Study for inhibitory regulation of auto reactive T-cell by DC-HIL

DC-HIL对自身反应性T细胞抑制调节的研究

• 批准号: 18580323
• 负责人: BONKOBARA Makoto
• 金额: $ 2.5万
• 依托单位: Nippon Veterinary and Life Science University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580323/
• 关键词: dendritic cells; T-cells; suppression; antigen; co-stimulation

I investigated dendritic cell-mediated antigen specific T-cell proliferation and suppression in this year. The reaction was studied on animals in vivo in previous year ; however, the study was not enough to validate specific reaction of T-cells. Therefore, in vitro analysis system was developed this year (Tamura, et. al. Journal of Veterinary Medical Science 2007). Using antigen prepared from malignant melanoma or malignant lymphoma, dendritic cells were pulsed and proliferation of lymphocytes were examined. Although dendritic cells loaded with antigen prepared from malignant melanoma induced proliferation of lymphocytes, dendritic cells loaded with antigen prepared from malignant lymphoma failed proliferation of lymphocytes. Dendritic cells loaded with antigen prepared from malignant melanoma express undetectable levels of DC-HIL, while that loaded with antigen prepared from malignant lymphoma showed high level expression of DC-HIL. The data suggested that DC-HIL is a molecule closely associates suppression of T cell proliferation. From this study, it was considered that DC-HIL control antigen-specific suppression of T cell and its abnormality may disrupt tolerance of T cells to self-tissues.

今年我研究了树突状细胞介导的抗原特异性T细胞增殖和抑制。去年，该反应在动物身上进行了体内研究；然而，该研究还不足以验证 T 细胞的特异性反应。因此，今年开发了体外分析系统（Tamura等人，Journal of Veterinary Medical Science 2007）。使用从恶性黑色素瘤或恶性淋巴瘤制备的抗原，脉冲树突状细胞并检查淋巴细胞的增殖。尽管负载有从恶性黑色素瘤制备的抗原的树突细胞诱导淋巴细胞增殖，但负载有从恶性淋巴瘤制备的抗原的树突细胞却不能增殖淋巴细胞。负载恶性黑色素瘤制备的抗原的树突状细胞表达不可检测水平的DC-HIL，而负载恶性淋巴瘤制备的抗原的树突状细胞显示高水平的DC-HIL表达。数据表明 DC-HIL 是一种与抑制 T 细胞增殖密切相关的分子。本研究认为DC-HIL控制T细胞的抗原特异性抑制，其异常可能破坏T细胞对自身组织的耐受性。

项目成果

リンパ腫が疑われたイヌ70症例におけるTGRγ鎖およびIgH鎖遺伝子再構成の解析

70只疑似淋巴瘤犬TGRγ链和IgH链基因重排分析

• 发表时间: 2008
• 作者: Tamura;K;Tamura K;Yamada O;Tamura K;Bonkobara M;Yagihara H;Azakami D;Isotani M;Isotani M;植松 洋介
• 通讯作者: 植松 洋介

リンパ腫が疑われたイヌ70症例におけるTCRγ鎖およびIgH鎖遺伝子再構成の解析

70例疑似淋巴瘤犬TCRγ链和IgH链基因重排分析

• 发表时间: 2008
• 作者: Tamura;K;Tamura K;Yamada O;Tamura K;Bonkobara M;Yagihara H;Azakami D;Isotani M;Isotani M;植松 洋介;田村 俊介;植松洋介
• 通讯作者: 植松洋介

Eight cases of malignant melanoma treated with dendritic cell vaccine

树突状细胞疫苗治疗恶性黑色素瘤8例

• 发表时间: 2008
• 作者: Tamura;S
• 通讯作者: S

Generation functional analysis of recombinant self-activated canine caspase 3

重组自激活犬caspase 3的生成功能分析

• 发表时间: 2007
• 作者: Someya;A
• 通讯作者: A

Comparison of dendritic cell-mediated immune responses among canine malignant Cells

犬恶性细胞中树突状细胞介导的免疫反应的比较

• 发表时间: 2007
• 期刊: Journal of Veterinary Medical Science 69
• 作者: Isotani;M;Yagihara H;Uematsu Y;Isotani M;Tamura K
• 通讯作者: Tamura K