Development of high-throughput gene profiling system to monitor genetic background for mouse strains

认为遗传背景的不均一性对表型分析有影响。本研究采用TaqMan分析技术构建了高通量基因谱分析系统，该系统采用384孔板设计，包含192个标记（包括184个SNP和8个转基因）。该系统可以从SNPs标记中获得诸如替换与回交品系的比率、B6/J和B6/N的每个B6亚系和生产育种者的详细差异、父系或母系来源以及作为基因修饰的转基因（即neo、cre和Gfp等）的存在等信息。从这些检测标记。我们开发了一种工具，可以将原始基因分型数据转换为菌株分布模式，从而有效地检测详细的遗传背景。此外，我们还改进了利用SNPs标记在B6亚株之间进行有效基因定位的方法。

It was considered that there was affection of ununiformed genetic background of the mouse on analyzing its phenotyping. We constructed the high-throughput gene profiling system for the evaluation of genetic background configured with 192 markers (consisted of 184 SNPs and 8 transgenes) using 384 well plate format based on TaqMan assay technologies. This system was possible to get information such as the ratio of replacement to backcrossing strain, the detailed difference in each B6 substrains and production breeders of B6/J and B6/N and the paternal or maternal origin from SNPs markers and the existence of transgenes as gene modification (i.e. neo, cre, and Gfp etc.) from these detection markers. We developed tool to convert the table from the raw genotyping data to the strain distribution patterns and enabled effective to detection detailed genetic background. In addition, we have improved to effective gene mapping among B6 substrains by using SNPs markers.