Elucidation of antigenic cross-reactivity mechanism in fish roe allergy

本研究对主要过敏性卵黄蛋白β‘-组分(β’-c)进行了变应原分析，结果如下：(1)用重叠多肽图谱和酶联免疫吸附试验鉴定了β‘-c的5种序列表位。这三种Ig E结合位点位于鱼β‘-cs的高度同源性区域，因此可能是鱼卵的共同表位。(2)从乌鱼鱼子中制备了6种β‘-c亚型，它们与鲑鱼β’-c有交叉反应。(3)成熟鲑鱼肌肉中可观察到1μg/g以上的卵黄蛋白原亚片段，它们具有β‘-c特异性免疫球蛋白E结合能力，提示摄食鱼肉可能诱导对鱼卵过敏。

Allergen analysis of major allergic yolk protein, β'-component (β'-c) was examined in this study and the results were as follows: (1) Five kinds of IgE sequential epitopes in β'-c were identified by overlapped peptide mapping and ELISA. The three kinds of the IgE-binding sites seem to be common epitopes of fish roe, because they were located in high homologous regions of fish β'-cs. (2) Six kinds of β'-c isoforms were prepared from mullet roe and they showed IgE cross-reactivity with salmon β'-c. (3) Vitellogenin subfragment over 1 μg/g were observed in matured salmon muscle and they showed β'-c-specific IgE-binding ability, suggesting food intake of fish meat may induce sensitization to fish roe allergy.