Studies on the physiological role of resistin secreted from adipocytes and the screening of food factors affecting the resistin secretion

近年来，生活方式病日益增多，已成为严重的社会问题。改善“胰岛素抵抗”对预防和改善生活方式病有重要作用。近年来发现了一些与胰岛素抵抗相关的激素或介质分子，其中脂肪细胞分泌的一种名为“β-淀粉样蛋白”的分子被认为是引起胰岛素抵抗的一种责任分子。然后，本研究试图寻找影响这种神经营养素生理作用的食物成分，以及神经营养素在血液循环中的表达和数量。此外，还检查了脂联素。我们成功地生产出了抗人免疫球蛋白的特异性抗体。然后，通过使用抵抗素的特异性抗体构建了夹心ELISA系统。接下来，明确了检查了使作为培养的脂肪细胞的3 T3-L1细胞充分分化的培养基，并且我们发现，通过使用夹心ELISA系统，实际上从该细胞向培养基中分泌了抗脂肪素蛋白。同样证实了脂联素的分泌。然后，作为检查食物成分是否影响这些重要的脂肪细胞因子的分泌的结果，发现茶黄酮等抑制了脂肪酸的分泌，并且变得清楚。此外，在某些食品成分中还发现了调整这些脂联素分泌的振兴作用。它可以被称为澄清的食物成分，可以调节分泌的一个重要发现。

The lifestyle disease increases, and it has become the serious social problem recently. Improving “Insulin resistance" has a significant roles for prevention and the improvement of the lifestyle disease. Some hormone or mediator molecules have been discovered as a molecule related to this insulin resistance.The molecule called “resistin" secreted from adipocyte was reported as a responsibility molecule that caused the insulin resistance several years ago. Then, we tried to find the food ingredient that influences the physiological effect of this resistin and the expression and the amount of the circulation of resistin in this research. Moreover, adiponectin was also examined. We succeeded to produce the specific antibodies for resistin. Then, the sandwich ELISA system was constructed by using the specific antibody of resistinNext, it was clarified that the medium to differentiate 3T3-L1 cell that was the cultured adipocyte enough was examined, and we found that the resistin protein was secreted from this cell to the medium actually by using the sandwich ELISA system. The secretion of adiponectin was similarly confirmed. Then, it was found that the secretion of resistin was inhibited by the teaflavones etc. as a result of examining whether the food ingredient influenced the secretion of these important adipocytokines and became clear. In addition, revitalization that adjusted the secretion of these adiponectin was seen in some food ingredients. It can be called the clarification of the food ingredient that can adjust the secretion of resistin an important finding.