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Expression profiling of C1 metabolic genes involved in monolignol biosynthesis (2007)

参与木质素生物合成的 C1 代谢基因的表达谱 (2007)

基本信息

批准号：
17580145
负责人：
KAJITA Shinya
金额：
$ 2.42万
依托单位：
Tokyo University of Agriculture and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17580145/
关键词：
C1 metabolism gene profiling lignin guaiacyl lignin syringyl lignin 細胞壁木化

项目摘要

In plant cell, some C1 metabolic genes play important role in supply of methyl residues for synthesis of various types of compounds. We tried to identify which genes in the C1 metabolic pathway are important for lignin biosynthesis in which a lot of methyl residues should be needed. Expression of several genes, serine hydroxymethyltrans-ferase (SHMT), glycine decarboxylase, formyl THF synthetase, THF dehydrogenase/cyclohydrolase, Methylene THF reductase, Methionine synthetase, Methionine adenosyl-transferase, were analyzed by RT-PCR and virtual Northern blot analyses. In poplar plants, one of SHMT genes is expressed in xylem tissue which contains higher amount of lignin. In Arabidopsis, SHMTs were coded by seven different genes. At first, we analyzed expression pattern of each genes in various tissue of Arabidopsis plant by PT-PCR. As a result of the analysis, although six of the seven genes were expressed in xylem tissue, expression of SHMT7 (At4g13930) was highest among those of the genes analyzed. For more depth analysis, we isolated Arabidopsis mutant with T-DNA insertion into one of SHMT genes (SHMT7, At4g13930) and characterized phenotypes of it. After cultivation of the mutant in soil, lignin biosynthesis of the plant was analyzed under light microscopy with thin section of stem tissue. The results of the analysis suggest lignin content of xylem tissue in the mutant is slightly lower than that in wild-type plant. We analyzed. These data indicate that SHMT may play any role in supply of methyl residue to lignin biosynthetic pathway.

在植物细胞中，一些C1代谢基因在为各种化合物的合成提供甲基残基方面起着重要作用。我们试图确定C1代谢途径中哪些基因对木质素生物合成很重要，其中需要大量的甲基残基。用RT-PCR和虚拟北方杂交分析了丝氨酸羟甲基转移酶（SHMT）、甘氨酸脱羧酶、甲酰四氢呋喃合成酶、四氢呋喃脱氢酶/环化水解酶、亚甲基四氢呋喃还原酶、甲硫氨酸合成酶、甲硫氨酸腺苷转移酶等基因的表达。在白杨中，SHMT基因在木质素含量较高的木质部组织中表达。在拟南芥中，SHMT由七个不同的基因编码。首先，我们利用PT-PCR技术分析了每个基因在拟南芥不同组织中的表达模式。分析的结果是，虽然7个基因中的6个在木质部组织中表达，但SHMT 7（At 4g 13930）的表达在所分析的基因中最高。为了更深入地研究拟南芥中的木质素合成，我们分离了SHMT基因的T-DNA插入突变体（SHMT 7，At 4g 13930），并对其表型进行了表征。分析结果表明，突变体木质部组织中的木质素含量略低于野生型。我们分析了。这些数据表明，SHMT可能发挥任何作用，在供应甲基残留的木质素生物合成途径。