Immunological Control of Human Papillomavirus Infections

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1) IgG antibodies to HPV16, 52, 58 and 6 LI-Capsids and Spontaneous Regression of Cervical Intraepithelial NeoplasiaA total of 116 Japanese women with cervical intraepithelial neoplasia grade I/II (CIN I/II) were tested for cervical human papillomavirus (HPV) DNA and serum IgG antibodies to HPV16/52/58/6 L1-capsids. Our data suggest that baseline IgG reactivities to HPV L1-capsids do not serve as a predictive marker of CIN regression, in contrast to histological CIN grades and HPV DNA status.2) Interleukin-10 Promoter Polymorphism and Susceptibility to Cervical Cancer among Japanese womenA substitution of G for A at position -1082 in interleukin-10 (IL-10) promoter region is known to produce significantly higher levels of IL-10. Therefore, IL-10 promoter polymorphism may affect immunological control of oncogenic human papillomavirus (HPV) infections. To address a possible association between IL-10 promoter polymorphism and risk of cervical cancer, a total of 429 Japanese women [160 normal cytology, 186 CIN (cervical intraepithelial neoplasia) and 83 ICC (invasive cervical cancer)] were analyzed for IL-10 promoter polymorphisms. Using cellular DNA from cervical samples, we performed an ARMS-PCR method for detection of A and/or G at-1082 in IL-10 promoter region. The frequency of genotypes (G/G or G/A) associated with higher IL-10 production was 9.3% for normal cytology: 18.8% for CIN: 32.5% for ICC (P for trend <0.001). The highest IL-10 expression genotype G/G was detected in 1% of normal cytology, 1% of CIN and 6% of ICC. Our data suggest that IL-10 promoter polymorphism may contribute to the development of cervical cancer and its precursor lesions.