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Research on immunoenhancing effect of hypertonic saline resuscitation of hemorrhagic shock model in mice

高渗盐水复苏对失血性休克模型小鼠免疫增强作用的研究

基本信息

批准号：
17591892
负责人：
MURAO Yoshinori
金额：
$ 2.5万
依托单位：
Kansai Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17591892/
关键词：
hemorrhagic shock mouse hypertonic saline immunomodulatory effect CD4^+CD25^+ regulatory T cell γδ T cell CD4+CD25+制性T細胞胸腺脾臓高張食塩液

项目摘要

We examined effects of hypertonic saline resuscitation on CD4^+CD25^+ regulatory T cells and γδT cells after hemorrhagic shock and resuscitation in relation to apoptosis in mice. Methods: Male C57BL/6J mice 8 to 12 weeks old were used. Mice were anesthetized and blood was withdrawn until a mean arterial pressure of40±5mmHg was reached, and maintained for 60 min. Resuscitation was performed as follows: HS+SB; with hypertonic saline (4ml/Kg of 7.5% NaC1:HS) and shed blood (SB), 2LR+SB; with lactated Ringer' s solution (2 times the volume of the shed blood) and SB. Samples of thymus and spleen were harvested at 2,6,24 and 48h after resuscitation. CD4^+CD25^+ regulatory T cells and γδT cells were estimated using flow cytometry. Apoptosis was assessed with AnnexinV. Results: 1) Hypertonic saline resuscitation did not affect on CD4^+CD25^+ regulatory T cells and γ8T cells. 2) Regulatory T cells at 6h,24h and48h in spleen increased compared with controls. (P<0.05). 3) Apoptotic γδT cells and γδT cells both in spleen and thymus was not increased compared with controls. Discussion: Immunoenhancing effect of hypertonic saline resuscitation did not correlated with CD4^+CD25^+ regulatory T cells and γδT cells. Immunosuppressive condition after hemorrhagic shock and resuscitation may be related with CD4^+CD25^+ regulatory T cells.

我们检测了失血性休克和复苏后高渗盐水复苏对小鼠CD 4 ^+ CD 25 ^+调节性T细胞和γδT细胞凋亡的影响。方法：采用8 ~ 12周龄雄性C57 BL/6 J小鼠。小鼠麻醉后，抽血至平均动脉压为40 ± 5 mmHg，维持60分钟，复苏方法如下：HS+SB，用高渗盐水（7.5%NaCl：HS 4 ml/Kg）和放血（SB），2LR+SB;复苏后2、6、24、48 h分别取胸腺、脾脏组织，用生理盐水（2倍于失血量）灌胃。使用流式细胞术估计CD 4 ^+ CD 25 ^+调节性T细胞和γδT细胞。用AnnexinV评估细胞凋亡。结果：1）高渗盐水复苏对CD 4 ^+ CD 25 ^+调节性T细胞和γ 8 T细胞无明显影响。2)脾内调节性T细胞在6、24、48 h均较对照组增加。（P<0.05）。3)与对照组相比，脾脏和胸腺中的凋亡γδT细胞和γδT细胞均未增加。讨论：高渗盐水复苏的免疫增强作用与CD 4 ^+ CD 25 ^+调节性T细胞和γδT细胞无关。失血性休克复苏后的免疫抑制状态可能与CD 4 ^+ CD 25 ^+调节性T细胞有关。