权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Immunological Control of Human Papillomavirus Infections

人乳头瘤病毒感染的免疫控制

基本信息

批准号：
17591722
负责人：
MATSUMOTO Koji
金额：
$ 2.37万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591722/
关键词：
human papillomavirus HPV antibody response IL-10 cervical cancer CIN ヒトパピローマウイルスサイトカイン

项目摘要

1) IgG antibodies to HPV16, 52, 58 and 6 LI-Capsids and Spontaneous Regression of Cervical Intraepithelial NeoplasiaA total of 116 Japanese women with cervical intraepithelial neoplasia grade I/II (CIN I/II) were tested for cervical human papillomavirus (HPV) DNA and serum IgG antibodies to HPV16/52/58/6 L1-capsids. Our data suggest that baseline IgG reactivities to HPV L1-capsids do not serve as a predictive marker of CIN regression, in contrast to histological CIN grades and HPV DNA status.2) Interleukin-10 Promoter Polymorphism and Susceptibility to Cervical Cancer among Japanese womenA substitution of G for A at position -1082 in interleukin-10 (IL-10) promoter region is known to produce significantly higher levels of IL-10. Therefore, IL-10 promoter polymorphism may affect immunological control of oncogenic human papillomavirus (HPV) infections. To address a possible association between IL-10 promoter polymorphism and risk of cervical cancer, a total of 429 Japanese women [160 normal cytology, 186 CIN (cervical intraepithelial neoplasia) and 83 ICC (invasive cervical cancer)] were analyzed for IL-10 promoter polymorphisms. Using cellular DNA from cervical samples, we performed an ARMS-PCR method for detection of A and/or G at-1082 in IL-10 promoter region. The frequency of genotypes (G/G or G/A) associated with higher IL-10 production was 9.3% for normal cytology: 18.8% for CIN: 32.5% for ICC (P for trend <0.001). The highest IL-10 expression genotype G/G was detected in 1% of normal cytology, 1% of CIN and 6% of ICC. Our data suggest that IL-10 promoter polymorphism may contribute to the development of cervical cancer and its precursor lesions.

1) HPV16、52、58和6 LI-衣壳的IgG抗体和宫颈上皮内瘤变的自然消退对总共116名患有宫颈上皮内瘤变I/II级(CIN I/II)的日本女性进行了宫颈人乳头瘤病毒(HPV) DNA和血清IgG抗体的检测 HPV16/52/58/6 L1-衣壳。我们的数据表明，与组织学 CIN 分级和 HPV DNA 状态相比，基线 IgG 对 HPV L1 衣壳的反应性不能作为 CIN 消退的预测标记。2) 日本女性中白细胞介素 10 启动子多态性和对宫颈癌的易感性已知白细胞介素 10 (IL-10) 启动子区域中 -1082 位的 G 取代 A 会产生显着更高水平的宫颈癌。 IL-10。因此，IL-10启动子多态性可能影响致癌人乳头瘤病毒（HPV）感染的免疫控制。为了解决 IL-10 启动子多态性与宫颈癌风险之间可能存在的关联，对总共 429 名日本女性 [160 名细胞学正常、186 名 CIN（宫颈上皮内瘤变）和 83 名 ICC（浸润性宫颈癌）] 进行了 IL-10 启动子多态性分析。使用来自宫颈样本的细胞 DNA，我们进行了 ARMS-PCR 方法来检测 IL-10 启动子区域中的 A 和/或 G at-1082。正常细胞学中与较高 IL-10 产生相关的基因型（G/G 或 G/A）频率为 9.3%：CIN 为 18.8%：ICC 为 32.5%（趋势 P <0.001）。 IL-10表达最高的基因型G/G在1%的正常细胞学、1%的CIN和6%的ICC中检测到。我们的数据表明IL-10启动子多态性可能有助于宫颈癌及其癌前病变的发展。