Analysis of changes of pollenosis-related class-2 allergen levels in major agricultural products.

主要的泛过敏原，Betv1同源物，profilin，和奇异果甜蛋白样蛋白，克隆和细菌中表达。将获得的重组变应原蛋白用于抗体生产。所产生的抗体用于分析这些花粉症相关的2类过敏原水平的变化，在主要农产品。这些过敏原水平因物种、培养方法、加工方法、烹饪方法的变化而发生剧烈变化。此外，还测定了各种农产品中的致敏原。

The major pan-allergens, Betv1 homolog, profilin,and thaumatin-like protein, were cloned and expressed in bacteria. Obtained recombinant allergen proteins were used for antibody production. Produced antibodies were used for the analysis of changes of these pollenosis-related class 2 allergen levels in major agricultural products. These allergen levels were drastically changed for the variation of species, methods of culture, processing methods, cooking methods. In addition, causative allergens were determined in the various agricultual products.