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Development of a feed-back controlled culture system to maximize calcification of tissue-engineered hone promoted by strain-induced fluid flow

开发反馈控制培养系统，以最大限度地提高应变诱导流体流动促进的组织工程磨石的钙化

基本信息

批准号：
18500343
负责人：
TANAKA Shigeo
金额：
$ 2.57万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500343/
关键词：
Tissue engineering Regenerative medicine Bone Osteoblast Mechanical stimulation Biomechanics Cell Calcification

项目摘要

Stem cells have a potential to differentiate to specific organ cells and could be utilized to regenerate a lost organ with an appropriate scaffold in culture. This technology called tissue engineering or regenerative medicine is a promising strategy with a high biocompatibility avoiding the ethical problems in cadaveric donor transplantation and living donor transplantation Tissue-engineered bone composed of osteoblasts, a scaffold, and simulative hums was studied and developed in the past years, however it is still difficult to obtain sufficient calcification in vitro, comparing with natural bones. With this low mechano-compatibility, in particular a shortage of initial stiffness and toughness, tissue-engineered bone is not applicable to implantation to loaded bone sites. The purpose of this project is to development of a feed-back controlled culture system to maximize the calcification of tissue-engineered bone promoted by mechanical stimulation in fain of strain-induced fluid flow. This culture system is expected to provide better mechano-compatibility to an engineered bone before implantation. In this two-year project (2006-2007), two elemental technologies for the system were developed. In 2006, an optical device for non-destructive monitoring of in vitro calcification was developed using near-infrared light Using this device, the calcification of tissue-engineered bone composed of a type I collagen sponge and rat-primary cultured osteoblasts was monitored for 42 days in culture. In 3307, in the purpose of determination of initial condition of mechanical stimulation, a compact optical device was developed to observe concentration changes in intracellular calcium in osteoblasts populated in an engineered bone just after mechanical stimulation. These achievements should contribute to completion of the feed-hack controlled culture system for tissue-engineered bone.

干细胞具有分化为特定器官细胞的潜力，并且可以在培养中利用适当的支架再生丢失的器官。组织工程或再生医学是一种具有高度生物相容性的有前途的策略，避免了尸体供体移植和活体供体移植的伦理问题。组织工程骨由成骨细胞、支架和模拟hums组成，在过去的几年中被研究和开发，但与天然骨相比，它仍然难以在体外获得足够的钙化。由于这种低的机械相容性，特别是初始刚度和韧性的不足，组织工程骨不适用于植入到负载骨部位。本研究旨在建立一种反馈控制的组织工程骨培养系统，以促进组织工程骨在应力诱导的流体流动中的钙化。这种培养系统有望在植入前为工程骨提供更好的力学相容性。在这个为期两年的项目（2006-2007年）中，开发了该系统的两项基本技术。2006年，开发了一种利用近红外光进行体外钙化非破坏性监测的光学装置。使用该装置，对由I型胶原海绵和大鼠原代培养成骨细胞组成的组织工程骨的钙化进行了42天的培养监测。在3307中，为了确定机械刺激的初始条件，开发了一种紧凑的光学装置，以观察机械刺激后刚植入工程骨中的成骨细胞中细胞内钙的浓度变化。这些研究成果将为进一步完善组织工程骨的反馈控制培养体系奠定基础。