Studies on the Relationship Between Salivary Proline-Rich Protein Polymorphisms and the DNA Loci.

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1. Four kinds of slow-migrating Gl proteins (Gl4J, 5, 6, 7) were purified and amino acid compositions were analyzed. All the proteins were proved to be the same kinds of Gl protein.2. Under collaborative research with Dr. Azen, new variant of Gl protein (Gl8) was found. A previous study of this variant protein misidentified it as Pa2 and was a products of PRH1 locus. The other PRB3 allele in this individual was a null.3. Slow-migrating Gl variants were examined in Chinese, Malays, Indians and Filipinos. These variant alleles were restricted to Mongoloids, and each allele was biased within Asian populations. Gl4 observed in whites was different from our slow-migrating Gl. In addition, new allele (Gl10) was found in Filipinos, which leads to the existence of 12 alleles in PRB3 locus.4. Assuming that basic PRPs were produced from 4 loci, PmF, PmS, Ps, Gl, Pmo1, Pmo2, P3 proteins were classified through family studies into 4 groups, that is, PmF/PmS/Pmo1, Gl, Ps/Pmo2 and P3.We would like to continue to establish the relationships between salivary basic PRPs and DNA loci. PRP Loci are highly polymorphic and will be useful for personal identification in forensic medicine and anthropological studies.