Intrinsic stem cell promote neuronal regeneration by oligomerization of A β 1-42monomer

内在干细胞通过 A β 1-42 单体寡聚化促进神经元再生

• 批准号: 17591513
• 负责人: KONDOH Takeshi
• 金额: $ 2.36万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591513/
• 关键词: Bone marrow stromal cells; organotopic hipocampal slice; 海馬切片培養; 幹細胞; ベータアミロイド; 骨髄間質細胞

Bone marrow stromal cells taken from EGFP transgenic mice were sorted by magnetic beads with surface markers of Sca-1 and Thy-1. The cells were then co-cultured on organotopic hippocampal slice or with neuronal cell feeder in dish. On hippocampus, both Sca-1 and Thy-1 positive cells showed 4- 8 folds higher potential to show neuron-like morphology than negative cells. In dish, negative cells fewly survived but each positive cells survived and showed neuron-like differentiation. In both culture condition, retinoic acid supplement accelerate differentiation. Differentiated Sca-1 and Thy-1 positive cells were immunohistochemically GFAP- and NeuN-negative but nestin-, neurofilament and NSE-positive. Neuron-like differentiation of bone marrow cells can be enhanced by selection using cell surface proteins.

采用表面标记为Sca-1和Thy-1的磁珠对EGFP转基因小鼠骨髓间质细胞进行分选。然后将细胞在器官置换的海马切片上或在培养皿中与神经元细胞饲养器共培养。在海马区，Sca-1和Thy-1阳性细胞呈现神经元样形态的电位均比阴性细胞高4 ~ 8倍。在培养皿中，阴性细胞很少存活，阳性细胞均存活，并呈神经元样分化。在两种培养条件下，维甲酸的补充均能促进分化。分化后的Sca-1和Thy-1阳性细胞免疫组化GFAP和neun阴性，巢蛋白、神经丝和nse阳性。骨髓细胞的神经元样分化可以通过选择细胞表面蛋白来增强。

项目成果

Carotid Artery Occlusion and Vasculature Change in C57Black/J Mice Detected by Synchrotron Radiation Microangiography

同步辐射微血管造影检测C57Black/J小鼠颈动脉闭塞和血管变化

• 发表时间: 2006
• 期刊: Kobe J Med Sci. 52(2)
• 作者: Tamaki M;Kidoguchi K;Mizobe T;Koyama J;Kondoh T;Kohmura E;Sakurai T;Yokono K;Umetani K
• 通讯作者: Umetani K

Effect of VEGF Receptor Antagonist (VGA1155) on Brain Edema in the Rat Cold Injury Model.

VEGF 受体拮抗剂 (VGA1155) 对大鼠冷损伤模型脑水肿的影响。

• 发表时间: 2007
• 期刊: Kobe J Med Sci. 53
• 作者: Koyama J;Miyake S;Sasavama T;Kondoh T;Kohmura E.
• 通讯作者: Kohmura E.

Sca-1 and Thy-1 accelerate neuron-like differentiation in bone marrow stromal cells.

• 发表时间: 2006
• 期刊: The Kobe journal of medical sciences
• 作者: Takashi Mizobe;K. Kidoguchi;M. Tamaki;T. Sasayama;T. Kondoh;E. Kohmura

Quantification of distension in rat cerebral perforating arteries

大鼠脑穿支动脉扩张的量化

• 发表时间: 2006
• 期刊: Neuroreport 17(14)
• 作者: Morishita A;Kondoh T;Sakurai T;Ikeda M;Bhattacharjee AK;Nakajima S;Kohmura E;Yokono K;Umetani K.
• 通讯作者: Umetani K.

Impaired response of perforating arteries to hypercapnia in chronic hyperglycemia

慢性高血糖时穿支动脉对高碳酸血症的反应受损

• 发表时间: 2006
• 期刊: Kobe Journal of Medical Science 52
• 作者: Sakurai;T;et. al.;Kidoguchi K;Morishita A;Oizumi XS
• 通讯作者: Oizumi XS