Studies on the relationship between genetic diversity within the fungus that specifically occur on Fagus crenata supule and its host distribution.

水青冈特异真菌遗传多样性与其寄主分布关系的研究。

• 批准号: 17570088
• 负责人: HOSOYA Tsuyoshi
• 金额: $ 2.43万
• 依托单位: National Museum of Nature and Science, Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17570088/
• 关键词: Fagus crenata; Dasyscyphella longistipitata; geographical isolation; genetic diversity; ITS-5.8S ribosomal DNA gene; haplotype; ascomycete; distribution; UPGMA

To search for major factors that affected fungal distribution, ITS sequence diversity of Dasyscyphella longistipitata(Hyaloscyphaceae, Helotiales, Disomyoate, Ascomycota) that occur on Fagus crenata cupulus obtained from wide areas in Japan were compared. Five to 20 cupules were collected every 5m apart at each sampling site, and single-spored isolates were obtained. In 120 isolates obtained from 12 sites, 38 haplotypes were detected, and the haplotype H12(43% of the total isolates) was detected from all the populations. In addition, H28(15%) and H10(10%) consisted of the three major haplotypes. These three haplotypes were placed in inner clades in the haplotype network based on statistical parsimony. Nested clade analysis did not indicate geographic isolation. Analysis of molecular variance attributed the genetic diversity for the diversity within the population. Genetic distance was not found to be correlated with geological distance, and no relationship was found with the phylogenetic grouping of the host. These facts suggested the whole the populations of D.longistipitata Japan form a large continuum. However, genetic diversity was higher in south-western areas and north eastern areas in Japan. This genetic distribution pattern suggests that the current population initiated from the ancestral population in the glacier period with high genetic diversity in south-western Japan, followed by the continuous expansion along with the host expansion, decrease of the genetic diversity in the middle Japan due to the extinction of the host, while keeping diversity due to continuous distribution of the host in the north-east Japan.

为了寻找影响真菌分布的主要因素，比较了日本广泛地区产自牛油果（Fagus crenatata cupulus）上生长的长鞭毛虫（Dasyscyphella longistipitata）的ITS序列多样性。每个采样点每隔5m采集5 ~ 20个小孢子，获得单孢子分离株。在12个地点获得的120株分离株中，检测到38个单倍型，其中H12单倍型在所有群体中检测到，占总分离株的43%。此外，H28（15%）和H10（10%）由三种主要单倍型组成。根据统计简约性将这三个单倍型置于单倍型网络的内支。嵌套进化支分析未显示地理隔离。分子变异分析将遗传多样性归因于群体内的多样性。遗传距离与地质距离无相关性，与宿主的系统发育类群无相关性。这些事实表明，日本长纹田鼠的整个种群形成了一个大的连续体。而日本西南部和东北部地区的遗传多样性较高。这一遗传分布格局表明，当前种群起源于日本西南部具有较高遗传多样性的冰川时期祖先种群，随后随着寄主扩张而不断扩张，日本中部因寄主灭绝而遗传多样性下降，而日本东北部因寄主持续分布而保持多样性。

项目成果

ブナ殻斗に発生する盤菌類Dasyscyphella longistipitataの地理的分布

山毛榉冠层圆盘真菌 Dasyscyphella longistipitata 的地理分布

• 发表时间: 2007
• 作者: Nakamura;K.;R. Suwa;T. Denda;M. Yokota;横田昌嗣・他26名;横田昌嗣;藤崎みちる・細矢剛・柿嶌眞
• 通讯作者: 藤崎みちる・細矢剛・柿嶌眞

ブナ殻斗に発生する菌類・Dasyyscyphella longistipttata(盤菌類、ビョウタケ目、ラクヌム科)の系統地理学的分布

山毛榉树冠中真菌 Dasyyscyphella longistipttata（Dischimycota、Bacteriales、Lacnumidae）的系统发育地理分布

• 发表时间: 2007
• 作者: Nakamura;K.;R. Suwa;T. Denda;M. Yokota;横田昌嗣・他26名;横田昌嗣;藤崎みちる・細矢剛・柿嶌眞;藤崎みちる・細矢剛・広瀬大・窪野高徳・大園享・柿嶌眞;藤崎 みちる・細矢 剛・広瀬 大・窪野 高徳・大園 享・柿嶌 眞
• 通讯作者: 藤崎 みちる・細矢 剛・広瀬 大・窪野 高徳・大園 享・柿嶌 眞

ブナ殻斗に発生する菌類Dasyscyphella longistipitata(盤菌類、ビョウタケ目、ラクヌム科)の系統地理学的分布

山毛榉冠层真菌 Dasyscyphella longistipitata（Dischimycota、Bacteriales、Lacnumidae）的系统发育地理分布。

• 发表时间: 2007
• 作者: Nakamura;K.;R. Suwa;T. Denda;M. Yokota;横田昌嗣・他26名;横田昌嗣;藤崎みちる・細矢剛・柿嶌眞;藤崎みちる・細矢剛・広瀬大・窪野高徳・大園享・柿嶌眞
• 通讯作者: 藤崎みちる・細矢剛・広瀬大・窪野高徳・大園享・柿嶌眞