Research on Apoptosis after Hemorrhagic Shock -Hypertonic Saline Resuscitation-

失血性休克后细胞凋亡的研究-高渗盐水复苏-

• 批准号: 12671498
• 负责人: MURAO Yoshinori
• 金额: $ 1.98万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671498/
• 关键词: Hemorrhagic Shock; Hypertonic Saline; Apoptosis; TUNEL; DNA ladder; caspase-3; DNA ladder; Caspase-3

Male Sprague-Dawley rats were hemorrhaged until a mean arterial pressure (MAP) of 40 +/- 5 mmHg was reached. This level of hypotension was sustained for 1 h, and the animals were randomly assigned to the following groups by resuscitation methods. Control, Sham, HS + SB : (4 mg/Kg of 7.5 % NaCl solution: HS) and shed blood (SB) at the same time, 2LR + SB : hemorrhage shock for 1 h and resuscitated with Lactated Ringer's solution (2 times the volume of the shed blood) + SB, 3LR (3 times the volume of the shed blood). Lungs were harvested and total histology scores were examined at 48 h after hemorrhage and resuscitation. HS + SB group significantly prevented lung damage compared with other groups (p < O.05). The effect of hypertonic saline resuscitation on the time course of intestinal damage and apoptosis after hemorrhagic shock in mice was invesigated. After anesthesia, male BALB/c mice were hemorrhaged to the mean arterial pressure of 40 +/- 5 mmHg for 90 min.Resuscitation was made by … More HS + SB or 2LR + SB. Intestinal injury was devised and based on the extent of the vacuolation at basal area of the intestinal villi. Apoptosis score of the small intestines was examined by TUNEL method and DNA ladder assay. Activation of caspase-3 expression was assessed, by Western blot. Hemorrhagic shock and resuscitation induced the apoptosis in 2LR + SB at 2 h and intestinal damage in 2LR + SB at 6h compared to other groups significantly (p < 0.01) Apoptosis index of HS + SB at 2h group was significantly reduced compared to 2LR+SB at 2h (p < 0.01) and also intestinal damage of HS + SB at 6h group was significantly reduced compared to 2LR + SB at 6h (p < 0.01).Activation of caspase-3 of HS + SB at 2h group was reduced compared to 2LR + SB at 2h group. Conclusions: Hypertonic saline resuscitation reduced the apoptosis and intestinal damage after hemorrhagic shock and resuscitation. Thus, the effect of hypertonic saline resuscitation toward apoptosis may have important role in hemorrhagic shock and resuscitation. Less

雄性Sprague-Dawley大鼠失血直至平均动脉压(MAP)达到40+/-5mmHg。这种低血压水平持续1小时，通过复苏方法将动物随机分配到以下组。 Control，Sham，HS+SB：（4 mg/Kg 7.5% NaCl溶液：HS）同时流血（SB），2LR+SB：失血性休克1小时，用乳酸林格氏液（流血量的2倍）+SB、3LR（流血量的3倍）复苏。出血和复苏后48小时收获肺并检查总组织学评分。与其他组相比，HS + SB 组显着预防了肺损伤（p < O.05）。研究高渗盐水复苏对失血性休克小鼠肠道损伤和细胞凋亡时程的影响。麻醉后，雄性 BALB/c 小鼠失血至平均动脉压 40 +/- 5 mmHg，持续 90 分钟。通过 HS + SB 或 2LR + SB 进行复苏。肠损伤是根据肠绒毛基底区域空泡形成的程度设计的。采用TUNEL法和DNAladder法检测小肠细胞凋亡评分。通过蛋白质印迹评估 caspase-3 表达的激活。失血性休克和复苏诱导 2LR+SB 2 h 组细胞凋亡和 2LR+SB 6h 肠道损伤与其他组相比显着降低（p < 0.01） HS+SB 2h 组细胞凋亡指数较 2LR+SB 2h 组显着降低（p < 0.01），HS + SB 6h 组肠道损伤也较其他组显着降低（p < 0.01）。 6h时2LR+SB(p < 0.01)。与2h组2LR+SB相比，2h组HS+SB的caspase-3活化降低。结论：高渗盐水复苏可减少失血性休克复苏后的细胞凋亡和肠道损伤。因此，高渗盐水复苏对细胞凋亡的影响可能在失血性休克和复苏中具有重要作用。较少的

项目成果

Hata M, Murao Y, Konobu T, Miyamoto S, Konishi N: "Hypertonic Saline Without Transfusion Does not Attenuate Lung Damage After Hemorrhagic Shock in Rats"5th World Congress on Trauma, Shock, Inflammation and Sepsis Editor Faist E. Monduzzi Editore Italy. 97

Hata M、Murao Y、Konobu T、Miyamoto S、Konishi N：“不输血的高渗盐水不会减轻大鼠失血性休克后的肺损伤”第五届世界创伤、休克、炎症和脓毒症大会编辑 Faist E. Monduzzi Editore 意大利。

Hata M., Murao Y., Konobu T., Miyamoto S., Konishi N.: "Hypertonic Saline Without Transfusion Does not Attenuate Lung Damage After Hemorrhagic Shock in Rats"5 th World Congress on Trauma, Shock, Inflammation and Sepsis Editore Faist E Monduzzi Editore, It

Hata M.、Murao Y.、Konobu T.、Miyamoto S.、Konishi N.：“不输血的高渗盐水不会减轻大鼠失血性休克后的肺损伤”第五届世界创伤、休克、炎症和败血症大会 Editore Faist

Hata M, Murao Y, Konobu T, Miyamoto S, Konishi N.: "Hypersonic Saline Without Transfusion Does not Attenuate Lung Damage After Hemorrhagic Shock in Rats"5th World Congress on Trauma, Shock, In flammation and Sepsis Editor Faist E. Monduzzi Editore Italy.

Hata M、Murao Y、Konobu T、Miyamoto S、Konishi N.：“不输血的高超音速盐水不会减轻大鼠失血性休克后的肺损伤”第五届世界创伤、休克、炎症和脓毒症大会编辑 Faist E. Monduzzi Editore

Hurao Y, et al: "Hypertonic saline resuscitation reduces apoptosis of the small intestine after hemorragic shock in rats"Intensive Cave Medicine. 26. S268 (2000)

Hurao Y 等人：“高渗盐水复苏可减少大鼠失血性休克后小肠细胞凋亡”《强化洞穴医学》。

村尾佳則 他: "出血性ショック後の高張食塩液投与による肺障害防御効果の検討-肺障害と小腸アポトーシスの関係について-"日本救急医学会雑誌. 11. 485 (2000)

Yoshinori Murao等：“检查高渗盐水对失血性休克后肺损伤的保护作用-关于肺损伤和小肠细胞凋亡之间的关系-”日本急救医学会杂志11. 485 (2000)。