Influences of cancer cells in the blood system on the immune system of the individuals

血液系统中的癌细胞对个体免疫系统的影响

• 批准号: 17591399
• 负责人: IKEGUCHI Masahide
• 金额: $ 2.37万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591399/
• 关键词: cancer; cancer cells in the blood system; Anti-cancer immunization; micro-metastasis; cancer recurrence; CEA mRNA; real-time RT-PCR; ooerative maninulation; CEA mRNA; リアルタイムPT-PCR

We evaluated the correlation between the postoperative detection of circulating cancer cells and the risk of recurrence in patients with gastric cancer. Total RNA was extracted from peripheral blood from 59 patients with gastric cancer and 15 patients with cholecystolithiasis (control) before and after operation. CEA mRNA positive cells were not found in the peripheral blood of the control patients either before or after operation, nor in the peripheral blood of the gastric cancer patients before operation. CEA mRNA positive cells were detected in 46% of the patients just after gastrectomy. These cancer cells disappeared from peripheral blood within 2 post-operative days. In 55 patients who underwent a curative operation, the risk for cancer recurrence (10/30; 33%) in 30 patients with CEA negative was higher than that for cancer recurrence (3/25; 12%) in 25 patients with CEA positive (p=0.064). As a result, the presence of circulating tumor cells just after surgery tends to correlate with a low rate of tumor recurrence in patients operated on for gastric cancer. These findings indicate that a gastrectomy may spread gastric cancer cells into the peripheral blood from primary tumors, however, such circulating cancer cells may be destroyed by the host immune system. At that time, the anti-cancer immunization should be established. The B16OVA (mouse melanoma cell) cells were implanted in the mice subcutaneously. After that, the B16OVA cells were injected from the mice tail vain. We evaluated the mouse anti-cancer immunization by detecting the tumor size (implanted subcutaneously), survival of mice, and the density of CD8+ T cells in the mice spleen. We found no significant difference in the tumor size, survival, and the density of CD8+ T cells between immunized mice and control. These findings may indicate that anti-tumor immunization is difficult by the circulating tumor cells in mouse.

我们评估了胃癌患者术后循环癌细胞检测与复发风险之间的相关性。从59例胃癌患者和15例胆囊结石患者手术前后外周血中提取总RNA。对照组患者手术前、手术后外周血中均未检测到癌胚抗原mRNA阳性细胞，胃癌患者手术前外周血中也未检测到癌胚抗原mRNA阳性细胞。46%的胃切除术后癌胚抗原mRNA阳性细胞。这些癌细胞在术后2天内从外周血中消失。在55例接受根治性手术的患者中，30例CEA阴性患者的癌症复发风险（10/30; 33%）高于25例CEA阳性患者的癌症复发风险（3/25; 12%）（p=0.064）。因此，手术后循环肿瘤细胞的存在往往与胃癌手术患者的低肿瘤复发率相关。这些发现表明，胃切除术可能会将胃癌细胞从原发性肿瘤扩散到外周血中，然而，这种循环癌细胞可能会被宿主免疫系统破坏。此时，应建立抗癌免疫。将B16 OVA（小鼠黑素瘤细胞）细胞皮下植入小鼠中。然后，从小鼠尾静脉注射B16 OVA细胞。我们通过检测肿瘤大小（皮下植入）、小鼠存活率和小鼠脾脏中CD 8 + T细胞密度来评估小鼠抗癌免疫。我们发现免疫小鼠和对照组之间的肿瘤大小、存活率和CD 8 + T细胞密度没有显著差异。这些结果可能表明，抗肿瘤免疫是困难的循环肿瘤细胞在小鼠。

项目成果

Prognostic Significance of RCAS1 Expression in Relation to the Infiltration of Dendritic Cells and Lymphocytes in Patients with Esophageal Carcinoma

• DOI: 10.1007/s10620-006-9408-6
• 发表时间: 2007-01
• 期刊: Digestive Diseases and Sciences
• 影响因子: 3.1
• 作者: S. Tsujitani;H. Saito;S. Oka;Teruhisa Sakamoto;S. Kanaji;S. Tatebe;M. Ikeguchi

Scirrhous gastric cancer: Therapeutic strategy.

硬质胃癌：治疗策略。

• 发表时间: 2006
• 期刊: Yonago Acta medica 49
• 作者: Ikeguchi M;et. al.
• 通讯作者: et. al.

胃癌腹膜播種転移に対するNF-κB p65 siRNAを用いた遺伝子治療の基礎的研究

NF-κB p65 siRNA基因治疗胃癌腹膜播散转移的基础研究

• 发表时间: 2006
• 作者: 池口正英;他
• 通讯作者: 他

Laparoscopic-Assisted Intraperitoneal Chemotherapy for Patients with Scirrhous Gastric Cancer

• DOI: 10.1159/000084018
• 发表时间: 2005-03
• 期刊: Chemotherapy
• 影响因子: 3.3
• 作者: M. Ikeguchi;S. Matsumoto;Shizue Yoshioka;Daiki Murakami;S. Kanaji;Shotaro Ohro;K. Yamaguchi;H. Saito;S. Tatebe;A. Kondo;S. Tsujitani;N. Kaibara

The gene expression level of transforming growth factor-β (TGF-β) as a biological prognostic marker of hepatocellular carcinoma.

转化生长因子-β（TGF-β）的基因表达水平作为肝细胞癌的生物学预后标志物。

• 发表时间: 2005
• 期刊: J Exp Clin Cancer Res 24
• 作者: Ikeguchi;M;et. al.;Ikeguchi M et al.;Ikeguchi M et al.;Ikeguchi M et al.
• 通讯作者: Ikeguchi M et al.