Follow-up study of the association of lifestyles with chromosome DNA stability in peripheral leukocytes

这项研究旨在跟踪约200名健康的日本男性员工的生活方式和外周血白细胞的DNA稳定性。经过3年的跟踪研究，研究结果总结如下：2005年202人，2006年165人，2007年182人，年龄20~60岁。每名受试者填写一份与吸烟、饮酒、工作时间、营养平衡等生活方式相关的问卷。问卷发放后两周收集问卷时，采集外周血5~10ml。然后进行血液生化检测、彗星试验和DNA提取。检测XRCC1、ALDH2、CYP1A1、GSTM1和GSTT1基因的多态性。本研究由大阪大学伦理委员会批准，134名受试者完成了为期三年的追踪研究。他们的生活方式由u…进行评估森本保健实践指数(HPI)得分较高，HPI总分为4.86±1.36。此外，2005年、2006年和2007年的HPI分别为4.87±1.36、4.87±1.37和4.85±1.35。统计分析表明，在这三年中，生活方式没有显著变化(p&gt；0.05)。同时，在这三年中，生活方式的改变和彗星试验检测到的外周血白细胞DNA稳定性之间没有统计学上的相关性。但是，暴露在香烟焦油中的水平、工作时间和饮酒频率被证明与外周血白细胞中的暂时性DNA损伤在统计学上相关。同时，吸烟者的白细胞计数明显高于不吸烟者。ALDH2基因多态性与饮酒频率显著相关。在将饮酒者分为ALDH2充足组和ALDH2缺乏组后，饮酒频率与彗星参数呈显著负相关。综上所述，本研究表明，尽管彗星试验检测到饮酒者的生活方式与一过性DNA损伤有关，但所调查的三年中生活方式的变化与彗星试验检测到的外周血白细胞DNA稳定性没有显著关联。较少

This study was designed to follow lifestyles and DNA stability of peripheral blood leukocytes of about 200 healthy Japanese male employees. After three years of follow-up study, the results were summarized as follows.During the period of the study, 202 subjects in 2005, 165 subjects in 2006 and 182 subjects in 2007 aged 20-60 years old participated the investigation with an informed consent. Each subject filled a questionnaire relating to lifestyles such as cigarette smoking, alcohol drinking, working hours and nutrition balance etc. When the questionnaires were collected two weeks later after questionnaire distribution, 5-10 ml of peripheral blood were collected. Blood biochemical measurements, comet assay and DNA extraction were then conducted. The polymorphisms of XRCC1, ALDH2, CYP1A1, GSTM1 and GSTT1 were detected. This study was approved by the Ethics Committee of Osaka University.There were 134 subjects completed the three-year follow-up study. Their lifestyles were assessed by u … More sing Morimoto Health Practice Index (HPI), and the total average of HPI was 4.86 ± 1.36. In addition, the HPI was 4.87 ± 1.36, 4.87 ± 1.37, and 4.85 ± 1.35 for year 2005, 2006, and 2007, respectively. Statistical analysis showed that there was not a significant lifestyles change during these three years (p > 0.05). In the meantime, no statistical correlations between lifestyle change and DNA stability in the peripheral blood leukocyte as detected by comet assay was detected during the three years.However, levels of exposure to cigarette tar, working hours and alcohol drinking frequency were demonstrated to statistically correlate with a temporary DNA damage in the peripheral blood leukocyte. In the meantime, the white cell count in smokers was significantly higher than that in the nonsmokers. ALDH2 polymorphisms were significantly associated with alcohol drinking frequencies. After categorizing alcohol drinkers into ALDH2-sufficient and ALDH2-deficient groups, the drinking frequency was negatively significantly associated with the comet parameters in the present study.Summarily, the present study suggests that, wile the lifestyle showed an association with a temporary DNA damage as detected by comet assay, the investigated lifestyle change during the three-years failed to show a significant association with the DNA stability in peripheral blood leukocytes as detected by comet assay. Less