Functional analyses of heme-DNA coordination complex and its implication for novel functional materials

点击翻译按钮获取中文摘要

A G-quadruplex DNA is composed of stacked G-quartets, each of which involves the planar association of four guanine bases. The size and planarity of a G-quartet are well-suited for interacting with a porphyrin ring through π-π stacking. The complexation of G-quadruplex DNAs with porphyrin or metal-porphyrin derivatives has been studied to characterize their molecular recognition of each other as well as to, create catalytic DNAs that exhibit hemoprotein functions. We have demonstrated the formation of a stable coordination complex between heme, the iron(III)-protoporphyrin IX complex, and a parallel G-quadruplex DNA assembled from a single repeat sequence of the human telomere, TTAGGG. The heme-DNA complex was found to be remarkably similar, in various spectroscopic and functional properties, to hemoprotein such as myoglobin.