Studies on the physiological role of resistin secreted from adipocytes and the screening of food factors affecting the resistin secretion

脂肪细胞分泌抵抗素的生理作用研究及影响抵抗素分泌的食物因素筛选

• 批准号: 17580109
• 负责人: MORIYAMA Tatsuya
• 金额: $ 2.35万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17580109/
• 关键词: resistin; adipocytokine; adipose tissue; adiponectin; ELISA; 3T3-L1; 分泌; リポタンパク質; 食品成分; 炎症; 高脂肪食; 発現変動; マウス; インスリン抵抗性; ウエスタンブロット

The lifestyle disease increases, and it has become the serious social problem recently. Improving “Insulin resistance" has a significant roles for prevention and the improvement of the lifestyle disease. Some hormone or mediator molecules have been discovered as a molecule related to this insulin resistance.The molecule called “resistin" secreted from adipocyte was reported as a responsibility molecule that caused the insulin resistance several years ago. Then, we tried to find the food ingredient that influences the physiological effect of this resistin and the expression and the amount of the circulation of resistin in this research. Moreover, adiponectin was also examined. We succeeded to produce the specific antibodies for resistin. Then, the sandwich ELISA system was constructed by using the specific antibody of resistinNext, it was clarified that the medium to differentiate 3T3-L1 cell that was the cultured adipocyte enough was examined, and we found that the resistin protein was secreted from this cell to the medium actually by using the sandwich ELISA system. The secretion of adiponectin was similarly confirmed. Then, it was found that the secretion of resistin was inhibited by the teaflavones etc. as a result of examining whether the food ingredient influenced the secretion of these important adipocytokines and became clear. In addition, revitalization that adjusted the secretion of these adiponectin was seen in some food ingredients. It can be called the clarification of the food ingredient that can adjust the secretion of resistin an important finding.

生活方式病增多，已成为近年来严重的社会问题。改善“胰岛素抵抗”对于预防和改善生活习惯病具有重要作用。一些激素或介质分子已被发现作为与胰岛素抵抗相关的分子。几年前，脂肪细胞分泌的称为“抵抗素”的分子被报道为导致胰岛素抵抗的责任分子。然后，本研究试图寻找影响这种抵抗素生理作用的食品成分以及抵抗素的表达和循环量。此外，还检查了脂联素。我们成功生产了抵抗素的特异性抗体。然后，使用抵抗素特异性抗体构建夹心ELISA系统，明确了对分化3T3-L1细胞（即培养的脂肪细胞）的培养基进行了足够的检查，并且我们发现通过使用夹心ELISA系统，抵抗素蛋白实际上从该细胞分泌到培养基中。类似地证实了脂联素的分泌。然后，通过检查食品成分是否影响这些重要的脂肪细胞因子的分泌，结果发现抵抗素的分泌被茶黄酮等抑制，并且变得清楚。此外，在一些食品成分中还发现了调节这些脂联素分泌的活化作用。这堪称调节抵抗素分泌的食品成分的一项重要发现。

项目成果

肥満の分子メカニズムとその制御

肥胖的分子机制及其控制

• 发表时间: 2005
• 期刊: 科学と工業 79・4
• 作者: Akihiko Nagao;Megumi Hase;Kenichi Otsubo;森山達哉
• 通讯作者: 森山達哉

全身性炎症状態におけるレジスチンレベルの変動解析

全身炎症条件下抵抗素水平变化的分析

• 发表时间: 2007
• 作者: 牧野 泰子;各務 健太;松村 崇史;森山 達哉;河村 幸雄
• 通讯作者: 河村 幸雄

adipose tissues and adipocytokines(in Japanese)

脂肪组织和脂肪细胞因子（日语）

• 发表时间: 2005
• 作者: Tatsuya Moriyama;Teruo Kawada
• 通讯作者: Teruo Kawada