Reasonable recellularization of the decellularized pulmonary valve in vivo

背景和目的：目前已有多种技术用于异体/异种脱细胞心脏瓣膜和组织工程化生物降解支架的再细胞化，但脱细胞基质支架适合受体细胞再细胞化的条件和移植物再生机制尚不清楚。本研究的目的是从基质结构的角度寻找最佳的心脏瓣膜脱细胞技术，并利用仔猪体内模型评价受体细胞的再细胞作用。Adequate脱细胞技术=新开发的氢氧化铵-Triton法，即使是0.1%的氢氧化铵浓度，心脏瓣膜组织也完全脱细胞。虽然用氢氧化铵-Triton法可使主要基质结构弹性蛋白和胶原纤维保持较好的状态，但糖胺聚糖纤维大部分已基本消失。关于Di…脱细胞肺动脉瓣体内再细胞化：将猪去细胞单瓣移植到同种异体仔猪模型的颈内静脉，4周后取材。免疫组织化学显示α-SMA阳性细胞迁移到移植物组织的表层和内层，vWF阳性细胞覆盖于移植物表面。结论：从基质组织保存的角度出发，0.1%氢氧化铵-Triton法是一种合适的脱细胞技术。这些关于体内植入的脱细胞肺动脉瓣再细胞化的数据表明，受体细胞在脱细胞基质支架上迁移到移植物中。关于移植物的合理再细胞化，需要进一步的检查以排除炎细胞的渗透，并具有良好的血流动力学和耐久性，以便更好地在未来的临床应用中应用。较少

Background and Objectives : Although various technologies to recellularize both allogenic/xenogenic decellularrized heart valves and tissue-engineered biodegradable scaffolds have been developed, adequate condition of the decellularized matrix scaffold for recellularization by recipient cells and mechanism of regeneration of these grafts remain to be elucidated. The purpose of this study was to find optimal decellularizing techniques for the heart valve from the point of the matrix structure and to evaluate the recellularization by recipient cells using piglet model in vivo.Adequate decellularization techniques= Newly developed ammonium hydroxide-triton method, even the 0.1% concentration of ammonium hydroxide, decellularized the heart valve tissue completely. Although the elastin and collagen fiber, which is the main matrix structure, was maintained with good condition by the ammonium hydroxide-triton method, most of glycosaminoglycan fiber has almost disappeared. Concerning on the di … More sappearance of glycosaminoglycan, further examination to preserve the glycosaminoglycan fiber will be required for better decellularization.Recellularization of the decellularized pulmonary valve in vivo : Porcine decellularized monocusp valve graft was implanted to the internal jugular vein of allogenic piglet model, and was harvested four weeks after implantation. Immunohistological examination showed that α-SMA positive cells migrated into the surface and inner layer of the graft tissue, and vWF positive cells covered the surface of it. Matrix structure of the graft seemed to be preserved, however, massive inflammatory cells infiltrated into the graft tissue.Discussion : We developed 0.1% ammonium hydroxide-triton method as an adequate decellularization technique from the point of matrix tissue preservation. These data on recellularization of the decellularized pulmonary valve implanted in vivo imply that recipient cells migrate into the graft with decellularized matrix scaffold. Concerning on reasonable recellularization of the graft, further examination to exclude the inflammatory cells infiltration and to function with good hemodynamics and durability will be required for better decellularization technology in future clinical use. Less