Research on Apoptosis after Hemorrhagic Shock -Hypertonic Saline Resuscitation-

将雄性Sprague-Dawley大鼠饲养至平均动脉压（MAP）达到40 +/-5 mmHg。该低血压水平持续1小时，通过复苏方法将动物随机分配至以下组。对照组、假手术组、HS + SB组：（4 mg/Kg的7.5%NaCl溶液：HS）和同时放血（SB），2LR + SB组：失血性休克1 h，用乳酸林格氏液（2倍放血体积）+ SB复苏，3LR组（3倍放血体积）。在出血和复苏后48 h收获肺并检查总组织学评分。HS + SB组与其他组相比显著防止肺损伤（p <0.05）。本实验观察了高渗盐水复苏对失血性休克小鼠肠道损伤及细胞凋亡的影响。麻醉后，将雄性BALB/c小鼠麻醉至40 +/-5mmHg的平均动脉压90分钟。 关于我们 根据小肠绒毛基底部空泡化程度设计肠损伤模型。TUNEL法和DNA Ladder法检测小肠细胞凋亡评分。通过蛋白质印迹法评估半胱天冬酶-3表达的活化。失血性休克和复苏组2LR + SB在2 h时细胞凋亡明显增加，2LR + SB在6 h时肠组织损伤明显，与其他组比较差异有显著性HS + SB 2 h组细胞凋亡指数明显低于2LR+SB 2 h组（P < 0.01与2LR + SB组相比，HS + SB组在6 h时的肠损伤也显著减少（p < 0.01 HS + SB 2 h组caspase-3活性低于2LR + SB 2 h组（P < 0.01）。结论：高渗盐水复苏可减轻失血性休克复苏后肠组织细胞凋亡，减轻肠组织损伤。因此，高渗盐水复苏对细胞凋亡的影响可能在失血性休克复苏中起重要作用。少

Male Sprague-Dawley rats were hemorrhaged until a mean arterial pressure (MAP) of 40 +/- 5 mmHg was reached. This level of hypotension was sustained for 1 h, and the animals were randomly assigned to the following groups by resuscitation methods. Control, Sham, HS + SB : (4 mg/Kg of 7.5 % NaCl solution: HS) and shed blood (SB) at the same time, 2LR + SB : hemorrhage shock for 1 h and resuscitated with Lactated Ringer's solution (2 times the volume of the shed blood) + SB, 3LR (3 times the volume of the shed blood). Lungs were harvested and total histology scores were examined at 48 h after hemorrhage and resuscitation. HS + SB group significantly prevented lung damage compared with other groups (p < O.05). The effect of hypertonic saline resuscitation on the time course of intestinal damage and apoptosis after hemorrhagic shock in mice was invesigated. After anesthesia, male BALB/c mice were hemorrhaged to the mean arterial pressure of 40 +/- 5 mmHg for 90 min.Resuscitation was made by … More HS + SB or 2LR + SB. Intestinal injury was devised and based on the extent of the vacuolation at basal area of the intestinal villi. Apoptosis score of the small intestines was examined by TUNEL method and DNA ladder assay. Activation of caspase-3 expression was assessed, by Western blot. Hemorrhagic shock and resuscitation induced the apoptosis in 2LR + SB at 2 h and intestinal damage in 2LR + SB at 6h compared to other groups significantly (p < 0.01) Apoptosis index of HS + SB at 2h group was significantly reduced compared to 2LR+SB at 2h (p < 0.01) and also intestinal damage of HS + SB at 6h group was significantly reduced compared to 2LR + SB at 6h (p < 0.01).Activation of caspase-3 of HS + SB at 2h group was reduced compared to 2LR + SB at 2h group. Conclusions: Hypertonic saline resuscitation reduced the apoptosis and intestinal damage after hemorrhagic shock and resuscitation. Thus, the effect of hypertonic saline resuscitation toward apoptosis may have important role in hemorrhagic shock and resuscitation. Less