Functional analyses of heme-DNA coordination complex and its implication for novel functional materials

血红素-DNA配位复合物的功能分析及其对新型功能材料的意义

• 批准号: 18550148
• 负责人: MITA Hajime
• 金额: $ 2.6万
• 依托单位: Fukuoka Institute of Technology (2007)University of Tsukuba (2006)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18550148/
• 关键词: G-quadruplex DNA; function and structure; Redox potential; Axial ligand; heme; dimerization; NMR; thermodynamics; 四重鎮DNA

A G-quadruplex DNA is composed of stacked G-quartets, each of which involves the planar association of four guanine bases. The size and planarity of a G-quartet are well-suited for interacting with a porphyrin ring through π-π stacking. The complexation of G-quadruplex DNAs with porphyrin or metal-porphyrin derivatives has been studied to characterize their molecular recognition of each other as well as to, create catalytic DNAs that exhibit hemoprotein functions. We have demonstrated the formation of a stable coordination complex between heme, the iron(III)-protoporphyrin IX complex, and a parallel G-quadruplex DNA assembled from a single repeat sequence of the human telomere, TTAGGG. The heme-DNA complex was found to be remarkably similar, in various spectroscopic and functional properties, to hemoprotein such as myoglobin.

G-四链体 DNA 由堆叠的 G-四联体组成，每个 G-四联体涉及四个鸟嘌呤碱基的平面关联。 G 四重奏的尺寸和平面度非常适合通过 π-π 堆积与卟啉环相互作用。人们对 G-四链体 DNA 与卟啉或金属卟啉衍生物的络合进行了研究，以表征它们之间的分子识别以及产生具有血红素蛋白功能的催化 DNA。我们已经证明了血红素、铁(III)-原卟啉IX复合物和由人类端粒的单个重复序列TTAGGG组装而成的平行G-四链体DNA之间形成了稳定的配位复合物。人们发现血红素-DNA 复合物在各种光谱和功能特性上与肌红蛋白等血红素蛋白非常相似。

项目成果

Further enhancement of the thermostability of Hydrogenobacter therm ophilus cytochrome c552.

进一步增强嗜热氢杆菌细胞色素 c552 的热稳定性。

• 发表时间: 2006
• 期刊: Biochemistry 45
• 作者: Takahashi;Y.
• 通讯作者: Y.

A spectroscopic index for estimating the age of amber

• DOI: 10.1246/bcsj.79.451
• 发表时间: 2006-03-15
• 期刊: BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
• 影响因子: 4
• 作者: Kimura, H;Tsukada, Y;Yukawa, T
• 通讯作者: Yukawa, T

四重鎖DNAのダイナミクスとポルフィリン誘導体との相互作用

四链体 DNA 动力学及其与卟啉衍生物的相互作用

• 发表时间: 2007
• 期刊: 生物物理 47
• 作者: 織田修司;他6名;堀内昭;山本 泰彦
• 通讯作者: 山本 泰彦

Formation of a complex of 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)-21H,23H-porphyrin with G-quadruplex DNA

• DOI: 10.1021/bi052442z
• 发表时间: 2006-06-06
• 期刊: BIOCHEMISTRY
• 影响因子: 2.9
• 作者: Mita, Hajime;Ohyama, Takako;Yamamoto, Yasuhiko
• 通讯作者: Yamamoto, Yasuhiko

Themlodynamics of dimerization of parallel quadruplex DNAs through the end-to-end stacking of 3'-terminal G-quartets

通过 3 端 G 四联体的端到端堆叠实现平行四链体 DNA 二聚化的热力学

• 发表时间: 2007
• 期刊: Nucleci Acid Symp Ser 51
• 作者: Sakuma;N.
• 通讯作者: N.