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Influence of phytoestrogens on development of reproductive functions

植物雌激素对生殖功能发育的影响

基本信息

批准号：
17570052
负责人：
YAMANOCHI Korehito
金额：
$ 2.39万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

In this experiment, effects of phytoestrogens, coumestrol, genistein and resveratrol, on sexual differentiation and reproductive behaviors were examined in the rat.1. Effects of phytoestrogens on masculinization of the brain functions(1) Effects of coumestrol(CM) : Single injection with 3mg CM on day 5 after birth induced acceleration of vaginal opening, acyclic change in vaginial smear, small ovaries without corpus luteum and low lordotic activity in female rats, as injection with 1mg estradiol. On the other hand, no effects were observed when injected with genistein.(2) Effects of resveratrol(RTL) : Single injection with 5mg RTL on day 5 after birth induced acyclic change in vaginal smear in a few rats, but not in most female rats. Lordotic activity was not influenced by injection with RTL.(3) Prevention of masculinizing effects of coumestrol by progesterone : Progesterone is known to suppress functions of estrogen in the brain. In this experiment, simultaneous injection with 3mg CM and 0.5mg progesterone to female pups on day 5 after birth induced no effect on vaginal opening, vaginal smear and lordotic activity. This indicates that progesterone also can prevent masculinizing effect of phytoestrogens.2. Effects of phytoestrogens on lordosis and maternal behavior in adult female rats.(1) Sexual behavior : Ovariectomized rats were injected with 1mg CM and 0.5mg progesterone 44 hrs after injection of CM and lordosis behavior was observed. As a result no effects were observed. No effects were observed even when injected those in ovariectomized rats treated of substhresthold dose of estrogen.(2) Maternal behavior : Injections with 1mg estradiol benzoate daily for 10 days accelerated onset of maternal behavior but lmg CM in ovariectomized rats did not.These results indicate that phytoestrogen is effective in mascuilinizing the brain in the neonatal period but not in facilitation of reproductive behaviors in adult female rats.

本实验研究了植物雌激素香豆雌酚、染料木素和白藜芦醇对大鼠性分化和生殖行为的影响.植物雌激素对脑功能雄性化的影响（1）香豆雌酚（CM）的作用：生后第5天单次注射CM 3mg，可引起雌性大鼠阴道张开速度加快，阴道涂片无周期性改变，卵巢变小，无黄体，脊柱前凸活动减弱，与注射雌二醇1mg相似。另一方面，当注射染料木黄酮时没有观察到效果。(2)白藜芦醇（RTL）的影响：出生后第5天单次注射5mg RTL，在少数大鼠中诱导阴道涂片的非周期性变化，但在大多数雌性大鼠中没有。前凸活动不受RTL注射的影响。(3)黄体酮预防香豆雌酚的男性化作用：众所周知，黄体酮可以抑制大脑中雌激素的功能。在本实验中，在出生后第5天向雌性幼崽同时注射3mg CM和0.5mg孕酮对阴道开口、阴道涂片和脊柱前凸活动没有影响。这表明孕酮也能阻止植物雌激素的雄性化作用.植物雌激素对成年雌性大鼠脊柱前凸和母性行为的影响。(1)性行为：去卵巢大鼠注射CM 1mg，44小时后注射孕酮0.5mg，观察大鼠脊柱前凸行为。因此，未观察到任何影响。即使在卵巢切除大鼠中注射亚阈值剂量的雌激素，也没有观察到任何影响。(2)母性行为：每天注射苯甲酸雌二醇1mg，连续10天，可加速去卵巢大鼠的母性行为，而CM 1mg则无此作用，表明植物雌激素对新生期大鼠的大脑有雄性化作用，但对成年雌性大鼠的生殖行为无促进作用。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

ラット脳におけるエストロゲンα受容体免疫陽性細胞数の部位差と雌雄差

大鼠脑内雌激素α受体免疫阳性细胞数量的区域性和男女性差异

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Kishitake;M.;Yamanouchi;K.;山内兄人;山内兄人;山田俊児・和田静子・山内兄人;山内兄人;山内兄人;伊藤広幸・野口大輔・山田俊児・山内兄人
通讯作者：
伊藤広幸・野口大輔・山田俊児・山内兄人

Maternal exposure to a low dose dioxin affects the paired associative learing behavior of offspring of Long-Evans rats

母亲接触低剂量二恶英会影响 Long-Evans 大鼠后代的配对联想学习行为

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Kakeyama;M.;Endo T.;Yamanouchi K;Tohyama C.
通讯作者：
Tohyama C.

Effects of estrogen,androgen or phytoestrogen on retrieving and licking behaviors in nulliparous and male rats

雌激素、雄激素或植物雌激素对未产及雄性大鼠检索和舔舐行为的影响

DOI：
发表时间：
2007
期刊：
Zool.Sci. 24
影响因子：
0
作者：
Mariko Sakai;Korehito Yamanouchi
通讯作者：
Korehito Yamanouchi

Effiects of estrogen,androgen or phytoestrogen on retrieving and licking behaviors in nulliparous and male rats

雌激素、雄激素或植物雌激素对未产及雄性大鼠检索和舔舐行为的影响

DOI：
发表时间：
2007
期刊：
Zoolgical Science 24
影响因子：
0
作者：
Sakai;M. ;Yamanouchi;K.;Tom Kouki and Korehito Yamanouchi;Mariko Sakai and Korehito Yamanouchi
通讯作者：
Mariko Sakai and Korehito Yamanouchi