权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Research on the effect of endocrine-disrupting chemicals on the function of reproduction and central nervous system during fetal and neonatal life

内分泌干扰物对胎儿和新生儿生命过程中生殖和中枢神经系统功能影响的研究

基本信息

批准号：
17591138
负责人：
KAMEI Yoshimasa
金额：
$ 2.37万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

Glutamate-induced neuronal cell death has been implicated in the pathogenesis of neonatal hypoxic-ischemic brain injury and other neurodegenerative diseases. To investigate the possibility of a new neuroprotection strategy targeting Bax translocation in glutamate-induced neuronal cell death, cerebellar granule neurons (CGNs) were exposed to glutamate with or without BIP, a novel membrane-permeable peptide which can bind Bax in the cytosol and inhibit its translocation to the mitochondria. Pretreatment of CGNs with BIP elicited a dose-dependent reduction of glutamate-induced neuronal cell death as measured by the MTT assay. BIP significantly suppressed both the number of TUNEL-positive cells and the increase in caspase 3 and caspase 9 activities induced by glutamate. In addition, immunoblots after subcellular fractionation revealed that BIP prevented the glutamate-induced Bax translocation to the mitochondria and the release of cytochrome c from the mitochondria. These results suggest that agents capable of inhibiting Bax activity such as BIP might lead to new drugs for glutamate-related diseases in the future.Oligodendrocytes (Ols) have been implicated to be the main player in the development of periventricular leukomalacia in the perinatal period. Recently, hypothermia therapy for the neonates with hypoxic-ischemic brain injurues has been reported to be effective for the protection of the neurological disabilities. We investigated the mechanism of the hypothermia on the OL function using primary culture of Ols. We found proliferation of Ols is at maximum at lower temperature and DNA microarray analysis proved that many genes are involved in this process. We will further analyze the mechanism of the proliferation of Ols.

谷氨酸诱导的神经元细胞死亡与新生儿缺氧缺血性脑损伤和其他神经退行性疾病的发病机制有关。为了研究一种新的神经保护策略的可能性，在谷氨酸诱导的神经元细胞死亡中靶向Bax易位，小脑颗粒神经元（CGNs）暴露于谷氨酸，有或没有BIP，一种新的膜渗透性肽，可以结合Bax在胞质溶胶中，并抑制其易位到线粒体。用BIP预处理CGN引起谷氨酸诱导的神经元细胞死亡的剂量依赖性减少，如通过MTT测定所测量的。BIP显著抑制谷氨酸诱导的TUNEL阳性细胞数和caspase 3和caspase 9活性的增加。此外，亚细胞分级分离后的免疫印迹显示，BIP阻止谷氨酸诱导的Bax易位到线粒体和细胞色素c从线粒体的释放。这些结果表明，能够抑制Bax活性的药物，如BIP，可能会导致新的药物，谷氨酸相关疾病的未来。少突胶质细胞（Ols）已被牵连是在围产期脑室周围白质软化症的发展的主要球员。近年来，亚低温治疗新生儿缺氧缺血性脑损伤被认为是一种有效的神经功能保护方法。本研究采用原代培养的OLs，探讨低温对OLs功能的影响机制。我们发现Ols在低温下增殖最快，DNA芯片分析证实了这一过程涉及到多个基因。我们将进一步分析Ols增殖的机制。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

炎症性腸疾患(IBD)が妊娠・分娩に及ぼす影響の検討

检查炎症性肠病（IBD）对妊娠和分娩的影响

DOI：
发表时间：
2005
期刊：
影响因子：
0
作者：
亀井良政;酒匂美奈子;北川浩明;藤井知行;上妻志郎;武谷雄二;高添正和
通讯作者：
高添正和

Changes in umbilical arterial blood flow by an intraamnitic distilled water infusion

羊膜腔内输注蒸馏水对脐动脉血流的变化

DOI：
发表时间：
2006
期刊：
J Soc Gynecol Invest 13
影响因子：
0
作者：
Komatsu A;Kozuma S;Hyodo H;Horikoshi H;Sakamaki K;Kikuchi A;Kamei Y;Fujii T;Taketani Y.
通讯作者：
Taketani Y.

胎児心疾患合併妊婦の分娩様式に関する検討

胎心病孕妇分娩方式的研究