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Follow-up study of the association of lifestyles with chromosome DNA stability in peripheral leukocytes

生活方式与外周血白细胞染色体DNA稳定性关系的随访研究

基本信息

批准号：
17590513
负责人：
LU Yuquan
金额：
$ 2.2万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17590513/
关键词：
Lifestyle Health Practice Index Comet Assay DNA stability Peripheral blood leukocyte Enidemioloeical investieation Single-cell gel electrophoresis Single-cell gel electrophoresis Granulysin Health Pract ice Index

项目摘要

This study was designed to follow lifestyles and DNA stability of peripheral blood leukocytes of about 200 healthy Japanese male employees. After three years of follow-up study, the results were summarized as follows.During the period of the study, 202 subjects in 2005, 165 subjects in 2006 and 182 subjects in 2007 aged 20-60 years old participated the investigation with an informed consent. Each subject filled a questionnaire relating to lifestyles such as cigarette smoking, alcohol drinking, working hours and nutrition balance etc. When the questionnaires were collected two weeks later after questionnaire distribution, 5-10 ml of peripheral blood were collected. Blood biochemical measurements, comet assay and DNA extraction were then conducted. The polymorphisms of XRCC1, ALDH2, CYP1A1, GSTM1 and GSTT1 were detected. This study was approved by the Ethics Committee of Osaka University.There were 134 subjects completed the three-year follow-up study. Their lifestyles were assessed by u … More sing Morimoto Health Practice Index (HPI), and the total average of HPI was 4.86 ± 1.36. In addition, the HPI was 4.87 ± 1.36, 4.87 ± 1.37, and 4.85 ± 1.35 for year 2005, 2006, and 2007, respectively. Statistical analysis showed that there was not a significant lifestyles change during these three years (p > 0.05). In the meantime, no statistical correlations between lifestyle change and DNA stability in the peripheral blood leukocyte as detected by comet assay was detected during the three years.However, levels of exposure to cigarette tar, working hours and alcohol drinking frequency were demonstrated to statistically correlate with a temporary DNA damage in the peripheral blood leukocyte. In the meantime, the white cell count in smokers was significantly higher than that in the nonsmokers. ALDH2 polymorphisms were significantly associated with alcohol drinking frequencies. After categorizing alcohol drinkers into ALDH2-sufficient and ALDH2-deficient groups, the drinking frequency was negatively significantly associated with the comet parameters in the present study.Summarily, the present study suggests that, wile the lifestyle showed an association with a temporary DNA damage as detected by comet assay, the investigated lifestyle change during the three-years failed to show a significant association with the DNA stability in peripheral blood leukocytes as detected by comet assay. Less

本研究旨在追踪约 200 名健康日本男性员工的生活方式和外周血白细胞 DNA 稳定性。经过三年的跟踪研究，结果总结如下。研究期间，2005年202名受试者、2006年165名受试者165名和2007年182名受试者年龄20-60岁在知情同意的情况下参加了调查。每位受试者填写一份与吸烟、饮酒、工作时间、营养平衡等生活方式相关的问卷。问卷发放两周后回收问卷时，采集外周血5-10ml。然后进行血液生化测量、彗星试验和DNA提取。检测XRCC1、ALDH2、CYP1A1、GSTM1、GSTT1多态性。本研究经大阪大学伦理委员会批准。共有134名受试者完成了为期三年的随访研究。他们的生活方式通过森本健康实践指数（HPI）进行评估，HPI 的总平均值为 4.86 ± 1.36。此外，2005年、2006年和2007年的HPI分别为4.87±1.36、4.87±1.37和4.85±1.35。统计分析显示，这三年间生活方式没有显着改变（p > 0.05）。与此同时，三年内生活方式的改变与彗星试验检测到的外周血白细胞DNA稳定性之间没有发现统计相关性。然而，烟焦油暴露水平、工作时间和饮酒频率被证明与外周血白细胞中暂时性DNA损伤存在统计相关性。与此同时，吸烟者的白细胞计数明显高于不吸烟者。 ALDH2 多态性与饮酒频率显着相关。将饮酒者分为 ALDH2 充足组和 ALDH2 缺乏组后，在本研究中饮酒频率与彗星参数呈显着负相关。总之，本研究表明，虽然生活方式显示出彗星试验检测到的暂时性 DNA 损伤相关，但所调查的三年期间生活方式的变化未能显示出与外周血 DNA 稳定性的显着相关性。通过彗星试验检测白细胞。较少的