Molecular Evolutional, Geographical, Population Genetical analyses of Wild SilkMoth including Bombyx mandarina distribute in East Asian region

东亚地区野生蚕蛾（包括家蚕）的分子进化、地理、种群遗传分析

• 批准号: 17405023
• 负责人: NAKAJIMA Yumiko
• 金额: $ 8.82万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17405023/
• 关键词: Bombyx mandarina; Wild Silk moth; mariner-like-elements (MLEs); Mitochondria Cox1 gene; ALP gene (Alp-m, Alp-s); Intergenic region; Vitellogenin gene; rDNA region; Cecropia-ITR-MLE; アルカリフォスファターゼ(ALP); (Alp-m,Alp-s); ミトコンドリア(mt)cox遺伝子; クワコ(Bomb

We have analyzed the molecular level of polymorphism in Bombyx mandarina, some other wild silk moth species distribute in wide range of East Asian region by comparing some genes or DNA marker regions among them. B. mori molybdenum cofactor sulfurase gene (og) whose mutants cause translucent skin because of lack or deficiency of accumulation of uric acid under the skin in B. mori. Og genes from Japanese mandarina have wide variety and different from the group of B. mori. We conducted an analysis of mitochondrial coxl gene of B. mori and found low level of sequence diversity relative to that of the og gene. Alkaline phosphatase (Alp) genes are tandemly duplicated in B. mori. The Alp genes (Alp-m, Alp-s) share about 60% identity in the nucleotide sequences, but structure of the intervening region is highly polymorphic with large in/del from strain to strain. In this study we found that the Alp genes were shown to be duplicated in B. mandarina, similarly as in B. mori, in all four gemones. The structure of the intervening region in B. mandarina suggested that the most recent common ancestor of the current silk worm strains and wild B. mandarina existed in China. From the analysis of Cecropia-ITR-MLEs, most of the MLE sequences from B. mandarina in the main island of Japan composed one unique group (Japanese type); on the other hand, the sequences from the Chinese and Korean specimens settled down in another group (Continental type). We have isolated the active type of transposable element (Bmamat1) from the locus of BmTNML of Korean and Japanese mandarina. Vitellogenin genes of B. mandarina are closer to B. mori than other wild silk moth.

本研究通过对分布于东亚地区的天蚕等几种野生蚕蛾的部分基因或DNA标记区域的比较，分析了它们在分子水平上的多态性。B。在B中，其突变体由于缺乏或缺乏尿酸在皮肤下的积累而导致半透明皮肤。森。日本对虾的Og基因种类繁多，与B组不同。森。我们对B的线粒体coxl基因进行了分析。Mori，并发现相对于OG基因的序列多样性水平低。碱性磷酸酶（Alp）基因在B中串联复制。桑。Alp基因（Alp-m、Alp-s）在核苷酸序列上约有60%的同源性，但间插区域的结构高度多态，在菌株之间存在大的插入/缺失。在这项研究中，我们发现Alp基因在B中是重复的。与B中的相似。森，在所有四个宝石。B中的介入区域的结构。B.B.B.提出，目前家蚕品系与野生B的最近共同祖先。在中国，有一种叫“中国人”的东西。从分析Cecropia-ITR-MLE，大多数MLE序列来自B。在日本本岛的古生物组成了一个独特的群（日本型）;另一方面，来自中国和朝鲜标本的序列定居在另一个群（大陆型）。我们已经从韩国和日本的BmTNML基因座中分离出了转座因子（Bmamat 1）的活性类型。B的卵黄原蛋白基因。女同性恋更接近B。桑蚕蛾比其他野生蚕蛾多。

项目成果

Sequence Comparison of mariner-like elements among the populations of Bombyx mandarina inhabiting China, Korea and Japan

中、韩、日三国家蚕种群水手样元件序列比较

• 期刊: J. Insect Biotechnology and Sericology accepted
• 作者: Kawanishi;Y.;Takaishi;R.;Banno;Y.;Fujimoto;H.;Nho;S-K;Maekawa;H;Nakajima;Y.
• 通讯作者: Y.

FISH analysis of the W chromosome in Bombyx mandarina and several other species of Lepidontera by means of B. mori W-BAC probes. 6. Meng, Y., Liu, C, Shiomi, K., Nakagaki, M.

利用家蚕 W-BAC 探针对家蚕和其他几种鳞翅目昆虫的 W 染色体进行 FISH 分析。

• 发表时间: 2007
• 期刊: J. of Insect Biotechnology and Sericology 76 (1)
• 作者: Yoshido;A.;Yasukochi;Y;Marec;E;Abe;H.;Sahara;K.
• 通讯作者: K.

Sequence Comparison of mariner-like-elements and inserted sequences into them among Bombyx mandarina inhabiting in China, Korea and Japan. (Invited speaker)

中国、韩国和日本的家蚕水手样元件及其插入序列的序列比较。

• 发表时间: 2005
• 作者: Kawanishi;Y.;Takaishi;R.;Banno;Y.;Fujimoto;H.;Maekawa;H.;Nakaiima. Y.
• 通讯作者: Nakaiima. Y.

A novel maT-type transposable element, BmamaT1, in Bombyx mandarina, homologous to the B. mori mariner-like element Bmmar6.

家蚕中一种新的 maT 型转座元件 BmamaT1，与家蚕水手样元件 Bmmar6 同源。

• 发表时间: 2008
• 期刊: Journal of Insect Biotechnology and Sericology 77(1)
• 作者: Kawanishi;Y.;Morimoto;M.;Banno;Y.;Nho;S. K.;Maekawa;H.;and Nakajima;Y.
• 通讯作者: Y.

Method for rapid distinction of Bombyx mandarina (Japan) from B. mamdarina (China) based on rDNA sequence difference.

基于rDNA序列差异快速区分日本家蚕和中国家蚕的方法

• 发表时间: 2006
• 期刊: Journal of Insect Biotechnology and Sericology 77(2)(in press)
• 作者: Kawanishi;Y.;Banno;Y.;Fujimoto;H.;Nho;S. K.;Tu;Z.;Mita;K.;Tshuchida;K.;Takada;N.;Maekawa;H.;and Nakajima;Y.
• 通讯作者: Y.