Expansion of application fields and conversion of examination methods far personal identification using DNA polymorphisms in the field of Forensic Odontology

法医牙科学领域利用DNA多态性进行个人识别的应用领域的扩展和检查方法的转换

• 批准号: 17390567
• 负责人: MINAGUCHI Kiyoshi
• 金额: $ 6.52万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390567/
• 关键词: Mitochondrial DNA polymorphisms; Y chromosomal polymorphisms; Pylogenetic differentiation; Xchromosomal polymorphisms; Estimation of geographic orisin; Malay population; Autosonao polymorphisms; Material examination; mtDNA haplogroup; Y-STR多型; Y

The purpose of this study is conversion of examination methods to develop reliability of DNA analysis from highly degraded materials, and the expansion of targets on chromosomal DNA to use DNA analysis not only for personal identification but also for sex determination, estimation of geographic origin, and human-animal discrimination. The results obtained were as follows, 1. We found 13 new mitochondrial haplogroup lineages from 100 Japanese individuals and established them by complete sequencing. We further showed that general Malay population was composed of East Asian and South Asian mitochondrial haplogroup lineages, and established several new lineages characteristic to the Malay population under updated information. These data are useful for estimating geographic origin of individuals in East Asia. 2. We presented database on relationship between 16 Y-STRs contained in AmpFISTR Yfiler kit and Y binary haplogroups. This data is applicable for selecting Japanese individuals from Asian population. We found new clades in O haplogroup lineage of Y-chromosome polymorphism in a Malay population. This information is useful for estimating geographic origin of individuals in Asia. 3. We developed X-STR multiplex PCR systems and analyzed frequencies of 16 STR loci in the Japanese population. 4. We examined 15 autosomal STR loci in a Malay population, and showed characteristic of the Malay population in Asia. 5. We compared PCR amplification efficiency from degraded DNA using 2 kinds of STRs, and showed that amplification efficiency is highly influenced by PCR amplification sizes, and also showed that SNP analysis was more suitable for examination of degraded DNA in a forensic case.Recent progress on research related to the present study was very rapid and we could not but arrange part of our original purposes. However, we believe that we could obtain useful results for DNA analysis in practice.

本研究的目的是转换检测方法，从高度降解的材料中开发DNA分析的可靠性，并扩大染色体DNA的目标，使DNA分析不仅用于个人识别，还用于性别确定，地理来源估计和人-动物区分。得到的结果如下：1。我们从100个日本个体中发现了13个新的线粒体单倍群谱系，并通过完全测序建立了它们。我们进一步发现马来人群是由东亚和南亚线粒体单倍群谱系组成的，并在最新信息下建立了几个具有马来人群特征的新谱系。这些数据对于估计东亚地区个体的地理来源是有用的。我们建立了AmpFISTR Yfiler kit中包含的16个Y- str与Y二元单倍群的关系数据库。这一数据适用于从亚洲人口中选择日本人。我们在马来人群的y染色体多态性O单倍群谱系中发现了新的分支。这一信息对于估计亚洲个体的地理来源是有用的。我们开发了X-STR多重PCR系统，并分析了日本人群中16个STR位点的频率。4. 我们在马来人群体中检测了15个常染色体STR位点，并显示了亚洲马来人群体的特征。我们比较了两种str对降解DNA的PCR扩增效率，发现扩增效率受PCR扩增大小的影响较大，同时也表明SNP分析更适合法医案例中降解DNA的检验。最近与本研究相关的研究进展非常迅速，我们不得不安排部分原始目的。然而，我们相信我们可以在实践中获得有用的DNA分析结果。

项目成果

Population data on 15 STR loci using AmpF/STR Identifiier kit in a Malay population living in and around Kuala Lumpur, Malaysia

使用 AmpF/STR Identifiier 试剂盒对居住在马来西亚吉隆坡及其周边地区的马来人群中的 15 个 STR 位点进行人口数据

• 发表时间: 2008
• 期刊: Legal Med 10
• 作者: Maruyama;S;Minaguchi;K;Takezaki;N;Nambiar;P
• 通讯作者: P

顎顔面領域の情報からの個人識別

根据颌面部信息进行个人识别

• 发表时间: 2007
• 期刊: 日法医誌 61
• 作者: Nonaka I;Minaguchi K;Takezaki N;水口 清
• 通讯作者: 水口 清

全ゲノム配列による比較から見たマレーシア・クアラルンプール周辺に在住するマレー人のmtDNAの系統評価

基于全基因组序列比较，对居住在马来西亚吉隆坡附近的马来人 mtDNA 进行系统发育评估

• 发表时间: 2007
• 作者: 丸山 澄;野平 千鶴;佐々木 継泰;石川 昴;水口 清;Nambiar P
• 通讯作者: Nambiar P

日本入におけるmtDNA多型系統の枝葉

日本进口线粒体DNA多态性分支

• 发表时间: 2005
• 期刊: DNA多型 Vol.13
• 作者: 丸山 澄;水口 清
• 通讯作者: 水口 清

Identification of unknown body using DNA analysis and dental characteristics in chest X-ray photograph

利用胸部 X 光照片中的 DNA 分析和牙齿特征识别未知尸体

• 发表时间: 2005
• 期刊: Bull Tokyo Dent Coll 46(4)
• 作者: Minaguchi K;Maruyama S;Kasahara;I;Nohira C;Hanaoka Y;Tsai T;Kiriyama T;Takahashi N
• 通讯作者: Takahashi N