confocal laser-scanning microscope

这是一个建议，共焦激光扫描显微镜在直立配置安装在中央成像单元在研究所解剖学和细胞生物学的医学院（FB 11）的Justus-Liebig-University吉森。它将取代2003年购买的共聚焦激光扫描显微镜，该显微镜不再提供维护，并且不符合申请工作组最先进研究项目的当今要求。它将由解剖学和细胞生物学研究所的中央成像单位运行，该单位还为所有教职员工提供服务。用户的研究项目涵盖细胞生物学的广泛主题，包括气管，胆囊和尿道中刷状细胞和刷状细胞效应器功能的信号转导，男性生殖道中的免疫细胞相互作用，组织特异性分子组成和过氧化物酶体的功能，以及cGMP依赖性信号传导和平滑肌的作用，特别关注男性生殖道。所有这些项目都有一个共同的高度需要的荧光显微成像与歧视的多种荧光染料在高空间分辨率，并允许消除背景荧光，这是使国家的最先进的共聚焦激光扫描显微镜。这些项目通常需要同时对四种甚至七种荧光团进行成像，其中包括在紫外范围内激发的染料，以及在远红范围内发射的染料。这导致需要宽的激发范围，这将由白色光激光器加UV二极管激光器最好地覆盖。快速消除背景自体荧光信号的技术也需要白色激光。活体器官成像在某些项目中起着核心作用，因此需要直立的显微镜配置，额外的浸入式物镜和显微镜载物台上的温控灌注室。

This is a proposal for a confocal laser scanning microscope in an upright configuration to be installed at the central imaging unit at the Institute for Anatomy and Cell Biology of the Medical Faculty (FB11) of the Justus-Liebig-University Giessen. It shall replace a confocal laser scanning microscope bought in 2003 for which maintenance is no longer been offered and which does not meet nowadays requirements of state-of-the-art research projects of the applying working groups. It will be run by the central imaging unit of the Institute for Anatomy and Cell Biology which also offers service to all members of the faculty. The research projects of the users cover a wide spectrum of topics in cell biology, including signal transduction in brush cells and brush cell effector functions in trachea, gallbladder and urethra, immune cell interactions in the male reproductive tract, tissue-specific molecular composition and function of peroxisomes, and role of cGMP-dependent signaling and smooth muscle with particular focus on the male genital tract. All these projects have in common a high need of fluorescence microscopic imaging with discrimination of multiple fluorescent dyes at high spatial resolution and allowing for elimination of background fluorescence, which is enabled by state-of-the-art confocal laser scanning microscopes. The projects require simultaneous imaging of often four and up to seven fluorophores, among them dyes with excitation in the UV range and others with emission in the far-red range. This results in the need of a wide excitation range, which will be covered best by a white light laser plus an UV diode laser. A white light laser is also needed for techniques for fast elimination of background autofluorescence signals. Life imaging of explanted organs plays a central role in some projects, resulting in the need of an upright microscope configuration, additional dip-in objective lenses, and a temperature-controlled perfusion chamber on the microscope stage.