Analysis of diversity and the function of the ileal and jejunal Peyer's patch single follicle

回肠和空肠集合淋巴结单个卵泡的多样性和功能分析

• 批准号: 18580292
• 负责人: YASUDA Masahiro
• 金额: $ 2.43万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580292/
• 关键词: ruminant; primary lymphoid organ; secondary lvmphoid organ; lymphatic follicle; B-cell; immunogrobulin gene; receptor editing; 空腸パイエル板; 回腸パイエル板; レセプターエデイテイング; 多様性

Ruminants' gut-associated lymphoid tissues are broadly classified into ileal and jejunal Peyer's patch. We isolated single lymphatic follicles from ileal and jejunal Peyer's patches and examined thirteen cytokines mRNA expression in the individual follicles using RT-PCR. Especially, IL-2, IL-4 and IL-13 cytokine mRNA was expressed in all examined jejunal Peyer's patch follicle, but not in ileal Peyer's patch follicle. Differences of cytokine mRNA expression facilitate good understanding the differential immune reaction of primary and secondary lymphoid organs. And then, we showed that the immunoglobulin lambda light chain receptor editing could contribute to make the dominant light chain repertoire and very high in-frame ratio of Vlambda-Jlambdal. Finally, we examined morphological characteristics of the full length of calf ileal PP using gross and microscopic anatomical techniques. Most areas of ileal PP consisted of densely packed lymphatic follicles contained very few follicular T-cell and associated with only scant interfollicular areas. However, the proximal end of ileal PP consisted of widely dispersed lymphatic follicles contained many follicular T-cell and associated with large interfollicular areas. The histological architectures of the proximal end of ileal PP strongly resembled those of the secondary lymphoid tissue in calf.

反刍动物的肠道相关淋巴组织大致分为回肠和空肠派伊尔集合淋巴结。我们从回肠和空肠Peyer集合淋巴结中分离单个淋巴滤泡，并使用RT-PCR检测单个淋巴滤泡中13种细胞因子mRNA的表达。IL-2、IL-4和IL-13细胞因子mRNA在空肠Peyer结滤泡中均有表达，而在回肠Peyer结滤泡中无表达。细胞因子mRNA表达的差异有助于更好地理解初级和次级淋巴器官的不同免疫反应。然后，我们发现免疫球蛋白λ轻链受体编辑可能有助于形成优势轻链库和非常高的Vaprida-Japridal框内比率。最后，我们使用大体和显微解剖技术检查了小腿回肠PP全长的形态学特征。回肠PP的大部分区域由密集的淋巴滤泡组成，含有很少的滤泡T细胞，仅与很少的滤泡间区域相关。而回肠PP近端由广泛分布的淋巴滤泡组成，含有大量滤泡T细胞，并伴有较大的滤泡间区。回肠PP近端的组织学结构与小牛次级淋巴组织非常相似。

项目成果

羊の免疫グロブリンλ鎖の解析。Vλ-Jλ1の組み合わせはターミナルである

绵羊免疫球蛋白 λ 链分析。

• 发表时间: 2007
• 作者: 保田 昌宏;Jenne CN;Kennedy LJ;Reynolds JD.
• 通讯作者: Reynolds JD.

Histological studies on the ontogeny of bovine palatine and pharyngee tonsil : germinal center formation, IgG and IgA mRNA expression.

牛腭和咽扁桃体个体发育的组织学研究：生发中心形成、IgG 和 IgA mRNA 表达。

• 发表时间: 2006
• 期刊: Comp Immun Microbiol Infect Dis 29
• 作者: M.;Yasuda;M.;Takanashi;T.;Nasu;T.;Murakami
• 通讯作者: Murakami

羊の免疫グロブリンλ鎖の解析Vλ-Jλの組み合わせはターミナルである

绵羊免疫球蛋白λ链分析 Vλ-Jλ组合末端

• 发表时间: 2007
• 作者: 保田 昌宏、CN Jenne;LJ Kennedy;JD Reynolds
• 通讯作者: JD Reynolds

Dynamics of B-cell repertoire in sheep jejunal and ileal Peyer's patch single folliclee

绵羊空肠和回肠派尔氏集结单卵泡中 B 细胞库的动态

• 发表时间: 2007
• 作者: Yasuda M;Jenne CN;Kennedy LJ;Reynolds JD.
• 通讯作者: Reynolds JD.

Secondary Lymphoid areas in calf ileal Peyer's patch

小腿回肠派尔氏淋巴集结的次级淋巴区域

• 发表时间: 2008
• 期刊: Journal of Veterinary Meclical Science 70
• 作者: M. Takanashi;T. Nasu;T. Murakami;M. Yasuda
• 通讯作者: M. Yasuda