An invention of method to predict reproductive performance of Japanese Black cattle by the evaluation of sperm ability to exhibit flagellar hyperactivation

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1.Induction of sperm flagellar hyperactivation: The treatment with a cell-permeable analog cBiMPS (0.1 mM) for 3 h was effective for the induction of sperm flagellar hyperactivation. This reveals that flagellar hyperactivation is regulated via the cAMP singnaling in the sperm from Japanese Black cattle.2. Characterization of the cAMP signaling: Our previous reports indicate that the cAMP-PKA-SYK signaling cascade is involved in the sperm flagellar hyperactivation in boars. However, this study has suggested that it is regulated via different cAMP signaling cascades in Japanese Black cattle. A possible downstream element of the cAMP signaling has been found as a focal adhesion kinase (FAK)-like protein.3. Biological markers for the prediction of male reproductive performance: The treatment with cBiMPS rapidly induced transformation of motility pattern to curling movement in the sperm from normal bulls. However delayed transformation was observed in the sperm from subfertile bulls which were produced in Gifu Prefecture. On the other hand, relatively larger reduction of the motility was observed during the cBiMPS treatment in the sperm from subfertile bulls which were produced in Hyogo Prefecture. Namely, different responses of spermatozoa to cBiMPS may be valid as biological markers for prediction of male reproductive performance of Japanese Black cattle.4. Biochemical markers for the prediction of male reproductive: performance: Detection levels of 130-kDa, 105-kDa and 94-kDa cAMP-dependently tyrosine-phosphorylated proteins were likely correlated with the percentages of hyperactivated sperm after the treatment with cBiMPS. Thus, these proteins are candidates of biochemical markers for the prediction of male reproductive performance of Japanese Black cattle. Additionally the 94-kDa tyrosine-phosphorylated protein has been identified as a FAK-like protein.