Studies on the Relationship Between Salivary Proline-Rich Protein Polymorphisms and the DNA Loci.

唾液富含脯氨酸蛋白多态性与DNA位点关系的研究。

• 批准号: 01571029
• 负责人: MINAGUCHI Kiyoshi
• 金额: $ 0.77万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01571029/
• 关键词: Proline-rich Protein; Salivary Protein; Genetic Polymorophisms; PRB Loci; Basic PRP; 遺伝子多型

1. Four kinds of slow-migrating Gl proteins (Gl4J, 5, 6, 7) were purified and amino acid compositions were analyzed. All the proteins were proved to be the same kinds of Gl protein.2. Under collaborative research with Dr. Azen, new variant of Gl protein (Gl8) was found. A previous study of this variant protein misidentified it as Pa2 and was a products of PRH1 locus. The other PRB3 allele in this individual was a null.3. Slow-migrating Gl variants were examined in Chinese, Malays, Indians and Filipinos. These variant alleles were restricted to Mongoloids, and each allele was biased within Asian populations. Gl4 observed in whites was different from our slow-migrating Gl. In addition, new allele (Gl10) was found in Filipinos, which leads to the existence of 12 alleles in PRB3 locus.4. Assuming that basic PRPs were produced from 4 loci, PmF, PmS, Ps, Gl, Pmo1, Pmo2, P3 proteins were classified through family studies into 4 groups, that is, PmF/PmS/Pmo1, Gl, Ps/Pmo2 and P3.We would like to continue to establish the relationships between salivary basic PRPs and DNA loci. PRP Loci are highly polymorphic and will be useful for personal identification in forensic medicine and anthropological studies.

1.对4种慢迁移G1蛋白(Gl4J、5、6、7)进行了纯化，并对其氨基酸组成进行了分析。结果：1.所有蛋白均为相同类型的Gl蛋白。在与艾岑博士的合作研究中，发现了新的Gl蛋白变体(GL8)。先前对该变异蛋白的研究错误地将其识别为Pa2，是PRH1基因座的产物。该个体的另一个PRB3等位基因为空。在中国人、马来人、印度人和菲律宾人中检测了迁移缓慢的Gl变异。这些变异等位基因仅限于蒙古族，而且每个等位基因在亚洲群体中都存在偏见。在白人中观察到的GL4不同于我们缓慢迁移的Gl。此外，在菲律宾人中发现了新的等位基因(GL10)，导致在PRB3基因座上存在12个等位基因。假定PmF、Pms、Ps、G1、Pmo1、Pmo2、P3蛋白由4个基因座产生，家族研究将PmF/Pms/Pmo1、G1、Ps/Pmo2和P3蛋白分为4组，即PmF/Pms/Pmo1、G1、Ps/Pmo2和P3。PRP基因座具有高度的多态性，在法医学和人类学研究中将用于个人身份识别。

项目成果

水口 清: "唾液プロリンリッチプロテインの個人的変異" 歯科学報. 90. 1183-1196 (1990)

Kiyoshi Mizuguchi：“唾液富含脯氨酸的蛋白质的个体变异”《牙科杂志》90。1183-1196（1990）。

Minaguchi K.: "Salivary proline-rich protein polymorphisms." Shikwa Gakuho. 90. 1183-1196 (1990)

Minaguchi K.：“唾液富含脯氨酸的蛋白质多态性。”

Azen,E.A.et.al.: "Alleles at the PRB3 locus coding for a disulfideーbonded human salivary prolineーrich glycoprotein(Gl8) and a null in an Ashkenazi Jew." Amer,J,Hum,Genet.47. 686-697 (1990)

Azen,E.A.等人：“PRB3 基因座上的等位基因编码二硫键连接的人类唾液富含脯氨酸的糖蛋白 (G18)，而在德系犹太人中则为空。” 686-。 697 (1990)

Azen, E. A., Minaguchi, K., Latreille, P. and Kim, H. S.: "Allese at the PRB3 locus coding for a disulfide-bonded human salivary proline-rich glycoprotein (Gl8) and a null in an Ashkenazi Jew." Amer. J. Hum. Genet. 47. 686-697 (1990)

Azen, E. A.、Minaguchi, K.、Latreille, P. 和 Kim, H. S.：“PRB3 基因座上的 Allese 编码二硫键连接的人唾液富含脯氨酸的糖蛋白 (Gl8)，而德系犹太人中则为空。”

Azen,E.A.et al.: "Alleles at the PRB3 locus coding for a disulfide bonded human salivay proline-rich glycoprptein(G1 8)and a null in an Ashkenazi Jew." Am.J.Hum.Genet.

Azen,E.A.等人：“PRB3 基因座上的等位基因编码二硫键结合的人唾液富含脯氨酸的糖蛋白 (G1 8)，而德系犹太人中的等位基因无效。”