INVESTIGATION OF FISH ROE ALLERGEN AND ITS CROSS-REACTIVITY

在日本，鲑鱼、青鳕和鲱鱼籽等鱼卵是普遍食用的，过去十年报道了许多鱼卵过敏的病例，特别是在儿童时期。然而，本研究旨在鉴定鱼籽过敏原，并探讨不同种类鱼籽间IgE的交叉反应性，结果如下：（1）11种鱼籽中，（鲑鱼：Oncorhynchus凯塔，库页岛泰门：Hucho perryi，虹鳟：Oncorlhynchus mykiss日本红点鲑：Salvelinus leucomaenis，dolly vuuden：Salvelinus malma，银鱼：银纹Theragra chalcogramma、暗鲽Lusky opsetta mochigare、粘比目鱼Microstomus achne、鲭鱼Plecurogrammus azonus、西沙莫胡瓜Spirinchus lanceolatus capelin、毛背野桐Mallotus villosus）含有与抗鲑鱼卵成分兔IgG（抗-β）反应的卵黄蛋白，它们也与鲑鱼卵过敏患者血清中的特异性IgE反应。 关于我们 RA. (2)鱼卵中的主要过敏原是α ′-组分及其相关片段。(3)鲑鱼卵中的IgE成分之间存在交叉反应，鲑鱼卵和青鳕卵之间也存在IgE交叉反应。(4)搅动鲑鱼的氨基酸序列（Mw. 16，000个），并利用虹鳟鱼卵黄原蛋白的全序列通过肽图谱确定了91个残基（约占总序列的60%）的序列。(5)从鲑鱼肝脏中提取mRNA，获得了编码α ′-组分的cDNA，其编码的氨基酸序列与直接肽图分析得到的序列一致。(6)从狭鳕鱼卵中纯化出分子量为17,000的类β ′-组分蛋白，并以大西洋鳕鱼卵黄蛋白原的内部排列为模板，测定了其70%的氨基酸序列，为了解鱼卵过敏和改善过敏患者的生活质量提供了依据。少

Fish roe such as salmon, walleye pollack, and herring roes are popularly consumed in Japan and much cases of fish-roe-allergy, particularly in childhood, have been reported last decade. However; there is little information on fish roe allergens.The objective of this study is to identify fish roe allergens and to investigate the IgE cross-reactivity among various kinds of fish roes.The results were as follows: (1) Eleven kinds of fish roes (Churn salmon: Oncorhynchus keta, Sakhalin taimen: Hucho perryi, rainbow trout: Oncorlhynchus mykiss Japanese char: Salvelinus leucomaenis, dolly vuuden: Salvelinus malma, walleye pollack: Theragra chalcogramma, dusky sole Lepidopsetta mochigare, Slime flounder:Microstomus achne, atka mackerel: Plecurogrammus azonus, sishamo smelt: Spirinchus lanceolatus capelin: Mallotus villosus) contained yolk protein that reacted with anti chuun-salmon-β'-component rabbit IgG (anti-β) and they also reacted with specific IgE in chum salmon roe-allergic patients' se … More ra.(2) The major allergens in salmonid fish roes were β'-component and its related fragments.(3) There were IgE cross-reactivity among salmonid roe β'-components, and the IgE cross--reactivity was also confirmed between churn salmon and walleye pollack roes.(4) Amino acid sequence of churn salmon β'-component (Mw. 16,000) was examined and the sequences of 91 residues (about 60% of the total sequence) were determined by peptide mapping using the complete sequence of rainbow trout viltellogenin.(5) cDNA encoding β'-component was obtained using mRNAs extracted firm chum salmon liver and the encoded amino acid sequence was coincided with the sequence obtained by the direct peptide mapping.(6) β'-component like protein (Mw 17,000) was purified firm walleye pollack roe, and about 70% of the total amino acid sequence was determined using an inside arrangement of Atlantic cod vitellogenin as a template.These findings would contribute understanding fish roe allergy and improvement of QOL of allergic patients. Less