bound to globotriaosylceramide (Gb3), and agglutinated Gram-negative and Gram-positive bacteria by recognizing the structures of lipopolysaccharides and lipoteichoic acid on their surfaces, respectively. They also interacted with the spores of a microsporidian parasite. RBLs from chum salmon eggs, CSL1, 2 and 3, induced proinflammatory cytokines by recognizing Gb3 on the surface of the peritoneal macrophage cell line (RTM5 cells) from rainbow trout. In addition, CSLs had an opsonic effect on RTM5 cells by binding to Gb3. CSLs induced the production of reactive oxygen species in dose-dependent manner in RTM5 cells. This activity was not inhibited by L-rhamnose, indicating that the sugar binding site of CSLs was not involved in the activity. Moreover, some RBLs induced apoptosis of human colon cancer cells by recognizing Gb3 on the cells. These properties of RBLs indicate that they may function as biodefence molecules. Less

通过制备一种可能的海鳗半乳糖凝集素的祖先形式Conans，在系统发育树的基础上，研究了海鳗半乳糖凝集素Congerin I和II的进化过程。通过对它们的热稳定性和糖特异性的比较表明，在显著的选择压力下，congerins通过加速进化来改善其功能。结果表明，从玫瑰Megabalanus rosa中分离到的多种C型凝集素BRA-1、BRA-2和BRA-3具有独特的糖结合特性。凝集素对碳酸钙的结晶也有调节作用，表明它们参与了生物矿化。多种L-鼠李糖结合凝集素（RBL）的分离和特点，从各种鱼类的卵，即虹鳟鱼，鲑鱼，西班牙鲭鱼，香鱼。RBL由2-3个串联重复的95个氨基酸残基的同源亚基组成。每个结构域都具有高度保守的8个半C ...更多信息 ys残基以及保守的肽基序。RBL特异性地识别并结合至神经酰胺三己糖苷（Gb 3），并通过分别识别其表面上的脂多糖和脂磷壁酸的结构而凝集革兰氏阴性菌和革兰氏阳性菌。它们还与微孢子虫寄生虫的孢子相互作用。来自鲑鱼卵的RBL，CSL 1，2和3，通过识别虹鳟鱼腹腔巨噬细胞系（RTM 5细胞）表面上的Gb 3诱导促炎细胞因子。此外，CSL通过与Gb 3结合对RTM 5细胞具有调理作用。CSL诱导RTM 5细胞产生活性氧，并呈剂量依赖性。该活性不受L-鼠李糖的抑制，表明CSL的糖结合位点不参与该活性。此外，一些RBL通过识别细胞上的Gb 3诱导人结肠癌细胞凋亡。RBL的这些性质表明它们可能作为生物防御分子发挥作用。少