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In vivo measurement of nitric oxide on the surface of rat cremaster muscle arterioles

大鼠提睾肌小动脉表面一氧化氮的体内测量

基本信息

批准号：
18500505
负责人：
MAEDA Junichi
金额：
$ 2.58万
依托单位：
Miyagi University of Education
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500505/
关键词：
nitric oxide concentration in vivo measurements micro electrode rat cremaster muscle 挙睾筋

项目摘要

It has been established that nitric oxide (NO) plays an important role in the regulation of vascular tone. However, NO concentration [NO] in the microcirculation of skeletal muscle has not been determined. In the present study, we measured [NO] in vivo on the surface of the arterioles of the rat cremaster muscle by using NO-specific microelectrodes. The right cremaster muscles of anesthetized male Wistar-Hannover rats were surgically exteriorized using the modified Baez method (Baez S 1973). Images gained through a microscope were divided into a high speed CCD camera and into an image viewing screen with a pair of photodiodes. Vessels were classified into the order of 1A, 2A, 3A and 4A by Wiedeman's ordering system. [NO] was measured directly with a amperometric NO-selective microelectrode (7μm and 30μm, WPI Inc.) through an integrated electrochemical NO detection system (Apollo-4000, WPI). The electrode has a high detection sensitivity of 0.5 - 5.0 pA/nM and a detection limit of 1-3 nM. The microelectrode was placed immediately adjacent to the wall of arterioles on the surface of the cremaster muscle by using a micro-manipulator. [NO] was 916.4 ± 121.6 nM on the surface of 1A arterioles, 468.4 ± 62.5 nM on the 2A, 583.0 ± 131.5 nM on the 3A, and 245.0 ± 138.7 nM on the 4A arterioles. [NO] on the 1A (50 - 100μm diameter) was significantly higher than [NO] on the 4A (10- 30μm). NO concentration on the adjacent surface of the arteriolar wall gradually decreased along with the order of the arteriolar network.

研究表明，一氧化氮（NO）在调节血管张力中起重要作用。然而，骨骼肌微循环中的NO浓度[NO]尚未确定。在本研究中，我们测量[NO]在体内的小动脉的表面上的大鼠提睾肌用NO特异性微电极。使用改良的Baez方法（Baez S 1973），手术取出麻醉雄性Wistar-Hannover大鼠的右提睾肌。通过显微镜获得的图像被分成高速CCD相机和具有一对光电二极管的图像观察屏幕。根据Wiedeman的分类系统将船舶分为1A、2A、3A和4A级。[NO]直接用安培NO选择性微电极（7μm和30μm，WPI Inc.）通过集成的电化学NO检测系统（Apollo-4000，WPI）。该电极具有0.5 - 5.0 pA/nM的高检测灵敏度和1-3 nM的检测限。通过使用显微操作器将微电极直接放置在提睾肌表面上的小动脉壁附近。[NO]在1A小动脉表面上为916.4 ± 121.6 nM，在2A上为468.4 ± 62.5 nM，在3A上为583.0 ± 131.5 nM，在4A小动脉上为245.0 ± 138.7 nM。[NO]1A（50 - 100μm）上的[NO]显著高于4A（10- 30μm）上的[NO]。动脉壁相邻面NO浓度随动脉网排列顺序的变化而沿着逐渐降低。