Basicresearch for visualization of functional neuronal tract by gene transfer into CNS neurons

通过基因转移到中枢神经系统神经元实现功能神经束可视化的基础研究

• 批准号: 17390396
• 负责人: KATO Ichiro
• 金额: $ 10.6万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390396/
• 关键词: rhodopsin; promoter; EGFP; retina; pineal body; neuron; photoreceptor; tracer; Rhodosin; promoter; 蛍光蛋白質; 神経回路; 神経細胞; アデノウィルス; 遺伝子導入; 逆行性軸索輸送; 投射ニューロン; 機能マッピング

1. The aim of this study is to visualize neuronal tract by expression of enhanced green fluorescent protein (EGFP) in neurons of the central nervous system.2. We analyzed transgenic mice that express EGFP under the control of the mouse rhodopsin gene promoter.3. In Western blot analyses of transgenic retinal homogenates, EGFP expression in transgenic retinas was initially detected at P12.4. At P14, fluorescence microscopy showed a weak expression of EGFP in the transgenic retina. In the adult transgenic mice, the strongest EGFP expression was observed in the outer nuclear layer, and to a lesser extent in the outer plexiform layer as well as in the inner and outer segments.5. EGFP expression was also observed in the pineal region, especially in the pineal stalk.6. We produced transgenic mice overexpressing EGFP under the control of GFAP promoter and we detected EGFP expression in their brains.7. We produced transgenic mice overexpressing EGFP under the control of Thy 1.2 promoter.8. Several transgenic mice expressing EGFP in neurons in central nervous system were established in this study. These transgenic mice will be useful to locate neuronal tract in vivo, by precisely determining neuronal cells that are fluorescent green.

1.本研究的目的是通过增强型绿色荧光蛋白（EGFP）在中枢神经系统神经元中的表达来显示神经束.我们分析了在小鼠视紫红质基因启动子控制下表达EGFP的转基因小鼠.在转基因视网膜匀浆的Western印迹分析中，在P12.4时最初检测到转基因视网膜中的EGFP表达。在P14，荧光显微镜显示在转基因视网膜中有弱的EGFP表达。在成年转基因小鼠中，最强的EGFP表达出现在外核层，在外网层以及内外节中的表达程度较低. EGFP在松果体区也有表达，尤其是在松果体柄.制备了GFAP启动子调控下的EGFP过表达转基因小鼠，并检测了其脑组织中EGFP的表达.我们在Thy 1.2启动子的控制下产生了过表达EGFP的转基因小鼠。本研究建立了几只在中枢神经系统神经元中表达EGFP的转基因小鼠。这些转基因小鼠将有助于通过精确确定荧光绿色的神经元细胞来定位体内神经束。

项目成果

Transparent endoscopic sheath and rigid-rod endoscope used in endoscopic third ventriculostomy for hydrocephalus in the presence of deformed ventricular anatomy

透明内镜鞘和硬棒内镜用于内镜下第三脑室造口术治疗脑室解剖变形的脑积水

• 发表时间: 2006
• 期刊: J. Neurosurg 104
• 作者: Havashi;N;Hamada;H;Umemura;K;Kurosaki;K;Kurimoto;M;Endo;S
• 通讯作者: S

Neuronal responses to a delayed-reward go/nogo task in the monkey posterior insular cortex

猴子后岛叶皮层对延迟奖励 go/nogo 任务的神经元反应

• 发表时间: 2006
• 期刊: Neuroscience 143
• 作者: Asahi;T;Uwano;T;Eifuku;S;Tamura;R;Endo;S;Ono;T;Nishijo;H
• 通讯作者: H

Warning headache of subarachnoid hemorrhage and infarction due to vertebrobasilar artery dissection

椎基底动脉夹层蛛网膜下腔出血和梗死的警告性头痛

• 发表时间: 2006
• 期刊: Clin. J. Pain 22
• 作者: Shibata;T;Kubo;M;Kuwayama;N;Hirashima;Y;Endo;S
• 通讯作者: S

Stereotactic Voa-Vop complex thalamotomy for writer's cramp

立体定向 Voa-Vop 复合丘脑切除术治疗作家痉挛

• 发表时间: 2005
• 期刊: Eur. Neurol 53
• 作者: Shibata;T;Hirashima;Y;Ikeda;H;Asahi;T;Hayashi;N;Endo;S
• 通讯作者: S

家族性早期発症型認知症FENIBモデルマウスにおいて変異型ニューロセルピン(G392E)は小胞体およびリソソーム内に蓄積する.

突变型神经丝氨酸蛋白酶抑制剂 (G392E) 在 FENIB 家族早发性痴呆模型小鼠的内质网和溶酶体中积累。

• 发表时间: 2007
• 作者: Hirashima;Y;Kurimoto;M;Hayashi;N;Umemura;K;Hori;E;Origasa;H;Endo;S;Ichsan AM.;Hirashima Y.;Hirashima Y.;Nagai S.;Hamada H.;Hori E.;Kuwayama N.;Hayakawa Y.;Kanekiyo K.;Hirashima Y.;Hayashi N.;Kurosaki K.;Hirashima Y.;Shibata T.;Tada M.;鈴木ひかり;柴田 孝;柴田 孝;加藤一郎
• 通讯作者: 加藤一郎